Searchable abstracts of presentations at key conferences in endocrinology

ea0065p171 | Metabolism and Obesity | SFEBES2019

5β-reductase (AKR1D1) isoforms differentially regulate natural and synthetic glucocorticoid clearance and glucocorticoid receptor activation in vitro

Appanna Nathan , Arvaniti Anastasia , Gangitano Elena , Morris Karen , George Sherly , Keevil Brian , Tomlinson Jeremy , Nikolaou Nikolaos

Metabolic syndrome and its hepatic manifestation, non-alcoholic fatty liver disease (NAFLD), are increasing in prevalence. Steroid hormones are established regulators of metabolic phenotype. 5β-reductase (AKR1D1) is highly expressed in human liver, inactivating steroid hormones, including glucocorticoids and androgens. The human AKR1D1 gene contains 9 exons; six splice variants have been identified and three lead to functional protein isoforms (AKR1D1-001, -0...

ea0044p216 | Reproduction | SFEBES2016

Androsterone Glucuronide to dehydroepiandrosterone sulphate ratio is higher in obese Caucasian women with Polycystic Ovary Syndrome

Sathyapalan Thozhukat , Ahmed Lina , Keevil Brian , Miller Adrian , Kilpatrick Eric , Atkin Stephen

Objective: Androsterone glucuronide (ADTG) concentrations have been suggested as a more reliable marker of the effects of androgens at the target tissue level and they are significantly elevated in hirsute compared to non-hirsute women with PCOS. This study compared the different precursors of testosterone, including dehydroepiandrosterone sulphate (DHEAS), ADTG and androstenedione in non-obese compared to obese women with PCOS, and in normal subjects and their implications on...

ea0041gp178 | Receptors & Signalling | ECE2016

Salivary testosterone to androstenedione (T/A4) ratio is significantly higher in PCOS patients and accompanied with an adverse metabolic phenotype

Muenzker Julia , Lindheim Lisa , Adaway Jo , Lerchbaum Elisabeth , Pieber Thomas , Keevil Brian , Obermayer-Pietsch Barbara

Background: The aim of this study was to evaluate salivary androgen measurements via liquid chromatography-mass spectrometry as a non-invasive tool for the diagnosis of polycystic ovary syndrome (PCOS) and the assessment of metabolic health.Methods: Saliva and serum samples of 274 patients with PCOS fulfilling Rotterdam criteria and 51 BMI-matched, premenopausal, healthy controls were analysed for steroid hormones using liquid chromatography/mass spectro...

ea0038p24 | Clinical biochemistry | SFEBES2015

Serum cortisol: what is your laboratory measuring?

Hawley James , Owen Laura , Monaghan Phillip , Armston Annie , Chadwick Carrie , Keevil Brian

Background: Accurate measurement of serum cortisol is essential in the investigation of the HPA axis. It has been documented that routine immunoassays are liable to both under- and over-recovery leading to inaccurate results and subsequent inappropriate investigations. This study seeks to provide an up-to-date assessment of the accuracy of the major immunoassay platforms and compares results to a liquid chromatography–tandem mass spectrometry (LC–MS/MS) candidate ref...

ea0038p362 | Reproduction | SFEBES2015

Vitamin D metabolic profiling across pregnancy

Susarla Radhika , Jenkinson Carl , Tamblyn Jennifer , Keevil Brian , Chan Shiao-Yng , Kilby Mark , Hewison Martin

Vitamin D-deficiency during pregnancy has been associated with increased complications of pregnancy including a high risk of pre-eclampsia (PET). Current analysis of vitamin D ‘status’ is based exclusively on analysis of maternal serum 25-hydroxyvitamin D3 (25(OH)D3), the circulating precursor form of vitamin D. We hypothesised that comprehensive profiling of vitamin D metabolites may provide a more accurate determination of vitamin D function i...

ea0038p413 | Steroids | SFEBES2015

Profiling of multiple vitamin D metabolites in a healthy human cohort by high-throughput liquid chromatography–tandem mass spectrometry analysis

Jenkinson Carl , Taylor Angela , Susarla Radhika , Tamblyn Jennifer , Hassan-Smith Zaki , Keevil Brian , Hewison Martin

Although a complex metabolic pathway for vitamin D exists, serum measurement of inactive 25-hydroxyvitamin D3 (25OHD3) continues to be the most common determinant of vitamin D ‘status’. However, several other metabolites contribute to the physiological role of vitamin D, notably the active form 1α,25-dihydroxyvitamin D (1α,25(OH)2D3), inactive 3-epi-25OHD3 and chiral 23R and 24R,25(OH)2D3 metabolites. Quantification of these additional metabolites could pro...

ea0037oc9.3 | Adrenal 2 | ECE2015

Modelling the salivary cortisone to serum cortisol inter-relationship to predict serum cortisol under physiological conditions and after hydrocortisone

Debono Miguel , Harrison Robert , Keevil Brian , Whitaker Martin , Digweed Dena , Arlt Wiebke , Ross Richard

Introduction: Measuring serum cortisol to evaluate the normal circadian rhythm and adequacy of hydrocortisone replacement levels requires multiple readings; an expensive, cumbersome process. Conversely, salivary cortisone, a surrogate marker for free cortisol levels is non-invasive and well suited for out-patient settings. We hypothesised that salivary cortisone predicts circulating cortisol levels and can be used as an alternative marker of serum cortisol in a physiological s...

ea0034p16 | Bone | SFEBES2014

Role of cortisol in the pathogenesis of postmenopausal osteoporosis: relationship to bone structure

Debono Miguel , Bratherton Selina , Paggiosi Margaret , Gossiel Fatima , Keevil Brian , Ross Richard , Eastell Richard

Background: Excess glucocorticoids are well recognised as a cause of osteoporosis; they inhibit osteoblast function and increase osteoblast and osteocyte apoptosis resulting in thinning of the trabeculae. The circadian rhythm of bone turnover, which is linked to cortisol rhythm, is abnormal in osteoporosis. Furthermore, some studies show abnormal cortisol metabolism in osteoporosis. The aim of our study was to evaluate the day–night rhythm of cortisol and to relate cortis...

ea0034p45 | Clinical biochemistry | SFEBES2014

Serum 5HIAA: a better biomarker than urine for detecting and monitoring neuroendocrine tumours?

Adaway Joanne , Dobson Rebecca , Walsh Jennifer , Cuthbertson Daniel , Monaghan Philip , Valle Juan , Devlin Neil , Keevil Brian

Background: 5-hydroxyindole acetic acid (5-HIAA) (a metabolite of serotonin) is used as a marker for patients with serotonin-secreting neuroendocrine tumours. Currently, most laboratories measure 24 h 5-HIAA excretion in urine samples. Urine collections are cumbersome for the patient and impact on their daily activities; they are consequently often poorly performed, leading to over- or under-collection of urine and inaccurate 5-HIAA excretion results. Furthermore, large volume...

ea0034p50 | Clinical biochemistry | SFEBES2014

Reference ranges for salivary steroid measurement during short synacthen tests

Shotton Rohan , Perogamvros Ilias , Keevil Brian , Monaghan Philip , Trainer Peter , Higham Claire

Background: The use of salivary cortisol is well validated in the diagnosis of Cushing’s syndrome but routine use of salivary measurements during dynamic tests of HPA axis sufficiency is uncommon and reference ranges are not established. Salivary cortisol (SalF) and cortisone (SalE) measurements by LC–MS have several advantages including ease of collection and more accurate reflection of unbound serum cortisol levels in conditions of altered CBG levels (e.g. oestroge...