Searchable abstracts of presentations at key conferences in endocrinology

ea0086p157 | Adrenal and Cardiovascular | SFEBES2022

Depleting NAD+ pools specifically in the endoplasmic reticulum lumen impairs 11β-hydroxysteroid dehydrogenase activity

Kabli Ali , Heising Silke , Heaselgrave Samuel , Elhassan Yasir , Hardy Rowan , Stromland Oyvind , Ziegler Mathias , Morgan Stuart , Hodson David , Lavery Gareth

Introduction: The endoplasmic reticulum (ER) lumen enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) obtains NADPH from hexose-6-phosphate dehydrogenase to reduce cortisone to the active glucocorticoid cortisol. Cells depleted in NAD+ (parent molecule of NADPH) have impaired 11β -HSD1 activity, which can be rapidly rescued with supplementation of the NAD+ precursor nicotinamide riboside. This suggests the existence of an ER-specific pathway to NAD(P)(H)....