Searchable abstracts of presentations at key conferences in endocrinology

ea0014oc5.4 | Thyroid basic | ECE2007

BRAFV600E mutations but not RET/PTC rearrangements are correlated with a lower expression of NIS mRNA expression in papillary thyroid cancer (PTC)

Romei Cristina , Piampiani Pamela , Faviana Pinuccia , Basolo Fulvio , Bottici Valeria , Sculli Mariangela , Berti Piero , Materazzi Gabriele , Pinchera Aldo , Elisei Rossella

Several studies have identified a relationship between oncogene activation and dedifferentiation of PTC. Mutations of RAS, RET/PTC and BRAF modulate the expression of thyroid genes. An impaired NIS expression has been demonstrated in PTCs harboring the BRAFV600E mutation.Aim of this study was to analyze BRAF and RET/PTC1-3 alterations and their influence on the expression of thyroid differentiation genes. Seventy-one PTC samples were studied. Quantitative analysis o...

ea0011p860 | Thyroid | ECE2006

Clinical parameters and echographic patterns are poorly predictive of malignancy in thyroid nodules with a cytological diagnosis of follicular and Hv¨rthle cell neoplasia

Rago T , Di Coscio G , Basolo F , Scutari M , Berti P , Romani R , Faviana P , Miccoli P , Pinchera A , Vitti P

Aim of the study was to evaluate whether clinical parameters and echographic patterns may be predictive of malignancy in thyroid nodules with a cytological diagnosis of follicular (FOL) and H?rthle cell (HC) neoplasia. The study included 495 patients (388 F: mean age 45±14 yr; 107 M: mean age 46±14 yr) with FOL (n=418) and CH (n=77) nodules, ‘cold’ at scintiscan and undergone to thyroidectomy. Clinical parameters considered were: sex, age, goi...

ea0011p868 | Thyroid | ECE2006

Clinical parameters and echographic patterns are poor predictive of malignancy in follicular and Hürthle cell nodules at cytology

Rago T , Di Coscio G , Basolo F , Scutari M , Berti P , Romani R , Faviana P , Miccoli P , Pinchera A , Vitti P

Aim of the study was to establish the diagnostic value of nuclear atypia thyroid nodules with cytological diagnosis of follicular (FOL) and Hv¨rthle cell (HC) neoplasia. 1039/23.313 fine needle aspiration (FNA) performed in January 2002 June 2005 in the Department of Endocrinology, University of Pisa had cytological diagnosis of FOL e CH. FNA was performed under echo guidance, using 23 gauge needle attached to 10 mL syringe. The material was air-dryed and stained with Pap...

ea0011p870 | Thyroid | ECE2006

Identification of a novel germ-line point mutation of the ret gene (Met848Thr) in a patient affected by medullary thyroid carcinoma and castleman’s syndrome

Cosci B , Altea M , Castagna M , Romei C , Piampiani P , Vivaldi A , Ciampi R , Faviana P , Basolo F , Pinchera A , Elisei R

Mutations in the RET proto-oncogene are responsible for multiple endocrine neoplasia type II. Somatic RET mutations were described in 50% of medullary thyroid carcinoma (MTC). We describe here a novel germline mutation of the RET gene detected in an apparentely sporadic MTC.The index case was a 67 years old patient who arrived at our observation for a bilateral laterocervical linfoadenopathy. The patient was clinically evaluated for thyroid function and ...

ea0014p130 | (1) | ECE2007

Enhanced expression of functional P2X7 receptor in human papillary thyroid cancer

Dardano Angela , Ferrari Davide , Cuccato Sabina , Santini Eleonora , Caraccio Nadia , Gulinelli Sara , Callegari Giulia , Faviana Pinuccia , Solini Anna , Di Virgilio Francesco , Monzani Fabio

Extracellular ATP modulates several biological processes via activation of plasma membrane receptors (P2Rs) in normal human thyrocytes (NT). We characterized P2Rs expression and function in two thyroid cancer cell lines: FB1 (anaplastic cancer) and FB2 [papillary cancer (PTC)]. P2Rs expression was evaluated by RT-PCR and WB, intracellular Ca2+changes by fluorimetric technique (Fura2-AM), IL-6 release by ELISA, intracellular [i(ATP)] and extracellular ATP [...