Searchable abstracts of presentations at key conferences in endocrinology

ea0025oc2.2 | Steroids | SFEBES2011

Anti-inflammatory mechanisms of 5α-reduced glucocorticoids: potential dissociated steroids

Nixon Mark , Yang Chenjing , Rossi Adriano , Walker Brian , Andrew Ruth

We recently showed that 5α-reduced metabolites of corticosterone (B), namely 5α-dihydrocorticosterone (5αDHB) and 5α-tetrahydrocorticosterone (5αTHB), possess similar anti-inflammatory properties to B, but have lesser metabolic effects. Here, we explored the anti-inflammatory mechanisms of these 5α-reduced metabolites in vitro. Data are mean % suppression/induction, compared by one way ANOVA, *P<0.05 versus vehicle.<p class="...

ea0013p169 | Diabetes, metabolism and cardiovascular | SFEBES2007

Are there multiple endogenous glucocorticoids? Chronic Effects of 5α-Tetrahydrocorticosterone in C57BL/6 Mice

Yang Chenjing , Livingstone Dawn , Walker Brian , Kenyon Chris , Andrew Ruth

The rate limiting steps of hepatic glucocorticoid clearance are via A-ring reduction by 5α- and 5β-reductases. Our recent in vitro studies suggested that products of 5α-reduction (eg 5α-tetrahydrocorticosterone) can activate glucocorticoid receptors and hence may contribute to glucocorticoid action in liver. Here we compared the effects of administering 5α-tetrahydrocorticosterone (5αTHB) in vivo with those of corticosterone (B)....

ea0031p317 | Steroids | SFEBES2013

Molecular mechanisms underlying the anti-inflammatory properties of 5α-tetrahydrocorticosterone

Gastaldello Annalisa , Nixon Mark , Yang Chenjing , Saunders Philippa T K , Chapman Karen E , Walker Brian R , Andrew Ruth

Glucocorticoids (GCs) are highly effective anti-inflammatory drugs, however their use is limited by serious side effects. We have previously shown that 5αTHB binds GC receptor (GR) and suppresses inflammation in vitro and in vivo, without affecting metabolism. Here the underlying molecular mechanisms were explored in cell models of ligand-induced GR phosphorylation, nuclear localisation and gene transcription. Data are mean±S.E.M. (th...

ea0021p240 | Neuroendocrinology and behaviour | SFEBES2009

Transgenic disruption of 5alpha-reductase 1 in mice results in a blunted stress response

Livingstone Dawn , Chenjing Yang , Emma Di Rollo , Madina Kara , John Mathews , Lucy Codrington , Christopher Kenyon , Brian Walker , Ruth Andrew

5alpha-Reductase1 (5aR1) catalyses glucocorticoid metabolism, potentially modulating glucocorticoid clearance and therefore affecting the activity of the hypothalamic–pituitary–adrenal (HPA) axis. 5aR1 is up regulated in obesity associated with activation of the hypothalamic–pituitary–adrenal (HPA) axis. Here we investigated if inhibition of 5aR1 impairs glucocorticoid clearance and suppresses the HPA axis.Age-matched male and female ...