Searchable abstracts of presentations at key conferences in endocrinology

ea0049oc7.1 | Cardiovascular endocrinology (1) | ECE2017

Estrogens enhance gonadectomy-induced adrenocortical tumor progression in mice

Chrusciel Marcin , Doroszko Milena , Jakobi Meike , Brouze Michal , Ponikwicka-Tyszko Donata , Bernaczyk Piotr , Anisimowicz Slawomir , Wolczynski Slawomir , Toppari Jorma , Huhtaniemi Ilpo , Rahman Nafis

Prepubertally gonadectomized (GDX) wild-type DBA/2J mice develop adrenocortical neoplasms presenting with small spindle-shaped non steroidogenic A-cells and large lipid-laden, steroidogenic and mitotically active B-cells. Neoplastic B-cells overexpress estrogen receptor α (ERα, Ers1) and β (ERβ, Ers2), aromatase (CYP19a1) and produce sex-steroids, resembling gonadal rest tissue. To test the role of estrogen-ER system in adrenocortical tumor progression, GDX...

ea0049gp118 | Endocrine Tumours | ECE2017

Luteinizing hormone receptor mediated GATA4 induction promotes adrenocortical tumorigenesis in gonadectomized mice

Doroszko Milena , Chrusciel Marcin , Stelmaszewska Joanna , Slezak Tomasz , Rivero-Muller Adolfo , Padzik Artur , Anisimowicz Slawomir , Wolczynski Slawomir , Huhtaniemi Ilpo , Toppari Jorma , Rahman Nafis

The role of luteinizing hormone (LH) and its receptor (LHCGR) signaling in the adrenal gland remains unknown. Gonadectomy-induced chronically elevated LH levels trigger neoplastic transformation in genetically susceptible mouse strains (DBA/2J) or transgenic mice expressing the Simian Virus 40 T antigen (SV40Tag) oncogene under inhibin-α promoter (inhα/Tag). In order to study the functional role of LHR and GATA4 in the onset and progression of adrenocortical tumors i...

ea0041oc10.3 | Reproduction & Endocrine Disruption | ECE2016

LHCGR signaling promotes gonadal-like cell differentiation and proliferation in gonadectomy-induced adrenocortical tumorigenesis in mice

Chrusciel Marcin , Doroszko Milena , Jakobi Meike , Brouze Michal , Ponikwicka-Tyszko Donata , Bernaczyk Piotr , Anisimowicz Slawomir , Wolczynski Slawomir , Toppari Jorma , Huhtaniemi Ilpo , Rahman Nafis

Adrenocortical tumorigenesis in prepubertally gonadectomized (GDX) DBA/2J mice has been attributed to chronically elevated luteinizing hormone (LH) and ectopic expression of luteinizing hormone receptor (LHCGR) and GATA-4 transcription factor. We analyzed now the mechanistic role of LH-LHCGR activation in GDX-induced adrenocortical tumorigenesis in intact and ovariectomized (OVX) wild-type (WT) and Lhcgr-/- mice in the DBA/2J genetic background mice, or by ...

ea0014p643 | (1) | ECE2007

The clinical outcomes of stimulation of ovulation in patients with idiopathic hypogonadotropic hypogonadism (IHH) caused by mutations of GnRH receptor

Wolczynski Slawomir , Zbucka Monika , Jarzabek Katarzyna

We report pregnancies obtained after stimulation by gonadotropins in 3 patients with IHH caused by homozygous or compound heterozygous mutations of GnRH receptor: (R139H/R262Q, R139C/ R139C, R139H/ Phe 308 del.);Gonadotropin response to GnRH was observed in the first patient. All the patients were stimulated with gonadotrophins according to the protocole step up with the initial dose of 150IU FSH and 75 IU LH a day. The luteal phase was suported by hCG a...

ea0049ep1104 | Female Reproduction | ECE2017

Serum concentrations of betatrophin and its association with indirect indices of insulin resistance and beta cell function in women with polycystic ovary syndrome

Adamska Agnieszka , Lebkowska Agnieszka , Jacewicz Malgorzata , Krentowska Anna , Hryniewicka Justyna , Wolczynski Slawomir , Gorska Maria , Kowalska Irina

The accumulated data underline the role of betatrophin in glucose homeostasis. Polycystic ovary syndrome (PCOS) is characterized by insulin resistance and high risk of developing prediabetes and diabetes. The aim of our study was to investigate the relationship of serum betatrophin concentrations with indirect indices of insulin resistance and insulin secretion in women with PCOS, comparing to the control group. The study group comprised 43 women with PCOS and 16 controls matc...

