Searchable abstracts of presentations at key conferences in endocrinology

ea0016p662 | Signal transduction | ECE2008

Corticotropin releasing hormone receptor signalling pathways and regulation of NOSIII activity in syncytialized BeWo cells

Delidaki Maria , Gu Mei , Vatish Manu , Grammatopoulos Dimitris

Placental corticotrophin releasing hormone (CRH) appears to play important physiological roles during pregnancy and labour. In the placenta, CRH appears to regulate diverse actions such as human trophoblast cell grown and invasion, tissue remodeling, direct modulation of the placental prostaglandin generation and bioavailability and control of placental vascular tone through regulation of endothelial NOS (NOSIII) expression and activation1. CRH actions are mediated ...

ea0016s19.1 | Hormone receptors and adipose tissue | ECE2008

The family of corticotropin-releasing hormone (CRH) peptides: important regulators of adipocyte function

Grammatopoulos Dimitris

Through activation of the HPA axis, corticotropin-releasing hormone (CRH), the 41-amino acid hypothalamic peptide, plays a fundamental role in mammalian survival and response to stressful stimuli. CRH belongs to a family of stress-related peptides that includes urocortins (UCNs) that have been linked with many pathophysiological effects. Their actions are mediated via activation of two types of CRH receptors (CRH-R1 and R2) that exhibit distinct pharmacology and functional pro...

ea0011oc2 | Signal transduction OC1 Novartis Oncology Young Investigator Award | ECE2006

Signalling and internalisation characteristics of corticotropin-releasing hormone (CRH) receptors

Markovic D , Grammatopoulos D

Corticotropin releasing hormone (CRH), and the urocortins (UCN) play key roles in mammalian pathophysiology. Their actions are mediated through two types of G-protein coupled CRH receptors, (R1 and R2) which exhibit distinct pharmacological characteristics and determine specificity of CRH and UCN actions. Several CRH-R1 mRNA splice variants have been identified in native tissues, encoding receptor isoforms with various aminoacid inserts or deletions, termed R1β, R1c, R1d ...

ea0029p481 | Developmental endocrinology | ICEECE2012

Regulation of trophoblast 11β-hydroxysteroid dehydrogenase type 2 expression and role of corticotropin-releasing hormone

Hein A. , Vatish M. , Grammatopoulos D.

The formation of the placental syncytiotrophoblast through trophoblast differentiation is a key cellular event, essential for successful embryonic implantation, oxygen/nutrient transport and secretion of placental hormones necessary for fetal development. Trophoblast differentiation alters expression of various molecules with important roles in placental biology, including 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), the major component of the placental glucoc...

ea0014oc9.4 | Signal transduction | ECE2007

Signalling and internalisation properties of corticotrophin-releasing hormone (CRH) receptor type 2

Markovic Danijela , Lehnert Hendrik , Grammatopoulos Dimitris

The family of urocortins (UCNs) exert important pathophysiological actions in the control of peripheral homeostatic mechanisms, through activation of the type 2-corticotropin releasing hormone receptor (CRH-R2). This G-protein coupled receptor preferentially binds urocortins (UCN, UCNII and UCNIII) than CRH. In most tissues, CRH-R2 activation leads to increased cAMP production. In this study we used HEK293 cells stably overexpressing recombinant CRH-R2β receptors to inves...

ea0028p218 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2012

Unique roles of CRH-Rs controlling adipocyte biology and transdifferentiation

Lu Buyu , Markovic D. , Pessin Jeff , Lehnert Hendric , Grammatopoulos Dimitris

In addition to well established role in coordinating mammalian adaptive responses to stressful stimuli, the family of “stress” peptides that includes corticotropin releasing hormone (CRH) and urocortins (Ucns) are emerging as important regulators of the homeostatic mechanisms regulating energy balance and metabolism. CRH acting through specific G-protein coupled receptors, CRH-R1 and R2 can target multiple peripheral tissues such as skeletal muscle and adipose tissue...

ea0016p664 | Signal transduction | ECE2008

cAMP/PKA and ERK1/2 dependent feedback mechanisms regulating type 2 CRH receptor signalling

Markovic Danijela , Punn Anu , Lehnert Hendrik , Grammatopoulos Dimitris

Corticotropin-releasing hormone (CRH) and its related peptides, the urocortins (UCNs) mediate their effects by binding to two types of GPCRs, CRH-R1 and CRH-R2. In most target tissues, the adenylyl cyclase/cAMP/PKA and the mitogen activated protein kinase (MAPK) are the two main pathways mediating the biological effects of CRH-Rs. For most multi-signal G-protein coupled receptors (GPCRs), there is considerable level of ‘cross-talk’ between different signalling cascad...

ea0016p665 | Signal transduction | ECE2008

Signalling properties of corticotropin-releasing hormone receptor (CRH-R1/2) in adrenal (H295R) cells

Punn Anu , Ramanjaneya Manjunath , Lehnert Hendrik , Grammatopoulos Dimitris

Corticotropin-releasing hormone (CRH) and urocortins (UCN) bind to two types of GPCRs (CRH-R1 and CRH-R2), activating a plethora of signalling cascades, including the mitogen-activated protein kinases (MAPKs) pathways. Although previous studies have identified that CRH directly regulates adrenal steroidogenesis1, the second messenger systems and downstream effectors remain poorly defined. The adrenal cell line H295R, which express both CRH-Rs1 was used to...

ea0006p70 | Steroids | SFE2003

Effects of food deprivation on the expression and signalling of orexin and orexin receptors in the rat hypothalamus and adrenal gland

Karteris E , Machado R , Grammatopoulos D , Hillhouse E , Randeva H

Orexins, in addition to regulating food intake and playing a role in the sleep wake cycle, are involved in the activation of HPA axis. Starvation induces metabolic changes in the body, including upregulation of orexin and its receptors in the hypothalamus. We investigated the expression and signalling characteristics of rat orexin receptors at the hypothalamic and adrenal level in food deprived animals and controls. Food deprivation induced both orexin type-1 (OX1R) and type-2...

ea0050p336 | Obesity and Metabolism | SFEBES2017

Metabolic phenotype of Male Obesity-associated Secondary Hypogonadism pre- and post-replacement therapy with intra-muscular Testosterone undecanoate therapy

Dimitriadis Georgios K , Randeva Harpal S , Grammatopoulos Dimitris K , Barber Thomas M

Aim: To explore the metabolic phenotype of Male Obesity-associated Secondary Hypogonadism (MOSH) and following treatment with long-acting intramuscular (IM) testosterone undecanoate.Methods: A prospective observational pilot study on metabolic effects of IM testosterone undecanoate in MOSH (Hypogonadal [HG] group, n=13), including baseline comparisons with controls (Eugonadal [EG] group, n=15). Half the subj...