Searchable abstracts of presentations at key conferences in endocrinology

ea0063p60 | Adrenal and Neuroendocrine Tumours 1 | ECE2019

Adrenal morphology in a large cohort of adult subjects with congenital adrenal hyperplasia due to 21-hydroxylase deficiency

Oriolo Claudia , Gasparini Daniela Ibarra , Altieri Paola , Ruffilli Francesca , Corzani Francesca , Dalmazi Guido Di , Vicennati Valentina , Pagotto Uberto , Gambineri Alessandra

Introduction: The adrenal morphology in congenital adrenal hyperplasia (CAH) is poorly described in the literature, so that adrenal radiological evaluation is not recommended in patients with CAH. The aim of this study was to evaluate the adrenal morphology in a large cohort of adult patients with CAH due to 21-hydroxylase deficiency and its correlation with the subtype of CAH (non-classical-NCAH, simple virilizing-SV, or salt wasting-SW), the hormonal status and the treatment...

ea0063p334 | Reproductive Endocrinology 1 | ECE2019

Definition of hormonal cut-off values for discriminating polycystic ovary syndrome from non classical congenital adrenal hyperplasia due to 21-hydroxylase deficiency

Oriolo Claudia , Menabo Soara , Gasparini Daniela Ibarra , Altieri Paola , Corzani Francesca , Baldazzi Lilia , Castelli Silvia , Pagotto Uberto , Gambineri Alessandra

Objective: The aim of this study was to define which hormonal cut-off values can discriminate polycystic ovary syndrome (PCOS) from non classical congenital adrenal hyperplasia due to 21-hydroxylase deficiency (21-NCAH).Patients and methods: We included 70 women in reproductive age who attended our Unit from 2003 to 2018 for a diagnosis of PCOS with a basal 17hydroxyprogesterone (17OHP) level in the follicular phase of the menstrual cycle ≥200 ng/d...

ea0049ep669 | Obesity | ECE2017

Menopause is a major determinant of endocannabinoid 2-arachidonoylglycerol plasma level and of its relevance as biomarker of dyslipidemia and insulin resistance in lean women

Fanelli Flaminia , Mezzullo Marco , Baccini Margherita , Casadio Elena , Gasparini Daniela Ibarra , Vicennati Valentina , Pasquali Renato , Pagotto Uberto

Context: The endocannabinoid system (ECS) hypertonicity contributes to obesity development and maintenance. Excess circulating levels of ECS mediator 2-arachidonoylglycerol (2AG) were reported in obese humans, however, unstandardized experimental settings (i.e. analytical conditions, sample size, concurrent diseases/medications, statistics, gender, body mass index (BMI) and age), clouded its role in dysmetabolism and its usefulness as biomarker. The study aimed at describing 2...

ea0049ep671 | Obesity | ECE2017

Endocannabinoid 2-arachidonoylglycerol plasma level is a BMI-independent biomarker of cardiometabolic risk in adult males with higher relevance in lean and aged conditions

Fanelli Flaminia , Mezzullo Marco , Baccini Margherita , Casadio Elena , Gasparini Daniela Ibarra , Vicennati Valentina , Pasquali Renato , Pagotto Uberto

Context: Ageing and obesity are similarly characterized by metabolic impairment in terms of visceral fat, dyslipidaemia, insulin resistance and hypertension. Obesity is featured by endocannabinoid system (ECS) hyperactivation. Increased levels of endocannabinoid 2-arachidonoylglycerol (2AG) found in obesity were variously associated to dysmetabolic features, however, it is not clear whether such associations exist in normal weight conditions. Moreover, ageing contribute in mod...

ea0041oc10.5 | Reproduction & Endocrine Disruption | ECE2016

Effect of clomiphene citrate and metformin on testosterone levels in hypogonadal obese men with impaired glucose tolerance (IGT) or type 2 diabetes (DM2)

Pelusi Carla , Giagulli Vito , Baccini Margherita , Fanelli Flaminia , Mezzullo Marco , Fazzini Alessia , Casadio Elena , Prontera Olga , Pagotto Uberto , Pasquali Renato

