Searchable abstracts of presentations at key conferences in endocrinology

ea0011p632 | Neuroendocrinology and behaviour | ECE2006

Processing and sorting of pro-opiomelanocortin is an important checkpoint in regulating release of ACTH from secretory vesicles in pituitary cells

Warhurst A , Oliver RL , Davies RA , Pritchard LE , White A

Pro-opiomelanocortin (POMC) is endoproteolysed by prohormone convertase-1 (PC1) to ACTH within the secretory pathway in pituitary cells, where the regulation of ACTH release is essential for mediating the stress response. However POMC is present in the human circulation, indicating that not all POMC is processed. This suggests that regulation of trafficking and processing of POMC are important in determining ACTH release. To investigate this, we analysed steady-state release o...

ea0013oc25 | Neuroendocrinology, reproduction and cardiovascular | SFEBES2007

Manipulation of proprotein convertase 1 expression in AtT20 cells changes proopiomelanocortin processing and trafficking: Implications for ACTH regulatory pathways

Day Tom , Warhurst Anne , Oliver Robert L , Pritchard Lynn E , White Anne

We have previously shown that the ACTH precursor, POMC, is present in human plasma and is the predominant secreted POMC peptide. This suggests that POMC processing is incomplete in neuroendocrine cells and regulation of the post-translational cleavage pathway is important in controlling secretion of ACTH. We have therefore analysed the dynamics of POMC processing in both AtT20 pituitary adenoma cells and primary rat pituitary cells. In both cases, large quantities of POMC, as ...

ea0011p631 | Neuroendocrinology and behaviour | ECE2006

Pro-opiomelanocortin and ACTH, precursors of alphaMSH, are secreted from human melanocytes and keratinocytes and can stimulate melanogenesis

Rousseau K , Kauser S , Prtichard LE , Warhurst A , Oliver RL , Schmitz CA , Thody AJ , Tobin DJ , White A

Pro-opiomelanocortin (POMC) is endoproteolysed to ACTH and alpha MSH which bind to the melanocortin-1 receptor, (MC-1R), and act as principal mediators of human skin pigmentation. In addition there is increasing evidence that alpha MSH has important anti-inflammatory actions in the skin. The aim of this study was to determine the efficiency of processing and the involvement of the different peptides in melanogenesis. To address this question we utilized specific two-site immun...