Searchable abstracts of presentations at key conferences in endocrinology

ea0086p222 | Metabolism, Obesity and Diabetes | SFEBES2022

Investigating the effects of 5.5 mmoL vs 25 mmoL glucose concentration in culture media on LHCN-M2 cell viability, proliferation, metabolism and differentiation

Brett Ryan , Renshaw Derek , Hodson Leanne , Turner Mark

Introduction: In vitro skeletal muscle cell models are vitally important for investigating the molecular mechanisms of skeletal muscle in metabolic and endocrine diseases, such as obesity and type 2 diabetes. Culture media for skeletal muscle cells can often contain glucose concentrations (GC) five times higher than whatÂ’s considered normal in fasting human plasma, thus is not representative of the in vivo environment. Hyperglycaemia in culture media may...

ea0077p184 | Metabolism, Obesity and Diabetes | SFEBES2021

Does human serum impact differentiation and mitochondrial function of human LHCN-M2 skeletal muscle cells?

Turner Mark , Saini Amarjit , Brown Holly , Anisi Sara , Stewart Claire , Renshaw Derek

Introduction: Skeletal muscle cells enable investigation of myogenesis and metabolic function in vitro. Exposure to human serum can provide insight into the impact of endocrine factors upon differentiation and mitochondrial function of skeletal muscle. The aim of these experiments was to optimise the culture conditions using human serum, which it was hypothesised would enhance myogenesis and mitochondrial function of LHCN-M2 human skeletal muscle cells.<p class="a...

ea0065oc1.3 | Metabolism and Obesity | SFEBES2019

Metabolic and functional effects of fatty acid overload on in vitro tissue engineered skeletal muscle

Turner Mark , Rimington Rowan , Martin Neil , Fleming Jacob , Capel Andrew , Hodson Leanne , Lewis Mark

Introduction: Efficient fuel selection between glucose and fatty acids in insulin-sensitive organs is a key feature which determines metabolic health in humans. In skeletal muscle, lipid storage and utilisation can differ between healthy and diseased individuals, however to date there are limited in vitro models to explore this. Therefore, aim of this work was to investigate the effects of nutrition on engineered skeletal muscle health and metabolism.<p class="abs...