Searchable abstracts of presentations at key conferences in endocrinology

ea0031p316 | Steroids | SFEBES2013

Validation of CYP11B1 and CYP11B2 regulation by microRNA-24

Diver Louise , Alvarez-Madrazo Samantha , Lin Junjun , Wood Stacy , MacKenzie Scott , Connell John , Davies Eleanor

The CYP11B1 and CYP11B2 genes encode the enzymes responsible, respectively, for the terminal stages of cortisol and aldosterone biosynthesis, and have been implicated in the development of essential hypertension. Previously, we investigated the role of microRNAs in the regulation of these genes and showed in vitro that levels of the adrenally-expressed microRNA-24 (miR-24) inversely correlate with those of CYP11B1 and CYP11B2 mRNA, ...

ea0025oc2.4 | Steroids | SFEBES2011

Altered miR-125 and miR-134 expression in aldosterone-producing adenoma and post-transcriptional regulation of the CYP11B2 gene

Wood Stacy , Ejaz Ayesha , Livie Craig , MacKenzie Scott , Connell John , Davies Eleanor

Essential hypertension is known to have a large genetic component. Variation in the CYP11B2 gene, which encodes the aldosterone synthase enzyme, is associated with excess aldosterone production and hypertension but the causative mechanism remains elusive. miRNAs are a class of post-transcriptional regulatory molecules, implicated in cardiovascular disease, development and tumourogenesis. They act by targeting the 3′ untranslated region (UTR) of mRNAs, inhibiting t...

ea0021p362 | Steroids | SFEBES2009

MicroRNA expression profiling of non-tumorous adrenal tissue and modulation of 11β-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) genes by miR-24

Wood Stacy , MacKenzie Scott , Stewart Paul , Fraser Robert , Connell John , Davies Eleanor

The CYP11B1 and CYP11B2 genes encode 11β-hydroxylase and aldosterone synthase, which catalyse the production of cortisol and aldosterone, respectively, and have been implicated in the development of hypertension. For this study, we wished to investigate the role of microRNAs (miRNAs), a novel class of post-transcriptional gene regulators. To that end, we generated a profile of human adrenal miRNAs to study, and then investigated the action of one adrenal miR...