Searchable abstracts of presentations at key conferences in endocrinology

ea0041gp180 | Reproduction & Endocrine Disruption | ECE2016

An estrogen receptor-dependent pathway is involved in fludioxonil-induced cancer growth and metastasis linked with epithelial mesenchymal transition in cellular and xenografted ovarian cancer models

Kim Cho-Won , Go Ryeo-Eun , Choi Kyung-Chul

Fludioxonil is an antifungal agent used in agricultural applications that is present at measurable amounts in fruits and vegetables. In this study, the effects of fludioxonil on cancer cell viability, epithelial-mesenchymal transition (EMT) and metastasis were examined in BG-1 ovarian cancer cells with estrogen receptors (ERs). BG-1 cells were cultured with 0.1% DMSO (control), 17β-estradiol (E2; 1×10−9 M), or fludioxonil (10−5&#8722...

ea0035p507 | Endocrine disruptors | ECE2014

Transcriptional levels of aryl-hydrocarbon receptor, aryl-hydrocarbon receptor dependent- and cell cycle-related genes were altered by fenhexamid in human ovarian cancer cells expressing estrogen receptors

Go Ryeo-Eun , Yi Bo-Rim , Choi Kyung-Chul

Fenhexamid is one of antifungal agents used in agricultural applications, which are present at measurable amounts in fruits and vegetables. Fenhexamid has been reported to act as an anti-androgen in an androgen receptor reporter assay in engineered human breast cancer cells. Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor, which translocates into nucleus and dimerizes with aryl hydrocarbon receptor nuclear translocator (ARNT). Although function of AR...

ea0056p904 | Endocrine Disruptors | ECE2018

Tumor growth of breast cancer was enhanced by fludioxonil, an antifungal agent, via estrogen receptor-dependent pathways

Kim Cho-Won , Go Ryeo-Eun , Choi Kyung-Chul

Previous studies suggest that environmental factors such as high levels of meat consumption, caffeine, cigarette smoking, pesticides, and endocrine disrupting chemicals (EDCs) may enhance the risk of breast cancer. The fludioxonil is an antifungal agent used in agricultural applications and present at measurable amounts in fruits and vegetables. In this study, the effects of fludioxonil on cancer cell viability and migration ability were examined in MCF-7 and T47D breast cance...

ea0081ep819 | Pituitary and Neuroendocrinology | ECE2022

Demonstration of the effects of asprosin on the sense of smell in female rats with hidden cookie test

Dila Oz Zeynep , Zorlu Go khan , Tan Fatih , Avcu Gaye Go ksu , Kacar Emine , Yasar Abdullah , Serhatlioglu Ihsan , Kelestimur Haluk , Yilmaz Bayram

Purpose: Asprosin is a novel glucogenic adipokine produced by the fibrillin 1 (FBN1) gene that is generated and released by white adipose tissue during fasting. Asprosin hormone has been shown to improve the sense of smell in wild-type mice by activating the OLFR734 receptor and decreasing the time it takes for them to discover hidden food. The purpose of this study was to determine the effects of asprosin on the sense of smell in female rats through the use of a hidden cookie...

ea0063p67 | Calcium and Bone 1 | ECE2019

The effect of steroid hormone on the expression of the calcium-processing proteins in the immature rat brain

Go Seon Myeong , Park Seon Young , Lee Jae-Hwan , Jeung Eui-Bae

The cytosolic calcium concentration is regulated by calcium-processing proteins such as transient receptor potential cation channel subfamily V member 5 (TRPV5), TRPV6, sodium-calcium exchanger 1 (NCX1) and plasma membrane Ca2+ ATPase 1 (PMCA1). Those calcium-processing proteins are important for physiological functions in the brain. The effects of steroid hormones on calcium-processing protein expressions in the brains are undescribed. Thus, the effects of steroid ...

ea0063p994 | Environment, Society and Governance | ECE2019

Effect of octyl-phenol and bisphenol A on calcium signaling in cardiomyocyte differentiation of mouse embryonic stem cells

Lee Jae-Hwan , Go Seon Myeong , Yoo Yeong-Min , Jeung Eui-Bae

Endocrine-disrupting chemicals (EDCs) have similar structures with steroids hormones, which can interfere with hormone synthesis and normal physiological functions of male and female reproductive organs. Sex steroid hormones influence calcium signaling of the cardiac muscle in early embryo development. Progesterone (P4) has been reported to reduce blood pressure. To confirm the effect of P4, octyl-phenol (OP) and bisphenol A (BPA) on early differentiation of mouse embryonic st...

ea0070aep214 | Bone and Calcium | ECE2020

Gene expression of claudins in NCKX3 knockout mouse

Myeong Go Seon , Yi Donglin , Lee Jae-Hwan , Yoo Yeong-Min , Jeung Eui-Bae

Potassium-dependent sodium/calcium exchanger (NCKX3) is expressed in the brain, smooth muscle, the uterus, and intestine. NCKX3 gene plays a role in transporting intracellular calcium and potassium ions across the cell membrane in exchange for extracellular sodium ions. This gene is also associated with calcium metabolism in bones. Tight junctions have functional barriers for material transport and osmotic balance, which are regulated by calcium metabolism. Claudin, o...

ea0073aep554 | Pituitary and Neuroendocrinology | ECE2021

Assessment of a developmental neurotoxicity test using Ki-67 in ReNcell CX cells

Kim Kang Min , Go Seon Myeong , Jeong Sunhwa , Lee Jimin , Jeung Eui Bae

Ki-67 can be solely detected within the cell nucleus, whereas in mitosis, most of the Ki-67 proteins are located on the chromosome surface. Ki-67 is present during all phases of the cell cycle (G1, S, G2, and M), but BrdU is only present in the S phase. This study examined whether it is possible to establish a developmental neurotoxicity test in human neural progenitor cells using Ki-67 instead of BrdU (5-bromo-2’-deoxyuridine). In the present study, Ki-67-expressed ReNce...

ea0073aep588 | Reproductive and Developmental Endocrinology | ECE2021

Establishment of an assay for the effects of neurodevelopmental toxicity using Sox1-GFP cells

Jeong Sunhwa , Park Seon Mi , Go Seon Myeong , Nam Tran Dinh , Lee Jimin , Eui-Bae Jeung

Developmental toxicity tests have been made by embryonic stem cell tests at the European Centre for the Validation of Alternative Methods or by embryonic body test in our laboratory. However, no neuronal-specific developmental toxicity test has been made yet. Therefore, this study was carried out using a 46C cell line, mouse embryonic stem cells with an endogenous Sox1-GFP reporter, to exploit the developmental neurotoxicity test. The expression of Sox1, a marker for neural pr...

ea0070oc8.5 | Environmental Endocrinology | ECE2020

Effects of perinatal exposure to triclosan on neuronal development and behavior of mice

Jeong Sunhwa , Nam Tran Dinh , Myeong Go Seon , Bich Thuy Vo Thi , Jung Eui-Man , Jeung Eui-Bae

There have been raising concerns in the effects of endocrine disrupting chemicals like triclosan (TCS) on the embryo development. Triclosan (TCS) is commonly present in household and personal wash products We hypothesize that exposure to TCS during early stage of development could alter brain development affecting behavior. To test this hypothesis, primary cortical neurons were exposure to TCS with/without estrogen antiestrogen ICI 182780 from day in vitro 1 to 4. We also addr...