Low testosterone (T) levels are often found in obese men with metabolic abnormalities, however the mechanism underling this condition is unclear. This study aimed to evaluate the effectiveness of clomiphene citrate (CC) in increasing T levels and improving metabolic control in hypogonadal obese men with IGT or DM2. A randomized cross-over double blind controlled study was conducted in twenty obese hypogonadal caucasian men, mean age 47 ys, classified in IGT (n=11) or ...

ea0041ep7 | Adrenal cortex (to include Cushing's) | ECE2016

Radiological formula for differentiating between secreting and non secreting adrenal adenomas

Vicennati Valentina , Casadio Elena , Pagotto Uberto , Dalmazi Guido Di , Rinaldi Eleonora , Mosconi Cristina , Papadopoulos Dimitris , Golfieri Rita , Pasquali Renato

Introduction: Purpose of this study is to find a radiological score and to correlate radiological characteristics of adrenal adenomas and functional parameters to predict subclinical glucocorticoid secretion.Methods and materials: This retrospective study included 55 patients with adenomas, investigated through CT with adrenal protocol assessing diameters, HU values of the unenhanced and contrast enhanced phases (enhanced–E, and 15 min delayed enhan...

ea0041ep658 | Female Reproduction | ECE2016

Sex hormone and steroid precursor measurement by simple and rapid liquid chromatography-tandem mass spectrometry (Lc-Ms/Ms) method: comparison with current routine immunoassays

Fanelli Flaminia , Mezzullo Marco , Fazzini Alessia , Baccini Margherita , Gambineri Alessandra , Vicennati Valentina , Pelusi Carla , Pasquali Renato , Pagotto Uberto

Though LC-MS/MS is replacing immunoassays for major glucocorticoids and androgens routine assessment, the measurement of other sex steroids and precursors is still challenging and available only in experienced research laboratories. Our aim was to develop a simple-prep and rapid 2D-LC-MS/MS method for the simultaneous measurement of estrone (E1), estradiol (E2), dihydrotestosterone (DHT) and 17OHpregnenolone.Serum volumes of 300ul were spiked with intern...

ea0037gp.02.01 | Adrenal (1) | ECE2015

Steroid profiling by liquid chromatography–tandem mass spectrometry in patients with non-secreting adrenocortical adenomas and subclinical hypercortisolism

Di Dalmazi Guido , Fanelli Flaminia , Mezzullo Marco , Casadio Elena , Rinaldi Eleonora , Garelli Silvia , Vicennati Valentina , Pagotto Uberto , Pasquali Renato

Steroid profiling by liquid chromatography–tandem mass spectrometry (LC–MS/MS) offers an accurate and simultaneous detection of a panel of steroids with several advantages over traditional assays. However, only a few studies have investigated the steroid profiling in sporadic adrenocortical adenomas. Our aim was therefore to investigate steroid secretion by LC–MS/MS in patients with non-secreting adrenocortical adenomas (NSA) and subclinical hypercortisolism (SH...

ea0037ep204 | Reproduction, endocrine disruptors and signalling | ECE2015

Salivary cortisol response to psychological stress in late adolescent and young women: impact of menstrual irregularity, hirsutism, and hyperandrogenaemia

Mezzullo Marco , Gambineri Alessandra , Fanelli Flaminia , Repaci Andrea , Prontera Olga , Di Dalmazi Guido , Pagotto Uberto , Pasquali Renato

Hyperandrogenic disorders cause psychological implication in young girls, limiting the quality of life. Salivary cortisol (SalF) testing was proved to be useful in the evaluations of acute stress responses. Aim of this study was to investigate SalF responses to a stressor event in late adolescent females. We selected 165 drug-free females aged 16–19 years from a cross-sectional epidemiological study. Saliva was collected in the morning before and after a stressor event co...

ea0035p47 | Adrenal cortex | ECE2014

Might cortisol modulate food choices in subclinical autonomous glucocorticoids hypersecretion?

Garelli Silvia , Cavazza Carla , Marcato Elisa , Rinaldi Eleonora , Dalmazi Guido di , Vicennati Valentina , Pagotto Uberto , Pasquali Renato

Introduction: The relationship between food intake and hypothalamic–pituitary–adrenal (HPA) axis has been studied in the development of visceral adiposity and obesity. The HPA axis may interact with the reward system, thus influencing food choices. On the other hand, it has been shown that macronutrients differently modulate the HPA axis activity.The aim of this study was to evaluate the dietary habits in subjects with adrenal masses (adenoma o...