Searchable abstracts of presentations at key conferences in endocrinology

ea0015p148 | Diabetes, metabolism and cardiovascular | SFEBES2008

Thyrotropin receptor activation and preadipocyte biology; dissecting the effects of Gsα and Gβγ signalling on 3T3-L1 preadipocytes

Zhang Lei , Paddon Carol , Grennan-Jones Fiona , Ludgate Marian

TSH receptor (TSHR) expression increases >100-fold during adipogenesis and signals via CREB. Since CREB activation has been reported to be ‘necessary and sufficient to induce adipogenesis’, we investigated whether TSHR activation, a feature of most thyroid dysfunction, has a role in adipocyte biology and evaluated the contribution of Gsα and Gβγ signalling.Retroviral vectors for WT or constitutively active mutant TSHR* or ...

ea0011p862 | Thyroid | ECE2006

Comparison of the effects of thyrotropin receptor (TSHR) activation on preadipocyte cell lines from white (WAT) and brown (BAT) adipose tissue

Zhang L , Paddon C , Baker GC , Ludgate M

Adipogenesis is induced when intracellular cAMP increases and follows cell cycle arrest. During preadipocyte differentiation expression of the TSHR, which signals via cAMP and the inositol phosphate cascade, is upregulated. To investigate the role of TSHR activation in adipogenesis, we have developed an in vitro model in which constitutively active TSHR mutants (L629F, M453T) are introduced by retroviral vectors (RV).We aimed to compare the effect...

ea0019oc33 | Thyroid, reproduction and endocrine tumours | SFEBES2009

Thyrotropin receptor (TSHR) activation increases hyaluronan (HA) production in preadipocyte-fibroblasts; explanation for HA accumulation in thyroid dysfunction?

Zhang L , Bowen T , Paddon C , Webber J , Giles P , Steadman R , Ludgate M

The thyrotropin receptor (TSHR) is expressed in the thyroid and in precursors undergoing lineage-specific differentiation, e.g adipogenesis. The TSHR is activated by TSH, thyroid stimulating antibodies (TSAB) and gain-of-function mutations (TSHR*). To investigate the role of extra-thyroidal TSHR activation we compared the gene-expression profiles of non-modified human subcutaneous preadipocytes with the parallel TSHR* population. Twenty-seven genes were significantly upregulat...