Searchable abstracts of presentations at key conferences in endocrinology

ea0050p320 | Obesity and Metabolism | SFEBES2017

Androgen excess is highly prevalent in women with idiopathic intracranial hypertension and is biochemically distinct from polycystic ovary syndromes

O'Reilly Michael , Hornby Catherine , Westgate Connar , Botfield Hannah , Markey Keira , Mitchell James , Jenkinson Carl , Gilligan Lorna , Sherlock Mark , Gibney James , Tomlinson Jeremy , Arlt Wiebke , Sinclair Alexandra

Idiopathic intracranial hypertension (IIH) is a condition of unknown aetiology characterised by raised intracranial pressure, chronic headaches and blindness. Akin to polycystic ovary syndrome (PCOS), IIH patients are almost exclusively obese females of reproductive age. A distinct androgen excess profile has been noted in PCOS. Here, we aimed to delineate androgen metabolism in IIH compared to PCOS and simple obesity.Women w...

ea0063p664 | Interdisciplinary Endocrinology 1 | ECE2019

Differential regulation of 5β-reductase (AKR1D1) expression and activity by glucocorticoids in human and rodent liver

Nikolaou Nikolaos , Morgan Stuart , Larner Dean , Sharp Anna , Raouf Zachariah , Hughes Beverly , Digweed Dena , Whitaker Martin , Ross Richard , Lavery Gareth , Arlt Wiebke , Gathercole Laura , Tomlinson Jeremy

The prevalence of metabolic syndrome and its hepatic manifestation, non-alcoholic fatty liver disease (NAFLD), continues to escalate. Glucocorticoids (GCs) and bile acids (BAs) are established regulators of metabolic phenotype. 5β-reductase (AKR1D1) is highly expressed in human and rodent liver, where it inactivates steroid hormones and catalyses a fundamental step in BA synthesis. We have previously demonstrated that AKR1D1 modulates hepatic GC availability and GC recept...

ea0063p1046 | Pituitary and Neuroendocrinology 3 | ECE2019

Recovery of the hypothalamic-pituitary-adrenal and gonadal axes following trans-sphenoidal adenomectomy, a single centre experience

Pofi Riccardo , Gunatilake Sonali , Macgregor Victoria , Cudlip Simon , Joseph Robin , Lenzi Andrea , Tomlinson Jeremy W , Jafar-Mohammadi Bahram , Isidori Andrea M , Pal Aparna

Hypopituitarism is a potential complication of trans-sphenoidal adenomectomy (TSA). Prediction of pituitary function (PF) recovery would inform hormonal replacement strategies. However, the frequency of re-testing of PF is variable across centres. The aim of this study was to determine rates, time and predictors of 6-weeks (6 w) recovery of hypothalamo-pituitary adrenal (HPA) and gonadal function after TSA.Methods: We performed a single-centre, retrospec...

ea0049oc3.3 | Receptors & Signalling | ECE2017

AKR1C3-mediated adipose androgen generation drives lipotoxicity in polycystic ovary syndrome

O'Reilly Michael , Kempegowda Punith , Walsh Mark , Taylor Angela , Manolopoulos Konstantinos , Allwood William , Semple Robert , Hebenstreit Daniel , Dunn Warwick , Tomlinson Jeremy , Arlt Wiebke

Polycystic ovary syndrome (PCOS) is prevalent metabolic disorder in women, associated with androgen excess and insulin resistance. These two major features are closely correlated, but the direction of causality remains unclear. Aldoketoreductase type 1C3 (AKR1C3) converts the androgen precursor androstenendione to testosterone (T), and is highly expressed in subcutaneous (SC) adipose tissue. We hypothesised that adipose tissue represents an important site linking androgen acti...

ea0049ep682 | Obesity | ECE2017

Suboptimal prediction of advanced fibrotic liver disease by standard non-invasive scoring systems in obese patients undergoing bariatric surgery

Dempster Niall , Watson Michael , Franklin Rachel , Juszczak Maciej , Rickers Lisa , Fletcher Caroline , Sgromo Bruno , Gillies Richard , Cobbold Jeremy , Hodson Leanne , Tomlinson Jeremy , Ryan John

Introduction: Non-Alcoholic Fatty Liver Disease (NAFLD), is present in over 90% of obese patients undergoing bariatric surgery. It is a spectrum of pathology ranging from steatosis through to inflammation (Non-Alcoholic Steatohepatitis, NASH) and fibrosis. Bariatric surgery is currently the most effective treatment for NAFLD, but post-operative hepatic decompensation can occur and therefore pre-operative stratification of liver disease severity is beneficial. Several non-invas...

