Searchable abstracts of presentations at key conferences in endocrinology

ea0014s4.1 | Gastroenteropancreatic endocrine tumors | ECE2007

Pathological classification of GEP neuroendocrine tumors

Papotti Mauro , Righi Luisella , Volante Marco

In the gastroentero-pancreatic tract, a spectrum of neuroendocrine tumors (NET) exists, including low grade tumors (carcinoid), intermediate grade malignant carcinoid, and high grade poorly differentiated carcinomas of the small and large cell types. In year 2000, the WHO presented a classification scheme for all NETs, but mostly applied to gastroentero-pancreatic tumors. This term carcinoid was replaced by (neuro)endocrine tumor, malignant carcinoid by well differentiated (ne...

ea0032p15 | Adrenal cortex | ECE2013

In vivo and in vitro evidence supporting SSTR/mTOR pathway targeting in adrenocortical cancer

Germano Antonina , Rapa Ida , Duregon Eleonora , Votta Arianna , Ardito Arianna , Volante Marco , Papotti Mauro , Terzolo Massimo

Objective: In vitro data suggest that somatostatin receptors (SSTR) and mTOR pathways might represent reasonable targets for novel therapies, since are involved in growth of adrenocortical carcinoma (ACC). However, in vitro analysis of combination treatments, targeting both mTOR and SSTR as compared to mitotane, and detailed tissue typing mTOR/SSTR pathway are poorly explored in ACC.Methods: This study was designed to characterize the t...

ea0056p116 | Endocrine tumours and neoplasia | ECE2018

Outcome of adjuvant mitotane therapy in patients with adrenocortical carcinoma: the experience of San Luigi Gonzaga Hospital

Puglisi Soraya , Calabrese Anna , Basile Vittoria , Perotti Paola , Pia Anna , Berchialla Paola , Volante Marco , Reimondo Giuseppe , Terzolo Massimo

Background and objectives: The role of adjuvant mitotane therapy after radical surgery for adrenocortical carcinoma (ACC) is still debated. The aim of our study is to assess the effects of adjuvant mitotane on recurrence free survival (RFS) and overall survival (OS) in non-metastatic ACC patients and to evaluate prognostic factors in adjuvant setting.Methods: Retrospective data were collected from 152 patients affected by ACC (stage I-III) referred to ou...

ea0049ep161 | Endocrine tumours and neoplasia | ECE2017

CYP2W1*6 polymorphism as a potential predictive marker of sensitivity to mitotane treatment in adrenocortical carcinoma.

Altieri Barbara , Herterich Sabine , Sbiera Silviu , Volante Marco , Kroiss Matthias , Casa Silvia Della , Pontecorvi Alfredo , Terzolo Massimo , Fassnacht Martin , Ronchi Cristina L.

Mitotane is the only approved drug for the treatment of advanced adrenocortical carcinoma (ACC) and we recently demonstrated that a high expression of cytochrome P450 2W1 (CYP2W1) correlated with response to mitotane. The association between CYP2W1 alleles and a generally increased cancer risk is under debate. Aim of the study was to evaluate the frequency of CYP2W1 polymorphisms and its correlation with the response to mitotane treatment in ACC patients.<p class="abstext"...

ea0070aep206 | Bone and Calcium | ECE2020

Parathyroid carcinoma: An italian multicenter retrospective analysis

Gianotti Laura , Castellano Elena , Lauro Corrado , Piovesan Alessandro , Procopio Massimo , Barale Marco , Palestini Nicola , Quaglino Francesco , Manfrino Luca , Bullara Valentina , Volante Marco , Borretta Giorgio

Parathyroid carcinoma (PC) is a rare neoplasia responsible for about 1% of primary hyperparathyroidism (PHPT). Differently from patients with its benign counterpart, the phenotype of these patients is characterized by severe PHPT and hypercalcemia. The aim of this study was to describe a series of PC casesrecorded in the regional cancer network of Piedmont and Valle d’Aosta, Italy (Rete Oncologica del Piemonte e della Valle d’Aosta) from 2007 to 2017, including 25 ...

ea0026p27 | Adrenal cortex | ECE2011

Do mitotane levels impact on the outcome of patients treated adjuvantly following radical resection of adrenocortical cancer (ACC)?

