Searchable abstracts of presentations at key conferences in endocrinology

ea0029p1127 | Neuroendocrinology | ICEECE2012

Regional distributions of kisspeptin and gonadotropin-inhibiting hormone peptides in ovine and rat brains

Hasegawa Y. , Hashimoto O. , Tsutsui K.

The reproductive system is controlled by (GnRH) secretion from the brain, which is finely modulated by a number of factors, including gonadal sex steroids and inhibins. It was recently claimed that kisspeptin (KP) and gonadotropin inhibitory hormone (GnIH) neurons are regulators of GnRH secretion. Therefore, we have developed highly specific and sensitive fluoro-immunoassays (FIAs) using Eu-labeled antigens for KP 10 and GnIH. In this study, the distributions of KP 10 and GnIH...

ea0037ep398 | Diabetes (pathiophysiology & epitemiology) | ECE2015

Modulation of metabolic parameters and antioxidant enzymes in diabetic ageing female rat brains: beneficial role of metformin

Kumar Pardeep , Kale R K , Baquer N Z

Objective: The objective of this study was to investigate beneficial effects of metformin on membrane bound enzymes (monoamine oxidase, Na+K+ ATPse) and antioxidant enzymes (sueroxidase dismutase and glutathione S-transferases), lipid peroxidation, neurolipofuscin, and DNA degradation in diabetic aging female rats.Methods: Young (3 months) adult (12 months) and aged (24 months) rats will be diabetic by using alloxan monohyd...

ea0032p503 | Endocrine disruptors | ECE2013

Protective potential of 17-beta-estradiol on membrane linked functions in aging female rats: a behavioral, biochemical and ultrastructural study

Kumar Pardeep , Kale R K , Baquer N Z

Background: Alzheimer’s disease (AD) is the most common form of dementia in the elderly. AD is characterized by the presence of amyloid plaques which are formed from deposits of b-amyloid protein (Ab). These changes increase during menopausal condition in females when the level of estradiol is decreased.Objective: The objective of this study was to investigate neuroprotective potential of 17β estradiol (E2) treatment on the activities of acetyl...

ea0013p129 | Growth and development | SFEBES2007

Pituitary tumor transforming gene (PTTG), separase and Rad21 in neural cell growth

Pemberton Helen , Franklyn Jayne , Boelaert Kristien , Chan Shiao , Kim Caroline , Cheng Sheue-Yann , Kilby Mark , Mccabe Christopher

The key mitotic regulator pituitary tumor transforming gene (PTTG) is expressed at low levels in fetal brain compared with adult, and modulates the proliferation of human embryonic neuronal N-Tera2 (NT2) cells. We examined the function and expression of PTTG’s interacting partner separase, along with Rad21, the functional component of cohesin, which is cleaved by separase following interaction with PTTG. In contrast to PTTG, the cleaved forms of separase and Rad21 were hi...

ea0003p186 | Neuroendocrinology | BES2002

The pattern of green fluorescent protein (GFP) expression following intrahypothalamic injection of adeno-associated virus-GFP

Ward H , Gardiner J , Kong W , Murphy K , Bloom S

Adeno-associated virus (AAV) has been used to express GFP in a variety of tissues. AAV has a tropism for neurones and we have been using AAV-GFP to determine the anatomical localisation of gene transfer after injection into the hypothalami of Wistar rats. The long term aim is to use this viral vector as a tool for investigating the hypothalamic control of appetite, energy expenditure and pituitary function.Male Wistar rats were anaesthetised and injecte...

ea0029p1074 | Neuroendocrinology | ICEECE2012

Mechanisms for the protective effects of 17-β-estradiol: relevance to depressive symptoms in Parkinson’s disease

Kumar P. , Kale R. , Cowsik S. , Baquer N.

Background: Parkinson’s disease (PD) is a neurodegenerative disease and a movement disorder characterized by loss of dopaminergic neurons in the substantia nigra causing dopamine depletion in the striatum. Neurodegeneration in PD occurs due to multiple pathways including oxidative stress, mitochondrial damage, protein aggregation. These changes increase during menopausal condition in females when the level of estradiol is decreased. Recently, there has been a growing inte...

ea0086ce1.3 | Endocrinology in a Warming Dirty World | SFEBES2022

Environmental endocrine disruption of brain and behavior across generations

Gore Andrea , Gillette Ross , Thompson Lindsay

Environmental endocrine-disrupting chemicals (EDCs) are exogenous chemicals that perturb hormones and their actions. In our laboratory, we are studying how early life exposures to EDCs affect the developing brain and lead to behavioral dysfunctions in exposed individuals and their descendants across generations. To do this, pregnant rats are fed one of three treatments: the weakly estrogenic commercial polychlorinated biphenyl (PCB) mixture, Aroclor 1221 (A1221, 1 mg/kg); vinc...

ea0070pl7 | Effects of EDCs on neuro-endocrine systems and behaviour | ECE2020

Endocrine disruption of neuroendocrine development, function, and behavior

Gore Andrea

Environmental endocrine-disrupting chemicals (EDCs) are exogenous chemicals that perturb hormones and their actions. Exposures to EDCs during critical periods of life, especially in the developing fetus and infant, are particularly problematic due to the high sensitivity of the perinatal hypothalamus to endogenous hormones and exogenous compounds. Our lab has been using a rat model of prenatal exposure to two classes of EDCs: a polychlorinated biphenyl (PCB) mixture used previ...

ea0014s5.1 | Novel bioactive peptides – lessons from animals | ECE2007

Discovery of novel bioactive peptides: the uniquely important contribution of amphibians to mammalian neuropeptidology

Vaudry Hubert , Tostivint Hervé , Lihrmann Isabelle , Chartrel Nicolas , Fournier Alain , Leprince Jérôme , Tonon Marie-Christine , Conlon J. Michael

The concentration of many neuropeptides in the brains of ectothermic vertebrates is several orders of magnitude higher than in the brains of mammals. We have taken advantage of this singular situation to isolate from the brain of the European green frog, Rana esculenta, a number of regulatory peptides that are orthologous to mammalian neuroendocrine peptides. These include α-MSH, γ-MSH, two tachykinins, two GnRH variants, CRH, PACAP, NPY, CGRP, CNP, GRP, and O...

ea0012p69 | Neuroendocrinology and behaviour | SFE2006

Quinelorane activates oxytocin neurones in the hypothalamus during penile erection

Baskerville TA , Douglas AJ

Central dopamine and oxytocin have been shown in many studies to facilitate aspects of male sexual function. However, it is poorly understood how these two neuromodulators interact to mediate sexual behaviour or which receptors are involved. Much evidence has recently implicated a role for the dopamine D2 receptor in the paraventricular nucleus (PVN) and supraoptic nucleus (SON) in regulating aspects of male sexual behaviour. Using Sprague-Dawley male rats (225–250 g), we...