Searchable abstracts of presentations at key conferences in endocrinology

ea0037gp.05.08 | Developmental and paediatric endocrinology | ECE2015

A simple and rapid method for steroid profiling by two dimensional liquid chromatography–tandem mass spectrometry: toward routine application

Fanelli Flaminia , Mezzullo Marco , Fazzini Alessia , Pedercini Matthew , Pasquali Renato , Pagotto Uberto

Steroid testing has a central role in clinical decision-making and in research studies on diseases such as hypercortisolisms, female hyperandrogenism, male hypogonadism or inborn disorders of steroid synthesis. Recently, liquid chromatography–tandem mass spectrometry (LC–MS/MS) proved its superiority to routine immunoassays (IA) in accurately and sensitively measuring low level steroids. However, the replacement of automated IA with LC–MS/MS platforms is limited...

ea0037ep1220 | Clinical Cases–Pituitary/Adrenal | ECE2015

Occurrence of liver failure in post-surgery hypopituitaric patients

Lo Preiato Valentina , Ribichini Danilo , Baccini Margherita , Pasquali Renato , Pagotto Uberto

Multiple pituitary hormone deficit and hypothalamic obesity are common complications after brain surgery for childhood tumors. Despite adequate replacement hormone therapy, obesity develops equally, but liver failure is not usually described. In this study, four subjects who had undergone surgery for brain tumors involving the peri-hypothalamic area when they were nursing or as youths (9 months, 6 years, 9 years and 20 years). They were referred to our Unit when they were 19, ...

ea0035p626 | Female reproduction | ECE2014

Simultaneous determination of salivary androgens by liquid chromatography–tandem mass spectrometry: analytical and biological validation

Mezzullo Marco , Fanelli Flaminia , Benetta Elisa Dalla , Garelli Silvia , Gambineri Alessandra , Pagotto Uberto , Pasquali Renato

The hormone assessment in saliva is emerging as a new valuable tool for clinical and research purposes. Salivary levels are supposed to reflect the free fraction of circulating steroids, thus representing a more informative indicator of their activity. Moreover, saliva collection is not stressful and does not require medical assistance, allowing reliable and multiple sampling in a day. Liquid chromatography–tandem mass spectrometry (LC–MS/MS) technology offers high s...

ea0035p757 | Obesity | ECE2014

Circulating endocannabinoids are associated with cardiometabolic impairment independently of adiposity in normal weight subjects

Fanelli Flaminia , Belluomo Ilaria , Cuomo Gaia , Mezzullo Marco , Baccini Margherita , Casadio Elena , Pasquali Renato , Pagotto Uberto

Obesity is characterized by hyperactivated endocannabinoid System (ECS) in brain and periphery and by increased levels of circulating endocannabinoids (cECs), anandamide (AEA) and 2-arachidonoylglycerol (2AG). It is still debated whether in absence of obesity cECs are only modulated by adiposity or if they are associated with cardiometabolic parameters.We recruited 155 healthy normal weight (NW, BMI: 18.5–24.9 kg/m2) drug-free volunteers ...

ea0032p16 | Adrenal cortex | ECE2013

Mortality in long-term follow-up patients with progressively increased patterns of subclinical cortisol hypersecretion

Di Dalmazi Guido , Vicennati Valentina , Paccapelo Alexandro , Pagotto Uberto , Pasquali Renato

Background: Subclinical Cushing’s syndrome (SCS) is defined as alterations in hypothalamic–pituitary–adrenal axis without classic signs or symptoms of glucocorticoid excess. This condition has been associated to increased risk of adverse metabolic and cardiovascular outcomes, independently of other potential risk factors. However, is still not known if this condition could lead to a higher mortality respect to non-secreting adrenal masses (NSA).<p class="abs...

ea0022p40 | Adrenal | ECE2010

Higher cortisol response to glucose load in patient with adrenal adenomas and subclinical Cushing (SC)

Vicennati Valentina , di Dalmazi Guido , Casadio Elena , Repaci Andrea , Pagotto Uberto , Pasquali Renato

Aims: SC can be discovered in 5–20% of the incidentalomas. Subclinical secretion is defined by the failure to suppress plasma cortisol after 1 mg-overnight dexamethasone <50 nmol/l, but there is no agreement in literature. The aim of our study was to evaluate cortisol response to oral glucose tolerance test (OGTT) as potential marker of autonomous activity of these adenomas.Methods: Ninety subjects, 47 females and 43 males, underwent the protoco...

ea0022p426 | Endocrine tumours &amp; neoplasia (<emphasis role="italic">Generously supported by Novartis</emphasis>) | ECE2010

Adrenocortical carcinoma (ACC): our experience

Di Dalmazi Guido , Ripani Raffaella , Casadio Elena , Vicennati Valentina , Pagotto Uberto , Pasquali Renato

Aim: ACC is an extremely rare heterogeneous aggressive disease. Clinical presentation are various. The prognosis is very poor. There are limited data to define prognostic markers for survival beyond stage.The aim of this study is to describe the experience of our institution with patients affected by ACC.Material and methods: This study was performed on 13 patients aged between 26 and 76 years, from 1992 to 2009 who turned to our I...

ea0016p503 | Obesity | ECE2008

Expression of the different components of the endocannabinoid system (ECs) in morbid obesity

Vicennati Valentina , Pagotto Uberto , Cervino Cristina , Amenta Enrico , Cariani Stefano , Pasquali Renato

Visceral fat (VAT) may represent the more important site of endocannabinoid dysregulation in obesity. No data are available on the expression of the ECs in morbid obesity. Eleven morbidly obese women, who underwent bariatric surgery, were enrolled and paired samples of VAT and subcutaneous fat tissue (SAT) were obtained from each patient. Real-Time PCR for the gene expression levels of cannabinoid type 1 (CB1) receptor, N-acyl-phosphatidil-ethanolamine (NAPE), and fatty...

ea0016p514 | Obesity | ECE2008

Relationship among stress, weight gain and the activity of the hypothalamic-pituitary-adrenal (HPA) axis

Marconi Valentina , Vicennati Valentina , Corvaglia Stefania , Zampighi Adria , Agostino Romina Di , Pasquali Renato

The aim of this study was: 1) to determine whether there is a correlation between a stressful event, such as the mourning, the hyperactivity of HPA axis, and the fast weight gain 2) to assess whether there is a specific parameter that can predict the susceptibility of individuals to develop metabolic syndrome following a stressful event. We investigated 80 obese women, aged between 23 and 67 years; each subject underwent a clinical evaluation, an OGTT and biochemical, metaboli...

ea0016p620 | Reproduction | ECE2008

Basal INSL3 levels predict LH and androgen response to GnRH analogue in PCOS women

Patton Laura , Gambineri Alessandra , De Iasio Rosaria , Fanelli Flaminia , Pasquali Renato

In a previous study, we demonstrated that the concept of functional ovarian hyperandrogenism (FOH), as documented by the exaggerated 17OHP response to buserelin (GnRH-analogue) does not represent a specific feature of all women with PCOS, being present in less than half of PCOS and is a condition characterized by more severe hyperandrogenemia, glucose-stimulated B-cell insulin secretion and worse insulin resistance. INSL3 is produced in the Leydig cells and at reduced levels i...