Searchable abstracts of presentations at key conferences in endocrinology

ea0014p345 | (1) | ECE2007

Characterization of facilitative glucose transporters (GLUT) in human thyroid carcinoma cell lines

Ciampi Raffaele , Vivaldi Agnese , Piampiani Pamela , Agate Laura , Salvadori Piero , Del Guerra Alberto , Pinchera Aldo , Elisei Rossella

18FDG-PET is based on the capability of tumor cells to take up glucose. An increment in expression of the glucose transporter 1 (GLUT1) has been observed in thyroid tumors with poor prognosis but very few data are available about the expression of other glucose transporters in thyroid. Here, we study the expression and function of GLUT isoforms 1, 2, 3, 4, 6, 8, 10, 12 in human thyroid carcinoma cell lines ARO and FRO (anaplastic carcinoma), NPA (poorly-differentiated papillar...

ea0014p363 | (1) | ECE2007

Partial redifferentiation of thyroid carcinoma cell lines treated with decitabine and retinoic acid

Vivaldi Agnese , Fabiola Miasaki , Ciampi Raffaele , Agate Laura , Bottici Valeria , Capodanno Alessandra , Collecchi Paola , Pinchera Aldo , Elisei Rossella

In a previous study we demonstrated that retinoic acid (RA) decreased the growth only of thyroid carcinoma cell lines expressing RA receptor β (RAR β) and that decitabine (5-Aza-CdR) re-induced RARβ expression. The aim of this study was to analyze the effects induced by the combined treatment with RA and 5-Aza-CdR in the same thyroid cancer cell lines.We studied the effect of 5-Aza-CdR 800 nM and RA 1 μM on the expression of thyroid s...

ea0014oc1.2 | Thyroid clinical | ECE2007

Expression gene profile may be useful for the diagnosis of thyroid malignancies

Piampiani Pamela , Romei Cristina , Cosci Barbara , Vivaldi Agnese , Ciampi Raffaele , Viola David , Bottici Valeria , Giannini Riccardo , Basolo Fulvio , Pinchera Aldo , Elisei Rossella

Although 20% of follicular neoplasms are papillary thyroid carcinoma (PTC), their cytological diagnosis is not diagnostic. A different profile of gene expression between malignant and benign thyroid tumors has been reported. Aim of this study was to identify a gene expression profile to be used in distinguishing malignant from benign thyroid neoplasms. By real-time RT-PCR we analyzed mRNA expression of 6 thyroid differentiation genes (TTF-1, PAX8, TPO, TSHr, NIS and Tg) and 5 ...