Searchable abstracts of presentations at key conferences in endocrinology

ea0044ep27 | (1) | SFEBES2016

Maternal hypercalcaemia due to CYP24A1 loss of function mutations

Upton Thomas , Florkowski Chris , Phillips Ian , Hunt Penny

Background: Significant changes in calcium metabolism occur during normal pregnancy to meet the needs of the growing fetus. These include a rise in 1,25-dihydroxyvitamin D (1,25-(OH)D2 and consequent suppression of parathyroid hormone (PTH). In spite of this, maternal hypercalcaemia is very uncommon and should prompt further investigation.Clinical case: A 24-year-old primigravida woman was referred for assessment of maternal hypercalcaemia. Sh...

ea0077op2.4 | Adrenal and Cardiovascular | SFEBES2021

Modelling changes in the control, secretion and metabolism of cortisol during and after heart surgery

Galvis Daniel , Zavala Eder , Walker Jamie , Upton Thomas , Lightman Stafford , Angelini Gianni , Gibbison Ben

Control mechanisms of adrenocorticotrophic hormone (ACTH) and cortisol (CORT) secretion and metabolism during acute systemic inflammation (e.g. major surgery) have never been clearly elucidated. We sampled blood every 10 minutes for 12 h during and after coronary artery bypass grafting (CABG) in 10 patients to create profiles of ACTH, CORT and inflammatory mediators and compared these to healthy controls. Patients’ ACTH and CORT responses were classified into one of three...

ea0063p426 | Adrenal and Neuroendocrine Tumours 2 | ECE2019

Validation of 24-hour human adrenal steroid rhythms measured by ULTRADIAN interstitial automated microdialysis: Comparison with plasma

Upton Thomas , Methlie Paal , Russell Georgina , Henne Nina , Tsagarakis Stelios , Kampe Olle , Lightman Stafford , Husebye Eystein

Background: Hormones oscillate in circadian and ultradian rhythms. Consequently, single time point measurements are very difficult to interpret. To address this, we developed an automated system of 24-hour ambulatory microdialysis. This allows measurement of free hormone concentrations in subcutaneous interstitial fluid collected while participants continue normal daily activities. To validate the technique for adrenal steroid hormones, we simultaneously sampled interstitial f...

ea0044p159 | Neuroendocrinology and pituitary | SFEBES2016

Dynamic hormonal diagnostics of acromegaly and Cushing’s disease

Simunkova Katerina , Russel Georgina , Upton Thomas , Husebye Eystein , Methlie Paal , Lovas Kristian , Lightman Stafford

Pituitary and adrenal gland assessment, is one of the most important aspects of the management of a pituitary and adrenal adenoma.Clinical practice varies widely with regard to assessment of pituitary and adrenal status pre- and post-operative. Pre-operative testing includes dynamic testing to assess function which is not practical in the immediate post-operative period. Instead a single morning serum total hormones are measured while a more definitive a...

ea0059oc2.1 | The best of the best | SFEBES2018

24-hour adrenal steroid rhythms are readily detected by ULTRADIAN automated ambulatory microdialysis in man

Upton Thomas , Methlie Paal , Russell Georgina , Tsagarakis Stelios , Kampe Olle , Lightman Stafford , Husebye Eystein

Background: Hormones oscillate in circadian and ultradian rhythms. Single time point samples are difficult to interpret and high frequency measurements are time consuming, expensive and invasive. We developed a minimally invasive technique of ambulatory, automated microdialysis. This allows frequent 24-hour sampling of interstitial fluid while participants continue normal daily activities.Methods: Healthy volunteers (age 18–68, no regular medication...

ea0065p7 | Adrenal and Cardiovascular | SFEBES2019

ULTRADIAN adrenal steroid metabolodynamics successfully discriminates pituitary Cushing’s disease from healthy normal variation

Upton Thomas , Zavala Eder , Fraser Diane , Methlie Paal , Russell Georgina , Tsagarakis Stelios , Kampe Olle , Lightman Stafford , Husebye Eystein

Background: Establishing endocrine diagnoses including pituitary Cushing’s remains challenging with delayed or misdiagnosis being very costly for both patient and the health care system. A key issue is that standard diagnostic tests are typically single time point, single analyte samples, which do not consider dynamic variation intrinsic to endocrine systems. Having previously demonstrated that free tissue hormones correlate strongly with plasma concentrations, we present...

ea0077p139 | Adrenal and Cardiovascular | SFEBES2021

The ULTRADIAN consortium - Ambulatory in vivo micro dialysis in primary adrenal insufficiency, preliminary data

Russell Georgina , Upton Thomas , Zavala Eder , Methlie Paal , Simunkova Katerina , Berinder Katerina , Botusan Ileena , Vassiliadi Dimitria , Tsagarakis Stelios , Kampe Olle , Husebye Eystein , Lightman Stafford , Oksnes Marianne , Bensing Sophie

Patients on long term glucocorticoid replacement therapy have higher rates of morbidity and mortality. One causative factor may be non-physiological cortisol replacement. We have developed as part of the ULTRADIAN consortium ( the U-RHYTHM, an ambulatory bio-sampling device that can collect clinical samples using in-vivo microdialysis every 20 minutes over the 24hour day whilst individuals continue with their normal everyday activities....

ea0077oc4.4 | Adrenal and Cardiovascular | SFEBES2021

24-hour dynamics of free tissue adrenal hormones: A description of healthy normal variation

Upton Thomas , Zavala Eder , Russell Georgina , Oksnes Marianne , Grytaas Marianne , Simunkova Katerina , Botusan Ileana , Berinder Katarina , Bensing Sophie , Vassiliadi Dimitra , Methlie Paal , Kampe Olle , Tsagarakis Stelios , Husebye Eystein , Lightman Stafford

Adrenal hormones possess both circadian and ultradian rhythms, making interpretation of single time point measurements difficult, particularly in the context of suspected endocrine disease. Attempting to capture either normal or pathological rhythms in detail by traditional measurement of blood is impractical and generally unfeasible. However, minimally invasive microdialysis sampling of free tissue hormones coupled with a portable fraction collector (U-RHYTHM), and a targeted...