Searchable abstracts of presentations at key conferences in endocrinology

ea0005p250 | Steroids | BES2003

Isolated ACTH deficiency precipitated by silent thyroiditis presenting as acute metabolic acidosis

Ajjan R , Andrew J , Spilker C , Ismail A , Nagi D

A 22 years old woman presented with a few days history of nausea, vomiting and general lethargy. She was not on any treatment except the oral contraceptive pill (OCP). She was apyrexial, tachycardic at 140/min with a regular pulse and her blood pressure was 110/70. She was not pigmented. Her plasma sodium was 131 mmol/l, with normal potassium, creatinine and urea. Her arterial blood gas analysis showed a pH of 7.22 and bicarbonate of 12mmol/L. Her TFTs showed a thyrotoxic pict...

ea0003p257 | Steroids | BES2002

Enhanced hepatic A-ring reduction of glucocorticoids in obesity: Regulation by glucocorticoids and insulin

Livingstone D , McInnes K , Walker B , Andrew R

Obesity is associated with hypothalamic-pituitary-adrenal (HPA) axis activation. In obese humans and leptin-resistant obese Zucker rats, we have reported increased excretion of A-ring reduced glucocorticoid metabolites. We have now investigated regulation of hepatic A-ring reductases in the obese Zucker rat.Lean and obese Zucker rats (9wks) were studied either: 3-weeks after adrenalectomy (ADX) or sham surgery; or after 3-weeks treatment with metformin ...

ea0050p277 | Neuroendocrinology and Pituitary | SFEBES2017

Copeptin during hypertonic saline infusion in a polyuria/polydipsia syndrome case series

Boot Christopher , James R Andrew , Tsatlidis Vasileios , Gibb Fraser , Green Fiona , Neely R Dermot G

Introduction: Copeptin is the C-terminal fragment of proAVP and provides an alternative measure of AVP secretion. While direct measurement of AVP during hypertonic saline infusion has been recommended as a diagnostic test for diabetes insipidus (DI), the number of reports examining the utility of copeptin in this context is limited. Here we describe a series of cases where measurement of copeptin during saline infusion has contributed to ...

ea0050p277 | Neuroendocrinology and Pituitary | SFEBES2017

Copeptin during hypertonic saline infusion in a polyuria/polydipsia syndrome case series

Boot Christopher , James R Andrew , Tsatlidis Vasileios , Gibb Fraser , Green Fiona , Neely R Dermot G

Introduction: Copeptin is the C-terminal fragment of proAVP and provides an alternative measure of AVP secretion. While direct measurement of AVP during hypertonic saline infusion has been recommended as a diagnostic test for diabetes insipidus (DI), the number of reports examining the utility of copeptin in this context is limited. Here we describe a series of cases where measurement of copeptin during saline infusion has contributed to ...

ea0065p231 | Metabolism and Obesity | SFEBES2019

Mammary adipose tissue steroid activation and its relevance for breast cancer prognosis

Laforest Sofia , Denver Nina , Homer Natalie ZM , Durocher Francine , Walker Brian R , Andrew Ruth , Tchernof Andre

Background: Adipose tissue dysfunction could partially explain the well-demonstrated association between obesity and survival in breast cancer (BC). Oestrogen and glucocorticoid concentrations and their respective activation enzymes, oestrogenic 17β-hydroxysteroid dehydrogenases (17βHSDs) and aromatase, and 11βHSD1 are increased in adipocyte hyperplasia and hypertrophy, supporting the hypothesis of a potential role for locally generated steroids in obesity-assoc...

ea0038oc4.4 | Diabetes and cardiometabolic complications | SFEBES2015

Hyperinsulinaemia due to inhibition of 5α-reductases is ameliorated by liver-selective glucocorticoid receptor antagonism in diet-induced obesity

Mak Tracy C S , Livingstone Dawn E W , Walker Brian R , Andrew Ruth

Background: 5α-reductase 1 (5αR1) metabolises steroids such as glucocorticoids and androgens, and is highly expressed in murine liver. Genetic disruption of 5αR1 leads to adverse metabolic changes in mice. We hypothesised that dutasteride, a 5αR inhibitor, induces insulin resistance in mice, as in humans, and this effect is underpinned by increased hepatic glucocorticoid action; an experimental paradigm was set up using A-348441, a liver-selective glucocort...

ea0019oc2 | Young Endocrinologist prize session | SFEBES2009

Anti-inflammatory effects of 5 alpha-reduced glucocorticoids in vitro and in vivo

Yang C , Rossi A , Duffin R , Livingstone D , Kenyon C , Walker B , Andrew R

Previously, we have shown that 5α-tetrahydrocorticosterone (5αTHB), the reduced metabolite of corticosterone (B, the main glucocorticoid in rodents), binds to the glucocorticoid receptor, suppresses the HPA axis but has weak in vivo effects on adipose tissue and liver metabolism. Here we have compared the anti-inflammatory effects of B and 5αTHB in three in vitro and in vivo experiments. Cytokine release was measured by cytometric bead array...

ea0019p156 | Diabetes, Metabolism and Cardiovascular | SFEBES2009

Differences in hepatic fatty acid metabolism explain contrasting body weight and steatohepatitis in dietary models of non-alcoholic fatty liver disease in mice

Macfarlane D , Andrew R , Morton N , Nyirenda M , Iredale J , Walker B

Background: In mice, a methionine-choline deficient diet (MCDD) causes steatohepatitis and hepatic insulin resistance. In contrast, a simple choline-deficient diet (CDD) causes liver fat accumulation without steatohepatitis, insulin resistance or weight loss. We hypothesised that differences in liver and adipose fatty acid metabolism underlie the contrasting predisposition to steatohepatitis and hepatic insulin resistance.Methods: C57Bl6 mice (male, aged...

ea0011p345 | Diabetes, metabolism and cardiovascular | ECE2006

Adipose glucocorticoid synthesis: transcription of the 11β-hydroxylase gene in omental but not subcutaneous adipose tissue

MacKenzie SM , Kenyon CJ , Livingstone DEW , Andrew R , Fraser R , Connell JMC , Davies E

The major glucocorticoids (cortisol in man and corticosterone in the rat) play an important role in obesity and its attendant hypertension. However, there is no consistent evidence of glucocorticoid excess in simple obesity, suggesting that its role in the regulation of adipose tissue mass results from a more complex mechanism than adrenal hypersecretion of glucocorticoid. Glucocorticoid biosynthesis is not limited to the adrenal cortex; mRNA and enzymatic activity of 11β...

ea0011p399 | Diabetes, metabolism and cardiovascular | ECE2006

Adrenocortical function in obese Zucker rats

Kenyon CJ , Livingstone DEW , McNeilly AD , Davies E , Andrew R , MacKenzie SM

Adrenal steroids and alterations in steroid metabolism are important factors in the development of obesity in Zucker rats. One might predict, therefore, that the function of the adrenal gland differs between lean and obese Zucker animals. Accordingly, we investigated adrenal gland morphology and function and have considered not only glucocorticoids and HPA activity but also the renin-angiotensin-aldosterone system. Plasma samples and adrenal glands were obtained from young 12 ...