Searchable abstracts of presentations at key conferences in endocrinology

ea0063gp142 | Interdisciplinary Endocrinology 1 | ECE2019

Impact of age and metabolic profile on the definition of 12 circulating steroid reference intervals measured by LC-MS/MS in an Italian healthy adult male cohort

Mezzullo Marco , Di Dalmazi Guido , Fazzini Alessia , Ibarra Gasparini Daniela , Pelusi Carlotta , Pagotto Uberto , Fanelli Flaminia

Background: Steroid profiling by LC-MS/MS is increasingly used in endocrine research, and is being introduced in the clinical practice. Due to the large disagreement with previous immunoassays, and to the enlarged panel of measurable steroids, novel and robust reference intervals (RIs) need to be established. Steroid circulating levels are known to be influenced by several physio-pathological factors, such as age, gender, stress and metabolic status. Therefore, generating unbi...

ea0063gp143 | Interdisciplinary Endocrinology 1 | ECE2019

Impact of age, menstrual phase, menopausal status and metabolic profile on the definition of 12 circulating steroid reference intervals measured by LC-MS/MS in an Italian healthy adult female cohort

Mezzullo Marco , Di Dalmazi Guido , Fazzini Alessia , Ibarra Gasparini Daniela , Gambineri Alessandra , Pagotto Uberto , Fanelli Flaminia

Endocrine clinical practice strongly relies on normative data for the assessment of hormonal imbalance. However, the generation of robust steroids reference intervals (RIs) remains a challenge. As the steroids circulating levels are influenced by many procedural aspects related to blood collection (time, fasting state) and physio-pathological factors, such as age, sex, stress and metabolic status, the need for partitioning RIs is mandatory. Furthermore, unbiased healthy, drug-...

ea0063p502 | Calcium and Bone 2 | ECE2019

Evaluation of bone metabolism in women on aromatase-inhibitors before and after antiresorptive treatment

Vandi Giulia , Zavatta Guido , Repaci Andrea , Di Dalmazi Guido , Altieri Paola , Pagotto Uberto

Introduction: Adjuvant therapy with aromatase-inhibitors (AIs) increases bone loss and fracture risk. Concomitant antiresorptive treatment is currently recommended. The aim of this study was to evaluate the effects of AIs on bone metabolism and mineral density at 12 and 24 months.Methods: Among 400 women on AIs who attended our Endocrinology Unit from 2014 to 2016, we retrospectively evaluated those who initially refused antiresorptive and took cholecalc...

ea0063p515 | Calcium and Bone 2 | ECE2019

Denosumab, alendronate and zoledronate failure to suppress bone resorption markers in a woman affected by severe osteoporosis and subclinical hypercortisolism due to an adrenal adenoma: A case report

Zavatta Guido , Preiato Valentina Lo , Vandi Giulia , Vicennati Valentina , Di Dalmazi Guido , Pagotto Uberto , Altieri Paola

Context: ß-CTX are considered useful markers of response to denosumab and other anti-resorbtive treatments for osteoporosis. We report an unusual case of a completely absent effect of both denosumab and bisphosphonates on bone resorption markers.Case description: In 2017 a 74-year-old woman sought medical attention at the Endocrinology Unit of Policlinico di Sant’Orsola for the evaluation of an adrenal adenoma associated with osteoporosis. Meno...

ea0063p521 | Calcium and Bone 2 | ECE2019

A rare case of cushing disease and hypophosphatasia

Preiato Valentina Lo , Zavatta Guido , Ribichini Danilo , Altieri Paola , Pelusi Carla , Pagotto Uberto

Cushing disease and hypophosphatasia are rare conditions that may be both responsible of an important impairment of bone metabolism with increased fracture risk. We reported for the first time a case of a 43-year-old woman that came at our attention for recent onset of alopecia and amenorrhea. At the anamnesis, she denied any past relevant events and no drug assumption. At the physical examination, she presented rubeosis and facies lunaris and central adipose distribution (wai...

ea0063p1040 | Interdisciplinary Endocrinology 2 | ECE2019

Diabetes and Nivolumab: 2 clinical cases compared

Preiato Valentina Lo , Salvagni Stefania , Ribichini Danilo , Ardizzoni Andrea , Pagotto Uberto , Pelusi Carla

Monoclonal antibodies against the Programmed cell death-1 protein (PD-1) are a new treatment for advanced cancer. Endocrine and metabolic diseases are known to be frequently associated with this class of drugs. We describe two cases of Nivolumab-induced diabetes mellitus.Case 1: A 73-year-old man with a mutated BRAF-V600K advanced stage melanoma was treated with Nivolumab (240 mg IV every 2 weeks). He had normal-weight (BMI: 21.6 Kg/mq), and no previous ...

ea0041ep23 | Adrenal cortex (to include Cushing's) | ECE2016

Ascending aorta dilatation in primary aldosteronism: a new deleterious consequence of aldosterone excess

Vicennati Valentina , Zavatta Guido , Pagotto Uberto , Casadio Elena , Di Dalmazi Guido , Eleonora Rinaldi , Pasquali Renato

Introduction: Primary aldosteronism (PA) features a higher prevalence of cardiovascular events and renal complications as compared with essential hypertension. The effects of aldosterone excess on ascending aorta have never been investigated in human subjects.Methods: From August 2014 to December 2015, we enrolled forty-seven consecutive PA outpatients who had not undergone surgery of the adrenal mass. Hypercortisolism and pheochromocytoma were excluded....

ea0037gp.05.08 | Developmental and paediatric endocrinology | ECE2015

A simple and rapid method for steroid profiling by two dimensional liquid chromatography–tandem mass spectrometry: toward routine application

Fanelli Flaminia , Mezzullo Marco , Fazzini Alessia , Pedercini Matthew , Pasquali Renato , Pagotto Uberto

Steroid testing has a central role in clinical decision-making and in research studies on diseases such as hypercortisolisms, female hyperandrogenism, male hypogonadism or inborn disorders of steroid synthesis. Recently, liquid chromatography–tandem mass spectrometry (LC–MS/MS) proved its superiority to routine immunoassays (IA) in accurately and sensitively measuring low level steroids. However, the replacement of automated IA with LC–MS/MS platforms is limited...

ea0037ep1220 | Clinical Cases–Pituitary/Adrenal | ECE2015

Occurrence of liver failure in post-surgery hypopituitaric patients

Lo Preiato Valentina , Ribichini Danilo , Baccini Margherita , Pasquali Renato , Pagotto Uberto

Multiple pituitary hormone deficit and hypothalamic obesity are common complications after brain surgery for childhood tumors. Despite adequate replacement hormone therapy, obesity develops equally, but liver failure is not usually described. In this study, four subjects who had undergone surgery for brain tumors involving the peri-hypothalamic area when they were nursing or as youths (9 months, 6 years, 9 years and 20 years). They were referred to our Unit when they were 19, ...

ea0035p626 | Female reproduction | ECE2014

Simultaneous determination of salivary androgens by liquid chromatography–tandem mass spectrometry: analytical and biological validation

Mezzullo Marco , Fanelli Flaminia , Benetta Elisa Dalla , Garelli Silvia , Gambineri Alessandra , Pagotto Uberto , Pasquali Renato

The hormone assessment in saliva is emerging as a new valuable tool for clinical and research purposes. Salivary levels are supposed to reflect the free fraction of circulating steroids, thus representing a more informative indicator of their activity. Moreover, saliva collection is not stressful and does not require medical assistance, allowing reliable and multiple sampling in a day. Liquid chromatography–tandem mass spectrometry (LC–MS/MS) technology offers high s...