Searchable abstracts of presentations at key conferences in endocrinology

ea0041gp127 | Neuroendocrinology | ECE2016

Hypothalamic GRK2, via GPR54, modulates puberty onset

Avendano Ma Soledad , Ruiz-Pino Francisco , Vazquez Ma Jesus , Velasco Inmaculada , Heras Violeta , Torres Encarni , Manuel Castellano Juan , Roa Juan , Pinilla Leonor , Tena-Sempere Manuel

The G protein-coupled receptor (GPCR) kinase 2, GRK2, is a ubiquitous serine/threonine protein kinase that is able to phosphorylate and desensitize the active form of several GPCR. Compelling, as yet limited, evidence from in vitro studies have suggested a potential role of GRK2 in mediating desensitization of Gpr54, the canonical receptor for kisspeptins that is abundantly expressed in GnRH neurons. Yet, although kisspeptins have been universally recognized as essent...

ea0041ep789 | Obesity | ECE2016

Intergenerational influence of paternal obesity on metabolic and reproductive health of the offspring: male-preferential impact and potential involvement of Kiss1-mediated pathways

Sanchez-Garrido Miguel Angel , Ruiz-Pino Francisco , Barroso Alexia , Velasco Inmaculada , Heras Violeta , Jesus Vazquez Maria , Castellano Juan Manuel , Roa Juan , Pinilla Leonor , Tena-Sempere Manuel

Obesity and its comorbidities are reaching epidemic proportions. Maternal obesity is known to predispose the offspring to metabolic disorders, independently of genetic inheritance. This intergenerational transmission has also been suggested for paternal obesity during the pre-conception stage, as it appears to have a negative impact on the metabolic and reproductive health of the offspring, likely via epigenetic changes in spermatozoa. However, whether paternal obesity sensiti...

ea0037gp.13.04 | Diabetes and obesity – Translational cardiovascular and obesity | ECE2015

Metabolic and gonadotropic impact of sequential obesogenic insults in the female: influence of the loss of ovarian secretion

Sanchez-Garrido Miguel A , Ruiz-Pino Francisco , Manfredi-Lozano Maria , Leon Silvia , Heras Violeta , Castellano Juan M , Vazquez Maria Jesus , Roa Juan , Romero-Ruiz Antonio , Dieguez Carlos , Pinilla Leonor , Tena-Sempere Manuel

While female fertility is known to be sensitive to conditions of low body fuel reserves, the reproductive consequences of persistent energy excess remain ill defined. Yet, the pandemic proportions of obesity, and its potential impact on the gonadotropic axis, call for a better understanding of this phenomenon. Alike, the influence of ovarian hormones on the pathophysiology of obesity and its complications merits further investigation. In this work, we aimed to elucidate the me...

ea0056oc2.4 | Look who is controlling your gonads! | ECE2018

Novel role of central ceramide signaling in mediating obesity-induced precocious puberty

Heras Violeta , Castellano Juan Manuel , Fernandois Daniela , Velasco Inmaculada , Roa Juan , Vazquez Maria J. , Ruiz-Pino Francisco , Pineda Rafael , Torres Encarnacion , Avendano Maria Soledad , Gaytan Francisco , Pinilla Leonor , Lopez Miguel , Casals Nuria , Tena-Sempere Manuel

Childhood obesity has become a major health problem, which is coupled to different adverse outcomes and diseases. The escalating prevalence of child obesity parallels that of alterations in pubertal timing, also linked to higher disease burden later on life. Yet, the mechanisms underlying for this association remain unfolded. Ceramides, ubiquitous signaling molecules involved in numerous biological processes, have emerged as mediators of metabolic disorders and transmitters fo...

ea0063gp62 | Reproductive Axis | ECE2019

Hypothalamic miR-30 regulates puberty onset via repression of the puberty-suppressing factor, Mkrn3

Castellano Juan M , Heras Violeta , Sangiao-Alvarellos Susana , Manfredi-Lozano Maria , Sanchez-Tapia Maria J , Ruiz-Pino Francisco , Roa Juan , Lara-Chica Maribel , Morrugares-Carmona Rosario , Abreu Ana P , Belsham Denise , Vazquez Maria J , Calzado Marco A , Pinilla Leonor , Gaytan Francisco , Latronico Ana C , Kaiser Ursula B , Tena-Sempere Manuel

Mkrn3, the maternally imprinted gene encoding the makorin RING-finger protein-3, has recently emerged as putative pubertal repressor, as evidenced by central precocity caused by MKRN3 mutations in humans; yet, the molecular underpinnings of this key regulatory action remain largely unexplored. We report herein that the microRNA, miR-30, with three binding sites in a highly conserved region of its 3’-untranslated region (UTR), operates as repressor of Mkrn3 to control pube...