Searchable abstracts of presentations at key conferences in endocrinology

ea0084ps2-09-78 | Thyroid Hormone ACTION | ETA2022

Synthetic analogs of 3-iodothyronamine (T1AM) prevent β-amyloid neurotoxicity in an in vitro model of alzheimer’s disease (AD)

Polini Beatrice , Ricardi Caterina , Carnicelli Vittoria , Tosi Paolo , Bacci Andrea , Rapposelli Simona , Zucchi Riccardo , Chiellini Grazia

We have already reported that synthetic analogs of 3-iodothyronamine (T1AM), a naturally occurring thyroid hormone derivative, share a pleiotropic activity with the endogenous parent compound, including autophagic flux promotion (ATG), neuroprotection, and metabolic reprogramming. Our study aimed to determine whether the T1AM lead analog SG2 and its derivatives SG22 and SG23, developed as prodrugs of SG2, can protect human glioblastoma U87 MG cells from the toxic effects induc...

ea0049gp172 | Obesity | ECE2017

Lipolytic effects of endogenous 3-iodothyronamine (T1AM) and synthetic analog SG-2 in vivo and in cultured adipocytes

Sabatini Martina , Rogowski Michael P , Alpergin Ebru S Selen , Assadi-Porter Fariba , Zucchi Riccardo , Chiellini Grazia

3-Iodothyronamine (T1AM) is a hormone like molecule structurally similar to TH, that has been reported to modulate energy metabolism by favoring lipid over glucose catabolism. To better understand the role played by T1AM on the regulation of lipid metabolism, in the present study we administered spontaneously obese mice with T1AM at two different dosages (10 and 25 mg/kg per day) for 7 days and the effects on body weight (BW) and lipid profiles were examined. In addition a flu...

ea0041ep137 | Calcium and Vitamin D metabolism | ECE2016

Mass Spectrometry coupled with liquid chromatography (HPLC-MS-MS) levels of 25-hydroxy vitamin D (25OHVitD) are associated with prognostic markers of Heart Failure

Saponaro Federica , Saba Alessandro , Frascarelli Sabina , Marcocci Claudio , Cetani Filomena , Passino Claudio , Zucchi Riccardo

Introduction and aims: Heart failure (HF) is a health problem with poor prognosis, despite many treatments available. Vitamin D is the pre-hormone of the active calcitriol (1,25(OH)2D3). It is involved in bone homeostasis, but recent studies suggest extraskeletal functions, including pleiotropic effects on cardiovascular system and a relationship between low levels of 25OHvitamin D and worse HF prognosis. The aims of the study were: 1) to define 25OH-D levels in the HF populat...

ea0070aep857 | Thyroid | ECE2020

Association with 3-iodothyronamine improves the effect of levothyroxine on neurocognitive symptoms in a mouse model of adult-onset hypothyroidism

Rutigliano Grazia , Frascarelli Sabina , Borsò Marco , Bandini Lavinia , Zucchi Riccardo

Adult-onset hypothyroidism is related to anxiety, depression, and impaired neurocognition. Guidelines from all professional societies recommend levothyroxine (LT4) monotherapy as the treatment of choice. However, ~15% hypothyroid patients do not achieve a satisfactory functional level despite TSH within the reference range. The beneficial effects of combining LT4 and liothyronine (LT3) to improve neuropsychiatric functions remains unclear. Recently, 3-iodothyronamine (T1A...

ea0070aep858 | Thyroid | ECE2020

3-iodothyronamine ameliorates ischemia-induced synaptic dysfunction in mouse entorhinal cortex

Tozzi Francesca , Rutigliano Grazia , Borsò Marco , Zucchi Riccardo , Origlia Nicola

Abnormalities in thyroid hormone (TH) availability and/or metabolism have been hypothesized to contribute to Alzheimer’s disease (AD) and to be a risk factor for stroke. Recently, 3-iodothyronamine (T1AM), an endogenous amine putatively derived from TH metabolism, gained interest for its ability to modulate the nervous and the vascular systems. In the present work, we studied the effect of T1AM on ischemia-induced synaptic dysfunction in the entorhinal cortex (EC), a bra...

