Searchable abstracts of presentations at key conferences in endocrinology

ea0034p350 | Steroids | SFEBES2014

Upregulation of subcutaneous adipose AKR1C3 expression in obese females: evidence for depot- and sex-specific effects

O'Reilly Michael , Gathercole Laura , Arlt Wiebke , Tomlinson Jeremy

Polycystic ovary syndrome (PCOS) is a clinical triad of anovulation, insulin resistance, and androgen excess in women. Adipose androgen generation of testosterone from androstenedione by aldo-ketoreductase type 1C3 (AKR1C3) may contribute to hyperandrogenism, particularly in the setting of obesity. We aimed to determine the effects of BMI and age on AKR1C3 expression in subcutaneous (SC) and omental (OM) fat depots in both women and men.Paired SC and OM ...

ea0023p17 | (1) | BSPED2009

Severe glucorticoid deficiency in 17-hydroxylase deficiency – novel mutation in the CYP17A1 gene

Greening James , Taylor Norman , Arlt Wiebke , Shenoy Savitha

CYP17A1 is a key enzyme of human steroidogenesis, which is unique in that it catalyses two reactions, 17-hydroxylase activity and 17,20 lyase activity. 17-hydroxylase deficiency, a variant of congenital adrenal hyperplasia, results in hypertension and mild glucocorticoid deficiency. Loss of 17,20 lyase activity results in sex steroid deficiency, presenting with undervirilisation in boys (46, XY DSD) and lack of pubertal development in girls. Here we present the cases of two si...

ea0015p154 | Diabetes, metabolism and cardiovascular | SFEBES2008

Dehydroepiandrosterone exerts anti-glucocorticoid action on proliferation, differentiation and insulin sensitivity in human preadipocytes

McNelis Joanne , Gathercole Laura , Stewart Paul , Tomlinson Jeremy , Arlt Wiebke

The adrenal steroid dehydroepiandrosterone (DHEA) and its sulphate ester, DHEAS have been shown to oppose the effects of glucocorticoids, thereby producing beneficial effects on insulin sensitivity in rodent models of diabetes and obesity and in hypoadrenal patients. Glucocorticoids, key regulators of adipose differentiation and insulin sensitivity, are reactivated locally by 11β-hydroxysteroid dehydrogenase type (11β-HSD1) oxoreductase activity, which increases with...

ea0015p308 | Steroids | SFEBES2008

Adrenal emergency is a regular event for treated Addison’s patients

White Katherine , Wass John , Elliott Alyson , Arlt Wiebke

Acute adrenal insufficiency requires immediate treatment with high-dose glucocorticoid and represents a life-threatening emergency. Previous research suggests that primary hypoadrenalism patients (Addison’s disease) are more vulnerable to adrenal emergency than steroid-dependent pituitary patients (Arlt & Allolio 2003).In 2006, we surveyed the membership of the UK Addison’s disease Self-Help Group, comprising 982 hypoadrenalism patients, an...

ea0013p164 | Diabetes, metabolism and cardiovascular | SFEBES2007

DHEA but not DHEAS exerts anti-glucocorticoid action in human preadipocytes

McNelis Joanne , Gathercole Laura , Stewart Paul , Tomlinson Jeremy , Arlt Wiebke

Dehydroepiandrosterone (DHEA) and its sulphate ester DHEAS, have been shown to oppose the effects of glucocorticoids in vivo, thus producing beneficial effects on insulin sensitivity in rodent models of diabetes and obesity as well as in hypoadrenal patients. Glucocorticoids play a key role in regulating fat metabolism and distribution and are reactivated locally by 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) oxoreductase activity, which increases with ...

ea0013p287 | Steroids | SFEBES2007

Unusually mild phenotypic presentation in a family with CYP17A1 deficiency detected by urinary steroid profiling

Arun CS , Ivison HE , Taylor Norman , Arlt Wiebke , Cheetham Tim

The human CYP17A1 enzyme exerts two activities, 17a-hydroxylase and 17,20 lyase, catalysing key steps in human adrenal steroid biosynthesis. An in frame deletion of 3 bp in exon 1 of the CYP17A1 gene, resulting in the loss of phenylalanine in position 53 (F53del), is one of the first CYP17A1 mutations described (JBC 1989, 264:18076). Reported patients have invariably presented with severe hypokalaemic hypertension, reflecting 17α-hydoxylase deficiency, and s...

ea0077lb33 | Late Breaking | SFEBES2021

Complex management of unilateral post-Covid-19 adrenal haemorrhage during pregnancy

Yousuf Quratulain , Elhassan Yasir S , Arlt Wiebke , Jehanzeb Qazi , Krishnasamy Senthil-Kumar , Ronchi Cristina L

Background: Management of large indeterminate adrenal masses detected during pregnancy is challenging due to the risk of malignancy and the obstetric risks of surgical intervention. The spectrum of endocrine-related complications of Covid-19 is expanding. We present a case that highlights the challenging management of a large adrenal mass during pregnancy and draws attention to a rare complication of Covid-19.Case description: 26 yr-old lady presented wi...

ea0081p449 | Reproductive and Developmental Endocrinology | ECE2022

Global impact of PCOS awareness month: challenges and opportunities

Malhotra Kashish , Synn Cuen Pan Carina , Davitadze Meri , Manolopoulos Konstantinos , O'Reilly Michael , Arlt Wiebke , Kempegowda Punith

Background: September is celebrated as PCOS awareness month worldwide and is aimed at improving the lives of those with PCOS, promoting research, and strengthening advocacy efforts. While significant financial and human resources are invested in promotions during this month, to the best of our knowledge, there is no evidence to show the impact of this initiative. In our study, we evaluated the global digital impact of PCOS Awareness Month, tracking commonly used hashtags as su...

ea0081p698 | Reproductive and Developmental Endocrinology | ECE2022

The biochemical investigation of PCOS: a UK wide survey of laboratory practice

M Hawley James , Marrington Rachel , MacKenzie Finlay , Adaway Jo , Taylor Angela , Arlt Wiebke , Keevil Brian

Background: Polycystic ovary syndrome (PCOS) is a heterogenous condition that affects approximately 12% of females. The diagnosis can be complex and biochemistry tests are routinely relied upon to help identify hyperandrogenaemia and to exclude other conditions. However, although national and international guidelines advocate the use biochemistry tests, little information is provided as to which tests should be used and which other endocrinopathies should be excluded.<p cl...

ea0050p283 | Neuroendocrinology and Pituitary | SFEBES2017

Impact of menopause on the natural history of pre-existing prolactinomas

Santharam Sandhya , Tampourlou Metaxia , Arlt Wiebke , Ayuk John , Gittoes Neil , Mtemererwa Brian , Toogood Andrew , Karavitaki Niki

Background: It has been proposed that menopause has a beneficial effect on the natural history of hyperprolactinaemia attributed to prolactinoma. Nonetheless, series systematically assessing outcome in females with prolactinoma who have passed through menopause are very limited.Aim: To investigate the impact of menopause on prolactinomas in patients not on dopamine agonist (DA) treatment.Patie...