ea0041gp183 | Reproduction & Endocrine Disruption | ECE2016

Intima media thickness and brachial artery flow mediated dilatation in women with polycystic ovary syndrome and type 1 diabetes mellitus

Lebkowska Agnieszka , Adamska Agnieszka , Tolwinska Joanna , Jacewicz Malgorzata , Hryniewicka Justyna , Milewski Robert , Wolczynski Slawomir , Bossowski Artur , Gorska Maria , Kowalska Irina

Polycystic ovary syndrome (PCOS) and type 1 diabetes (T1DM) are accompanied by increased risk of atherosclerosis. A higher prevalence of PCOS in T1DM patients has been reported. Brachial artery flow-mediated dilatation (FMD) and intima media thickness of common carotid artery (IMT) are used to detect an early endothelial dysfunction.The aim of our study was to examine IMT and FMD in T1DM women with PCOS. We also estimated the relation of IMT and FMD to c...

ea0037gp.06.04 | Reproduction: Female and PCOS | ECE2015

Phenotypic characteristic of type 1 diabetic women with polycystic ovary syndrome

Lebkowska Agnieszka , Adamska Agnieszka , Karczewska-Kupczewska Monika , Nikolajuk Agnieszka , Otziomek Elzbieta , Milewski Robert , Wolczynski Slawomir , Gorska Maria , Kowalska Irina

Introduction: The prevalence of polycystic ovary syndrome (PCOS) is increased in women with type 1 diabetes (T1DM) in comparison to the general population. It has been suggested that exogenous insulin administered to T1DM women in supraphysiological doses could act as co-gonadotropin and lead to disturbances observed in PCOS.Aim: The aim of this study was to evaluate the phenotypic characteristic and differences between diabetic women with PCOS (T1DM+PCO...

ea0037gp.06.08 | Reproduction: Female and PCOS | ECE2015

Plasma brain derived neurotrophic factor is decreased in women with polycystic ovary syndrome and related to the markers of endothelial dysfunction

Kowalska Irina , Karczewska-Kupczewska Monika , Adamska Agnieszka , Nikolajuk Agnieszka , Lebkowska Agnieszka , Otziomek Elzbieta , Wolczynski Slawomir , Straczkowski Marek

Introduction: Brain derived neurotrophic factor (BDNF) is a neurotrophin which plays a role in neuronal growth and differentiation. Decreased level of BDNF is supposed to play a role in the pathogenesis of the neurodegenerative diseases. Recent data indicate that BDNF could be also involved in angiogenesis. Several lines of evidence support the role of BDNF in glucose metabolism. The decreased level of BDNF are observed in obesity and type 2 diabetes mellitus. Polycystic ovary...

ea0037gp.30.05 | Endocrine tumours and neoplasia – General | ECE2015

Targeted destruction of FSHR-positive cells by lytic peptide Phor21 conjugated with FSHβ subunit in vitro

Chrusciel Marcin , Stelmaszewska Joanna , Ponikwicka-Tyszko Donata , Doroszko Milena , Wolczynski Slawomir , Huhtaniemi Ilpo , Ziecik Adam , Rahman Nafis

FSH receptor (FSHR) expression has been shown in gonadal tumours, as well as in endothelial tumour vessel cells of various cancers. FSHR, due to its transmembrane localization could be a good candidate for receptor-mediated targeted cancer therapy. In recent years, a number of membrane disrupting lytic peptides have been used for receptor-based cancer therapy. In the present study, we characterised the specificity and cytotoxicity of a lytic peptide Phor21 conjugated to three ...

ea0035oc10.5 | Endocrine Tumours | ECE2014

Molecular mechanisms underlying the unexpected promoting effects of mifepristone on murine testicular Leydig cell tumorigenesis

Chrusciel Marcin , Ponikwicka-Tyszko Donata , Stelmaszewska Joanna , Li Xiangdong , Huhtaniemi Ilpo , Toppari Jorma , Wolczynski Slawomir , Rahman Nafis

Progesterone (P4) treatment has been shown to have a clear modulating effect on murine Leydig tumor cell (mLTC-1) function, including downregulation of luteinizing hormone receptor. We hypothesized that P4 would stimulate, whereas an antiprogestine mifepristone (MF) block tumor progression in vivo in a transgenic (TG) murine Leydig cell tumor model (inhibin-α promoter-driven SV40 T-antigen (inhα/Tag)) and act similarly on cell proliferation in vitro...