Ardito A , Al Ghuzian A , Fassnacht M , Daffara F , Leboulleux S , Wortmann S , Zaggia B , De Francia S , Volante Marco , Gonzaga Luigi , Berruti Alfredo , Allolio Bruno , Baudin Eric , Terzolo Massimo

We have demonstrated that adjuvant mitotane prolong recurrence free survival (RFS) in patients with radically resected ACC. Aim of the present study was to correlate mitotane levels with patient outcome in an adjuvant setting in 3 different referral centres in Europe. There were 120 patients (45 W, 75 M, median age 44 years, range 16–76) radically resected for ACC who were treated adjuvantly with mitotane from 1996 to 2010. ACC was stage I in 10 cases, II in 73, III in 31...

ea0081p9 | Adrenal and Cardiovascular Endocrinology | ECE2022

Development and internal validation of a predictive model for the estimation of pheochromocytoma recurrence risk after radical surgery

Lopez Chiara , Parasiliti Caprino Mirko , Bioletto Fabio , Bollati Martina , Maletta Francesca , Caputo Marina , Gasco Valentina , La Grotta Antonio , Limone Paolo , Borretta Giorgio , Volante Marco , Papotti Mauro , Terzolo Massimo , Morino Mario , Pasini Barbara , Veglio Franco , Ghigo Ezio , Arvat Emanuela , Maccario Mauro

Objective: Various features have been identified as predictors of relapse after complete resection of pheochromocytoma, but a comprehensive multivariable model for recurrence risk prediction is lacking. The aim of this study was to develop and internally validate an integrated predictive model for post-surgical recurrence of pheochromocytoma.Methods: The present research retrospectively enrolled 177 patients affected by pheochromocytoma and submitted to ...

ea0059oc1.6 | Translational highlights | SFEBES2018

Germline CYP2W1*6 and CYP2B6*6 polymorphisms as predicting markers of sensitivity to mitotane treatment in advanced adrenocortical carcinoma: a multicentric ENSAT study

Altieri Barbara , Herterich Sabine , Sbiera Silviu , Volante Marco , Francia Silvia De , Casa Silvia Della , Pontecorvi Alfredo , Quinkler Marcus , Kienitz Tina , Mannelli Massimo , Canu Letizia , Chortis Vasileios , Kaltsas Gregory , Kroiss Matthias , Terzolo Massimo , Fassnacht Martin , Ronchi Cristina L

Adrenocortical carcinoma (ACC) is a rare tumor with poor prognosis and the only approved drug for advanced disease is mitotane. The cytochromes P450 (CYP) 2W1 and 2B6 are proposed predicting markers of sensitivity to mitotane treatment. Aim of the study was to evaluate the relationship between CYP2W1 and/or CYP2B6 polymorphisms and response to mitotane in ACC.Methods: We performed a multicentric retrospective study including 182 ACC patients (F/M=121/61)...

ea0070aep5 | Adrenal and Cardiovascular Endocrinology | ECE2020

Cytochrome P450 (CYP) 2W1 affect steroid secretion in adrenocortical cell line and tumor tissues

Altieri Barbara , Sbiera Silviu , Weigand Isabel , Volante Marco , Steinhauer Sonja , Lorey Antonia , Kendl Sabine , Kurlbaum Max , Herterich Sabine , Della Casa Silvia , Colao Annamaria , Terzolo Massimo , Kroiss Matthias , Fassnacht Martin , Ronchi Cristina

Background: The human cytochrome P450 (CYP) 2W1 is involved in the metabolism of several endogenous substrates, including lysophospholipids and fatty acids. Using a polyclonal antibody, we previously demonstrated a high CYP2W1 immunoreactivity in adrenocortical tumors, particularly in those secreting steroids. The aim of the study was to better elucidate the relationship between CYP2W1 and steroid secretion in adrenocortical carcinoma (ACC) H295R cell line and in a larger seri...

ea0014p168 | (1) | ECE2007

Somatostatin receptor immunohistochemistry in neuroendocrine tumors: a proposal of scoring system for clinical characterization and therapy selection

Faggiano Antongiulio , Volante Marco , Pia Brizzi Maria , La Rosa Stefano , Rapa Ida , Ferrero Anna , De Chiara Anna , Righi Luisella , Capella Carlo , De Rosa Gaetano , Dogliotti Luigi , Colao Annamaria , Papotti Mauro

Background: Typing somatostatin receptor (SSTR) expression in neuroendocrine tumors (NETs) is of relevance to target an octreotide-based diagnostic approach and treatment. The expanding use of immunohistochemistry to detect SSTR is to date not paralleled by an accurate methodological setting and standardized interpretation of the results.Objective: A multicentric study was designed to compare SSTR immunohistochemical expression with in vivo scinti...