Searchable abstracts of presentations at key conferences in endocrinology

ea0007p180 | Reproduction | BES2004

Peroxisome proliferator-activated receptor-gamma (PPARgamma) agonists increase glucose metabolism in a human granulosa cell line (HGL-5)

Berdiaki A , Chatterjee V , Rainey W , Franks S

Ligand-activated peroxisome proliferator-activated receptor gamma (PPARgamma), a nuclear hormone receptor, dimerises with retinoid X receptor(RXR) and acts as a transcription factor translating nutritional, pharmacological and metabolic stimuli into changes in gene expression. PPARgamma is thought to serve several roles in adipocyte differentiation, fatty acid and lipid metabolism, insulin sensitivity and ovarian steroidogenesis. PPARgamma has also been shown to increase gluco...

ea0003p230 | Reproduction | BES2002

FSH alters metabolism and increases chromosomal abnormalities in mouse cumulus-oocyte-complexes matured in vitro

Roberts R , Iatropoulou A , Franks S , Hardy K

In vitro maturation (IVM) of oocytes obtained from medium sized antral follicles could avoid the need for superovulation for in vitro fertilisation. FSH is routinely added to culture media during IVM but the effects on oocyte health are unclear. The aim of this study was to assess the effect of FSH on 1) the metabolic activity of individual mouse cumulus-oocyte-complexes (COCs) matured in vitro,and 2) the genesis of chromosomal abnormalities. 274 mouse COC...

ea0019s71 | Young Endocrinologist prize lecture | SFEBES2009

In search of the genetic basis of polycystic ovary syndrome and its metabolic consequences

Barber T , Wass J , Franks S , McCarthy M

Polycystic ovary syndrome (PCOS) is characterised by reproductive, hyperandrogenic and dysmetabolic features (including insulin resistance). There remain major questions about the basis of the metabolic dysfunction in PCOS and about its genetic aetiology. Having genotyped samples from >460 PCOS cases and >1300 female controls, I recently successfully identified the first genome-sequence variant (the FTO gene) to be implicated in susceptibility to PCOS (Barber <i...

ea0019oc36 | Thyroid, reproduction and endocrine tumours | SFEBES2009

Spatio-temporal expression of bone morphogenetic protein (BMP) antagonists during early follicle development

Fenwick M , Franks S , Stark J , Hardy K

BMPs form part of the TGFβ superfamily, which are important regulators of proliferation and differentiation in many cell types. The intraovarian production of specific BMP members and their receptors in a stage-specific manner is crucial for normal follicle development to proceed. Regulation of BMP signalling pathways can occur by intracellular inhibition of the signal transducers (Smads), or by BMP antagonists that bind extracellular BMPs to prevent or alter ligand–...

ea0019p285 | Reproduction | SFEBES2009

Evidence for discordant granulosa cell proliferation in ovaries of prenatally androgenised sheep: a model for polycystic ovary syndrome

Comim F , Stubbs S , Hardy K , Franks S

Polycystic ovary syndrome (PCOS) is the commonest cause of anovulatory infertility in women. Recent evidence suggests that early folliculogenesis is disordered and may contribute to development of anovulation in PCOS. Early follicle development in PCOS is characterised by increased expression of minichromosome maintenance protein-2 (MCM2), a marker of cell proliferation (Stubbs et al. 2007). Increased recruitment of growing preantral follicles has been reported in the p...

ea0010p73 | Reproduction | SFE2005

Serum concentrations of anti-mullerian hormone (AMH) and inhibin b, in regularly cycling and anovulatory women with polycystic ovaries (PCO)

Al-Qahtani A , Groome N , Themmen A , Goh M , Franks S

Recent studies have shown that serum concentrations of AMH (produced by granulosa cells of the human ovary) are positively related to the number of antral follicles and are negatively correlated with age. Like inhibin B (also a granulosa cell product) AMH is thought to be a useful clinical marker of follicle reserve. Serum AMH concentrations have been reported to be higher than normal in women with polycystic ovary syndrome (PCOS), which is characterised by an excess of antral...

ea0009oc15 | Oral Communication 2: Reproduction and growth | BES2005

Early preantral follicle development in polycystic ovaries (PCO) differs according to ovulatory status

Franks S , Stubbs S , Webber L , Hardy K

Polycystic ovary syndrome (PCOS) is the commonest cause of anovulatory infertility; anovulation in PCOS is characterised by arrest of antral follicle growth that may reflect the abnormal endocrine environment. However, it has recently been shown that preantral follicle development is also abnormal in PCO (Webber et al, Lancet 2003, 362 1017). In that study, the proportion of follicles in the primordial, resting, phase, was reduced, compared to normal ovaries, in tissue from bo...

ea0005oc1 | Reproduction and Development | BES2003

Agonists of peroxisome proliferator-activated receptor-gamma (PPARgamma) regulate steroidogenesis in a human granulosa cell line (HGC-5)

Berdiaki A , Jonard S , Chatterjee V , Rainey W , Franks S

Peroxisome proliferator-activated receptor-gamma (PPARgamma) is one of the isoforms of the PPAR-family of nuclear hormone receptors. Ligand activated PPARs dimerise with retinoid X receptor and act as transcription factors translating nutritional, pharmacological and metabolic stimuli into changes in the expression of genes. PPARgamma is thought to serve several roles in adipocyte differentiation, fatty acid and lipid metabolism and insulin sensitivity. PPARgamma has also been...

ea0004p74 | Reproduction | SFE2002

Localisation of type I IGF receptor and androgen receptor proeins in small preantral follicles of human ovaries

Webber L , Birch R , Flanagan A , Hardy K , Franks S

Little is known about the place of insulin-like growth factors (IGFs) in preantral follicle development, although there is considerable evidence that IGFs are important in antral folliculogenesis. In the rat, isolated large preantral follicles developed better when cultured in vitro in the presence of IGF-I than without (Zhao et al 2001). In the same study, reverse transcriptase polymerase chain reaction identified mRNA for IGF-I and the type I receptor in the granulosa/theca ...

ea0003p229 | Reproduction | BES2002

Expression of mRNA for IGF-1, IGF-2, type 1-IGF receptor, type 2-IGF receptor and insulin receptor in isolated, human preantral follicles

Rice S , Taylor D , Margara R , Franks S , Hardy K

The role of the intra-ovarian insulin-like growth factor (IGF) system in preantral follicle development is unclear. Here we have examined the expression of mRNA for insulin-like growth factor-1 (IGF-1), IGF-2, Type 1-IGF receptor, Type 2-IGF receptor and insulin receptor (IR) in human pre-antral follicles at different stages. Ovarian cortex biopsies (5mm3) were obtained (with informed consent) during gynaecological surgery from six women (age 32.2 plus/minus SD 2.2,...