Searchable abstracts of presentations at key conferences in endocrinology

ea0066p16 | Diabetes 1 | BSPED2019

Improving the rate of completion of 7 care processes at a busy district general hospital by using quality improvement methodology

Kassab Ahmed , Rawal Sanjay , Meijgaarden Birgit Van , Ahmed Shahida , Francks Ruth , White Lynn , Daniel Elizabeth , Hunt Laura

Background: The NPDA has been providing annual audit reports to paediatric units in UK from 2003 and a compliance report for 7 care processes since 2016–2017. Over the years the focus on the type of data and implications of data has changed and become more focused especially after introduction of BPT and PEER review. The paediatric diabetes services at Basildon and Thurrock University Hospitals (BTUH) were identified as negative outliers in 2016–2017 NPDA report. A Q...

ea0044p39 | Adrenal and Steroids | SFEBES2016

Salivary cortisone is a potential surrogate for serum cortisol measurement

Adam Safwaan , Minder Anna , Cottrell Elizabeth , Davies Alison , Meredith Suzanne , Preziosi Richard , Keevil Brian , Higham Claire , White Anne , Trainer Peter

Introduction: Hydrocortisone therapy in adrenal insufficiency and medical management of Cushing’s syndrome requires accurate monitoring of glucocorticoid status. Currently, this necessitates admitting patients to hospital for serial measurements of serum cortisol. From previous studies in Cushing’s, the goal of medical therapy is a mean (based on five samples) serum cortisol of 150–300 nmol/l, which is known to equate to a normal cortisol production rate. Saliva...

ea0044p177 | Obesity and Metabolism | SFEBES2016

Glucocorticoid treatment rapidly increases AgRP and food intake with delayed effects on other metabolic systems

Harno Erika , Davies Alison , Allen Tiffany-Jayne , Sefton Charlotte , Wray Jonathan R , Ramamoorthy Thanuja Gali , Coll Anthony P , White Anne

Glucocorticoids are widely prescribed therapeutic agents, however long term treatment can cause increased morbidity from adverse metabolic events, including weight gain and hyperglycaemia. The mechanisms and site of action which underpin these side-effects are not fully understood. The aim of this study was to characterise phenotypic, biochemical and neurohormonal responses in mice administered corticosterone, with a particular focus on changes seen in the early stages of chro...

ea0044p192 | Obesity and Metabolism | SFEBES2016

RNA-seq of mouse arcuate nuclei reveals pathways perturbed by glucocorticoid treatment

Wray Jonathan , Harno Erika , Davies Alison , Sefton Charlotte , Allen Tiffany-Jayne , Lam Brian Y.H. , Yeo Giles S.H. , White Anne

Glucocorticoids (GCs) are widely prescribed to treat a number of inflammatory and autoimmune conditions. However, patients receiving GCs often develop adverse metabolic effects such as hyperphagia leading to weight gain and hyperglycaemia. Little is known about the central effects of GCs; however they can act in the hypothalamic arcuate nucleus (ARC), a region involved in the integration of other energy regulatory hormones such as leptin, insulin and ghrelin. Therefore, the ai...

ea0059oc3.4 | Obesity & diabetes | SFEBES2018

Knockout of glucocorticoid receptor on AgRP/NPY/GABA (ANG) neurons identifies a potential role for this neuronal population in mediating glucocorticoid–induced insulin resistance in female mice

Harno Erika , Davies Alison , Allen Tiffany-Jayne , Sefton Charlotte , Wray Jonathan R , Coll Anthony P , White Anne

Glucocorticoids (Gcs) are used in the treatment of inflammatory disorders including asthma and rheumatoid arthritis. However, long-term use can cause metabolic side-effects including obesity and diabetes. Previous studies have shown that Gcs increase Agrp expression and that AgRP/NPY/GABA (ANG) neurons can regulate appetite and insulin sensitivity. To investigate the effects of chronic Gc treatment directly on ANG neurons, we crossed AgRP-IRES-Cre with GR...

ea0059p171 | Obesity & metabolism | SFEBES2018

Glucocorticoid-induced metabolic syndrome: establishing the role of AgRP

Sefton Charlotte , Harno Erika , Davies Alison , Allen Tiffany-Jayne , Wray Jonathan R , Coll Anthony P , White Anne

Glucocorticoid (Gc) excess, either from endogenous overproduction in disorders of the hypothalamic-pituitary-adrenal axis or exogenous medical therapy, is recognized to cause adverse metabolic side effects including obesity, hyperphagia, and hyperglycemia. The Gc receptor (GR) is widely expressed in the brain including the hypothalamus which is known to regulate energy balance. We have previously established through the administration of corticosterone (Cort) in the drinking w...

ea0038p218 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

Glucocorticoid excess increases hypothalamic AgRP and results in obesity and hyperinsulinaemia in mice

Wray Jonathan , Harno Erika , Davies Alison , Sefton Charlotte , Ramamoorthy Thanuja Gali , Allen Tiffany-Jayne , Coll Anthony P , White Anne

Glucocorticoids (Gcs) are widely prescribed to treat a number of conditions, such as arthritis and asthma. However, patients receiving Gcs often develop metabolic complications such as obesity and hyperglycaemia. The aim of this study was to investigate the molecular mechanisms in the hypothalamus which drive these adverse effects. Male C57BL/6J mice were given ad libitum access to either corticosterone (CORT; 75 μg/ml) or vehicle (V; 1% ethanol) in their drinkin...

ea0038p230 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

Maternal overnutrition programs hypothalamic neuropeptides and metabolic syndrome in offspring

Ramamoorthy Thanuja Gali , Allen Tiffany-Jayne , Davies Alison , Harno Erika , Sefton Charlotte , Wray Jonathan , Murgatroyd Christopher , White Anne

Background and objective: The prevalence of obesity is increasing worldwide and it is known that intra-uterine experience can program metabolic disorders. The hypothalamic appetite regulatory system is a key target of developmental programming by maternal nutrition. Therefore, the aim of this study was to investigate the effects of maternal overnutrition on the expression of hypothalamic genes controlling energy homeostasis.Research design: Eight week ol...

ea0038p269 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2015

Improving glycaemic control in T1DM: the Fife Insulin and Food Education for Diabetes (FIFE Diabetes) structured education programme

Boyle Luke , Jamieson Fiona , Burns Denise , Duncan Katie , Malcolm Jill , White Leslie , Chalmers John , Osborne Louise , Patterson Catherine

Background: The 2013 Scottish Diabetes Survey demonstrated that only 22% of individuals with type 1 diabetes have optimal glycaemic control, defined as a HbA1c <58 mmol/mol. The 2014 Diabetes Improvement Plan in Scotland prioritised, ‘improving the care and outcomes of all people living with type 1 diabetes’. Scotland’s Diabetes Education Advisory Group has now been established. Accordingly, the Fife Insulin and Food Education for Diabetes (FIFE Diabetes) st...

ea0028p240 | Pituitary | SFEBES2012

Transcriptional regulation of prolactin in the rat oestrous cycle

Patist Amanda , Featherstone Karen , Spiller David , Semprini Sabrina , McNeilly Judith , McNeilly Alan , Mullins John , White Michael , Davis Julian

Circulating levels of prolactin are subject to acute and long-term regulation by many factors including oestrogen and dopamine. We have studied the regulation of prolactin promoter activity in living pituitary cells using transgenic Fischer rats in which reporter gene expression is regulated by the human prolactin gene locus (hPRL-d2EGFP). We have previously identified pulsatile prolactin transcription patterns in living lactotroph cells in fetal tissue, that became stabilised...