ea0049ep798 | Neuroendocrinology | ECE2017

Dissecting the androgen excess phenotype of women with idiopathic intracranial hypertension

O'Reilly Michael , Hornby Catherine , Westgate Connar , Botfield Hannah , Markey Keira , Jenkinson Carl , Gilligan Lorna , Sherlock Mark , Gibney James , Tomlinson Jeremy , Arlt Wiebke , Sinclair Alexandra

Abstract: Idiopathic intracranial hypertension (IIH) is a devastating neurological condition, with elevated intracranial pressure of unknown aetiology. IIH is largely a disease of obese females of reproductive age. The clinical phenotype of IIH overlaps with polycystic ovary syndrome (PCOS), with prevalent obesity, hyperandrogenism and anovulation. In this study, we aimed to delineate the androgen excess phenotype of IIH women compared to those with PCOS and simple obesity. Wo...

ea0044oc1.4 | Early Career Oral Communications | SFEBES2016

The urinary steroid metabolome as a non-invasive tool to stage non-alcoholic fatty liver disease

Moolla Ahmad , Amin Amin , Hughes Bev , Arlt Wiebke , Hassan-Smith Zaki , Armstrong Matt , Newsome Philip , Shah Tahir , Van Gaal Luc , Verrijken An , Francque Sven , Biehl Michael , Tomlinson Jeremy

Introduction: Dysregulation of glucocorticoid (GC) metabolism is implicated in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). The only available treatment for NAFLD is weight loss and the gold standard diagnostic test is liver biopsy, which is invasive and resource intensive. NAFLD ranges from simple steatosis, to inflammation (steatohepatitis/NASH), fibrosis and cirrhosis. It may be regarded as the hepatic manifestation of the metabolic syndrome and is strongl...

ea0041gp173 | Receptors & Signalling | ECE2016

The changing ‘steroid metabolome’ across the spectrum of non-alcoholic fatty liver disease

Moolla Ahmad , Amin Amin , Hughes Bev , Arlt Wiebke , Hassan-Smith Zaki , Armstrong Matt , Newsome Philip , Shah Tahir , Van Gaal Luc , Verrijken An , Francque Sven , Biehl Michael , Tomlinson Jeremy

Introduction: Dysregulated glucocorticoid (GC) metabolism has been implicated in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). NAFLD extends from simple steatosis, to inflammation (steatohepatitis/NASH), fibrosis and consequent cirrhosis. It is often regarded as the hepatic manifestation of the metabolic syndrome and is independently associated with increased liver and cardiovascular mortality. Changes in GC metabolism have thus far been described in small num...

ea0059oc3.6 | Obesity & diabetes | SFEBES2018

5β-reductase (AKR1D1) is a potent regulator of hepatic insulin sensitivity, carbohydrate and lipid metabolism in vitro and in vivo

Nikolaou Nikolaos , Gathercole Laura , Marchand Lea , Althari Sara , Green Charlotte , McNeil Catriona , Harris Shelley , van de Bunt Martijn , Arlt Wiebke , Hodson Leanne , Tomlinson Jeremy

Steroid hormones and BAs are established regulators of metabolic phenotype. 5β-reductase (AKR1D1) is highly expressed in the liver where it inactivates steroid hormones and catalyses a fundamental step in bile acid (BA) synthesis. We have hypothesised that AKR1D1 plays a crucial regulatory role in hepatic metabolic homeostasis. Genetic manipulation of AKR1D1 was performed in human liver HepG2 and Huh7 cells. Expression changes were confirmed by qPCR and western blotting, ...

ea0059p005 | Adrenal and steroids | SFEBES2018

A model to predict Hypothalamic-Pituitary-Adrenal (HPA) axis recovery at 6 weeks post trans sphenoidal adenomectomy: a single-centre retrospective analysis

Pofi Riccardo , Gunatilake Sonali , Isidori Andrea M , Lenzi Andrea , Macgregor Victoria , Cudlip Simon , Jafar-Mohammadi Bahram , Tomlinson Jeremy W , Pal Aparna

Background: Hypopituitarism can occur post trans-sphenoidal adenomectomy(TSA). Accurate prediction of HPA axis recovery would inform hydrocortisone replacement strategies but there are limited studies on this. In our centre, HPA axis is assessed with pre-operative short synacthen test (SST), day-8 post TSA 9am cortisol and 6-week post-TSA SST. Patients are commenced on hydrocortisone post TSA until 6 weeks review.Methods: We performed a single-centre, lo...