Searchable abstracts of presentations at key conferences in endocrinology

ea0032p33 | Adrenal cortex | ECE2013

Salivary cortisol is a useful tool to assess the immediate response to pasireotide in patients with Cushing’s disease

Cardinaletti Marina , Trementino Laura , Concettoni Carolina , Marcelli Giorgia , Polenta Barbara , Spinello Maurizio , Boscaro Marco , Arnaldi Giorgio

Introduction: Pasireotide is a promising treatment option for patients with Cushing’s disease (CD). The measurement of salivary cortisol is useful for diagnosing hypercortisolism and monitoring patients with CD following pituitary surgery. It may also be a better index of cortisol secretion than serum cortisol or urinary free cortisol (UFC). We investigated the value of salivary cortisol in monitoring short-term efficacy of pasireotide in patients with CD.<p class="ab...

ea0032p45 | Adrenal cortex | ECE2013

Long-term efficacy of pasireotide in a patient with Cushing's disease and diabetes: results in the short term are not always predictive of long-term response

Trementino Laura , Cardinaletti Marina , Concettoni Carolina , Marcelli Giorgia , Boscaro Marco , Arnaldi Giorgio

The management of Cushing’s disease (CD) can be problematic, particularly when the disease persists following pituitary surgery. Here we report the case of a 55-year old woman with CD that persisted after repeat transsphenoidal surgery. The patient had an overt phenotype with facial rubeosis, central obesity with supraclavicular fat accumulation, cervical fat pad and proximal muscle weakness. She had received anti-hypertensive drugs and insulin for diabetes mellitus. Horm...

ea0020p580 | Neuroendocrinology, Pituitary and Behaviour | ECE2009

Human corticotropin releasing hormone (hCRH) test performance in the differential diagnosis between Cushing's disease and pseudo-Cushing state is enhanced by combined ACTH and cortisol analysis

Tirabassi Giacomo , Arnaldi Giorgio , Papa Roberta , Furlani Giorgio , Trementino Laura , Cardinaletti Marina , Faloia Emanuela , Boscaro Marco

Objective: The corticotropin releasing hormone (CRH) test does not reliably distinguish between Cushing’s disease (CD) and normality or pseudo-Cushing state (PC). In this study we assessed whether the application of novel criteria could enhance its diagnostic performance.Design: Retrospective study.Patients: Fifty-one subjects with CD, 26 with PC and 31 control subjects (CT).Measurements: All subjects un...

ea0016p12 | Adrenal | ECE2008

RNA interference inhibits VEGF and EG-VEGF expression of H295R human adrenocortical carcinoma cell line

Appolloni Gloria , Arnaldi Giorgio , Di Stefano Michele , Spegni Monica , Cardinaletti Marina , Trementino Laura , Boscaro Marco

Background: The development of a vascular supply is a critical factor in the growth and metastatic spread of malignant tumors. The recognition of the vascular endothelial growth factor (VEGF) pathway as a key regulator of angiogenesis has led to a considerable efforts to exploit its potential for therapy in oncology. Endocrine gland-derived (EG-VEGF) has been recently identified as an endothelial cell mitogen, predominantly expressed in steroidogenic tissues; it represents a n...

ea0016p26 | Adrenal | ECE2008

Glucocorticoid receptor gene polymorphisms and bone mineral density in hpa axis disorders

Arnaldi Giorgio , Appolloni Gloria , Romagni Paola , Cardinaletti Marina , Trementino Laura , Polenta Barbara , Concettoni Carolina , Boscaro Marco

Glucocorticoid receptor (GR) gene polymorphisms have been associated with interindividual variations in glucocorticoid sensitivity in healthy individuals.Aim: Aim of this study was to investigate whether there was an association with sensitivity to glucocorticoids and bone mineral density in HPA axis disorders.Methods: We analyzed 3 polymorphisms of the GR gene (Bcl1, N363S, ER22/23EK) in 185 subjects: 52 patients with Cushing&#146...

ea0016p125 | Clinical cases | ECE2008

Effects of gender on clinical and biochemical features in Cushing's disease

Cardinaletti Marina , Trementino Laura , Polenta Barbara , Concettoni Carolina , Mancini Tatiana , Boscaro Marco , Arnaldi Giorgio

Cushing’s disease (CD) occur more frequently in females than in males, but in men this disease seems appear at a younger age and with more severe symptoms and clinical course. Aim of this study was to compare clinical features and biochemical indices of hypercortisolism in male and female patients with CD.Methods: Forty-five patients (35 females and 10 males) with confirmed CD were analyzed. All patients underwent a complete biochemical and endocrin...

ea0011p380 | Diabetes, metabolism and cardiovascular | ECE2006

Impaired glucose metabolism in patients with Cushing’s Syndrome

Arnaldi GA , Mancini TM , Polenta BP , Cardinaletti MC , Canibus PC , Faloia EF , Boscaro MB

Glucose metabolism was investigated in 65 patients with Cushing’s syndrome (CS), 19 with cortisol-secreting adrenal adenoma (mean age 47.2±4.9), 40 with ACTH secreting pituitary adenoma (mean age 41.4±12.48), 4 with cortisol secreting adrenal cancer (mean age 63.0±8.4) and 2 patients with ectopic ACTH secretion (38.7±11.9).Eleven patients (17%) were diagnosed as diabetic after abnormal fasting glucose levels (>126 mg/dl). Whi...

ea0037ep88 | Adrenal cortex | ECE2015

A single-centre 10-years experience with pasireotide in Cushing's disease: patients characteristics and outcome

Trementino Laura , Michetti Grazia , Angeletti Alessia , Marcelli Giorgia , Concettoni Carolina , Cardinaletti Marina , Polenta Barbara , Boscaro Marco , Arnaldi Giorgio

Introduction: Pasireotide is the first pituitary-directed drug approved for Cushing’s disease (CD). We report our 10-years experience with pasireotide in CD reviewing and analysing data about all the patients treated with pasireotide at our referral centre both in randomised trials and in clinical practice.Patients and methods: Twenty active CD patients were treated. Fourteen patients were treated with pasireotide in randomised trials and six patien...

ea0011p533 | Endocrine tumours and neoplasia | ECE2006

N363S and BclI variants in the glucocorticoid receptor gene and their associations in Cushing’s syndrome and Addison’s disease

Giorgio Arnaldi GA , Gloria Appolloni GA , Claudia Polloni CP , Paola Romagni PR , Barbara Polenta BP , Marina Cardinaletti MC , Alberto Falorni AF , Marco Boscaro MB

Objective: Interindividual variation in glucocorticoid sensitivity can be partly explained by polymorphisms in the GC receptor gene. The most frequent polymorphisms of the GR gene (N363S and BclI) are associated with increased BMI, less lean mass, higher cholesterol and insulin levels. In the present study we investigated the role of the N363S and the BclI polymorphisms in patients with adrenal disease to evaluate their prevalence and the possible clinic and hormone correlatio...