ea0070ep418 | Thyroid | ECE2020

3-iodothyronamine (T1AM) is taken up and rapidly metabolized in cell culture medium only at low concentration

Saponaro Federica , Borsò Marco , Verlotta Sara , Bandini Lavinia , Saba Alessandro , Ghelardoni Sandra , Zucchi Riccardo

3-iodothyronamine (T1AM) is rapidly catabolized to iodothyroacetic acid after few hours by serum proteins. To avoid a rapid metabolism during any treatment, DMEM devoid of FBS has been used, inducing changes in the cell culture environmental. We evaluated the uptake and the metabolism of different concentrations of T1AM, in presence of supplemented medium, and the presence of 3-iodothyroacetic aldehyde (Ald), a putative intermediate of T1AM catabolism to TA1.<p ...

ea0032p744 | Obesity | ECE2013

Modulation of gene expression by 3-iodothyronamine: evidence of a lipolytic pattern

Mariotti Veronica , Ioffrida Caterina , Melissari Erika , Russo Manuela Di , Frascarelli Sabina , Pellegrini Silvia , Zucchi Riccardo

3-Iodothyronamine (T1AM) is a recently discovered compound which can be regarded as a novel hormone, since it is an endogenous relative of thyroid hormone with systemic distribution, which does not interact with thyroid hormone receptors, but rather with specific G protein-coupled receptors. T1AM has been reported to modulate energy metabolism, and in rodents chronic T1AM treatment has been associated with lipolysis and decreased body weight. T...

ea0084ps2-09-85 | Thyroid Hormone ACTION | ETA2022

3-Iodothyroacetic acid release in a mouse model of ischemia-induced synaptic dysfunction

Ricardi Caterina , Bertolini Andrea , Tozzi Francesca , Polini Beatrice , Rutigliano Grazia , Zucchi Riccardo , Chiellini Grazia , Saba Alessandro , Origlia Nicola

The endogenous thyroid hormone derivative 3-iodothyronamine (T1AM), and its metabolite 3-iodothyroacetic acid (TA1) are known to stimulate learning and induce hyperalgesia in mice. Recently, it has been demonstrated that exogenous T1AM is able to rescue synaptic impairment after transient ischemia in the entorhinal cortex (EC), a brain area crucially involved in learning and memory, and early affected during Alzheimer’s disease. However, i...

ea0063gp180 | Benign Thyroid Disorders | ECE2019

Urinary concentration of iodotyrosines correlates with the severity of iodine deficiency in dehal1 knockout mice

Gonzalez-Guerrero Cristian , Borso Marco , Garcia-Gimenez Jorge , Salas-Lucia Federico , Alikhani Pouya , Saba Alessandro , Zucchi Riccardo , Moreno Jose C

Thyroid hormone (TH) synthesis requires iodine, a scarce element whose recycling is mediated by DEHAL1 through deiodination of iodotyrosines MIT and DIT. In humans, DEHAL1 defects lead to severe congenital hypothyroidism (CH) non-detected by neonatal screening programs, which involves the risk of mental retardation in infants. The timing for establishment of this hypothyroidism remains unknown, but environmental iodine deficiency may represent a triggering factor. Whi...

ea0049gp223 | Thyroid 3 | ECE2017

Endogenous TH metabolite 3-iodothyronamine (T1AM) and synthetic thyronamine-like analogues SG-1 and SG-2 induce autophagy in human glioblastoma cells (U-87MG)

Bellusci Lorenza , Sabatini Martina , Nesi Giulia , Rapposelli Simona , Rossi Leonardo , Lenzi Paola , Fornai Francesco , Zucchi Riccardo , Chiellini Grazia

Endogenous TH metabolite 3-iodothyronamine (T1AM) and recently developed thyronamine-like synthetic analogues SG1 and SG2 have emerged as neuroprotective agents. Autophagy has proved to be an effective therapeutic approach for neurodegenerative diseases. Therefore we investigated whether these compounds can induce autophagy in human glioblastoma cells (U-87MG).Cultured U-87MG cells were initially treated with 1 μM T1AM, SG-1, SG-2 or vehicle for 30&...