Searchable abstracts of presentations at key conferences in endocrinology

ea0067o53 | Oral Presentations | EYES2019

Seasonal changes of serum gonadotropins and testosterone in men: a reappraisal from a large data set of real-world observations over eight years

Spaggiari Giorgia , Granata Antonio RM , Setti Monica , Tagliavini Simonetta , Trenti Tommaso , Simoni Manuela , Santi Daniele

Objective: Environmental rhythmicity seems able to affect the hypothalamic-pituitary-gonadal axis in animals, but the extent of its influence in humans is still controversial. This study was designed to evaluate seasonal fluctuations of the main hormones involved in the hypothalamic-pituitary-gonadal axis in men, using a big data approach.Methods: An observational, retrospective, big data trial was carried out, including all testosterone, luteinizing hor...

ea0041gp176 | Receptors & Signalling | ECE2016

Luteinizing hormone and human chorionic gonadotropin (hCG) action on the same receptor results in different in vitro intracellular signaling in mouse primary Leydig cells

De Pascali Francesco , Riccetti Laura , Poti Francesco , Tagliavini Simonetta , Trenti Tommaso , Simoni Manuela , Casarini Livio

Luteinizing hormone (LH) and human chorionic gonadotropin (hCG) are two glycoprotein hormones regulating development and reproduction. Despite binding the same receptor (LHCGR), they elicit different intracellular signaling. In vitro non-equivalence of hCG and LH was previously demonstrated in human primary granulosa cells. The aim of this study is to compare the effects of LH and hCG in mouse primary Leydig cells in vitro, naturally expressing the murine rec...

ea0035p525 | Endocrine tumours and neoplasia | ECE2014

The presence of B-RAF V600E and K601E mutations in our Ligurian population

Eleonora Monti , Lorenzo Mortara , Simonetta Zupo , Gianluca Ansaldo , Mauro Truni , Francesco Minuto , Massimo Giusti

Introduction: Differentiated thyroid carcinoma (DTC) is the most common endocrine neoplasm and its rate it’s constantly increasing. Papillary thyroid carcinoma (PTC) represents 87% of all DTC and its incidence is raising at 89%. The thyroid carcinoma preoperative diagnosis consists in fine-needle ago aspiration (FNAB). However in the diagnostic cytology there actually is a ‘grey zone’, the ‘follicular lesion of indeterminate significant or suspicious for fo...

ea0070aep806 | Reproductive and Developmental Endocrinology | ECE2020

Seasonal changes of serum gonadotropins and testosterone in men revealed by a large data set of real-world observations over nine years

Spaggiari Giorgia , Raffaele Michele Granata Antonio , Setti Monica , Tagliavini Simonetta , Trenti Tommaso , Simoni Manuela , Santi Daniele

Background: Environmental rhythmicity seems able to affect the hypothalamic-pituitary-gonadal axis in animals to achieve reproductive advantages. However, conflicting results were obtained when assessing the environmental-dependent rhythmicity on reproductive hormone secretion in humans.Aim of the study: This study was designed to evaluate seasonal fluctuations of the main hormones involved in the hypothalamic-pituitary-gonadal axis in men, using a big d...

ea0022p702 | Obesity | ECE2010

Acylated, unacylated ghrelin and obestatin regulation during oral glucose tolerance test in obese prepubertal and pubertal children

Prodam Flavia , Bellone Simonetta , Petri Antonella , Trovato Letizia , Genoni Giulia , Dondi Elena , Demarchi Irene , Bona Gianni

Physiological role of acylated ghrelin (AG), unacylated ghrelin (UAG) and obestatin is poorly understood especially in childhood. To understand their biological implications in obesity and metabolic syndrome (MS), we measured AG, UAG, obestatin, at fasting and every 60′ during oral glucose tolerance test (OGTT) in 60 prepubertal (PP–OB; 32) and pubertal (P-OB; 28) children. Paediatric IDF 2007 criteria was used to identifying MS. In PP-OB group, 20 (62.5%) children ...

ea0014oc5.2 | Thyroid basic | ECE2007

Tyroglobulin (Tg) depletion in receptor associated protein (RAP) KO mice is due to a reduction of Tg aggregates

Lisi Simonetta , Botta Roberta , Pinchera Aldo , Marcocci Claudio , Marinò Michele

RAP KO mice have a reduction of colloidal Tg resulting in subclinical hypothyroidism and histological signs of goiter. The difference in colloidal Tg between RAP KO and WT mice was striking by immunohistochemistry, but could not be detected in thyroid extracts. To explain this discrepancy, we hypothesized that the reduction of Tg reflected a reduction of Tg aggregates discarded during tissue extraction. To investigate this possibility, pellets obtained by thyroid homogenizatio...

ea0056p942 | Female Reproduction | ECE2018

Recombinant FSH and biosimilars result in different intracellular signaling

Riccetti Laura , Sperduti Samantha , Lazzaretti Clara , Tagliavini Simonetta , Simoni Manuela , Casarini Livio

Introduction: Follicle-stimulating hormone (FSH) biosimilars, differing for source cell and glycosylation pattern, are commercially available for therapy of hypogonadism and assisted reproduction techniques (ART). Although these molecules are commonly used in clinical practice, comparison of their action in vitro was poorly investigated.Aim: The aim of the study is to compare recombinant FSH- and biosimilars-induced cell response in vitro</e...

ea0073aep201 | Diabetes, Obesity, Metabolism and Nutrition | ECE2021

Liraglutide for weight management in syndromic obesity: effects in overgrowth adult Beckwith–Wiedemann syndrome

Caputo Marina , Daffara Tommaso , Bellone Simonetta , Mancioppi Valentina , Marzullo Paolo , Aimaretti Gianluca , Prodam Flavia

ContextGenetic obesity, including syndromic and non-syndromic forms, represent a minority of cases but gene dysregulations make their management particularly difficult. Among them, Beckwith–Wiedemann syndrome (BWS) is a multisystem human genomic imprinting disorder characterized by overgrowth, macroglossia, abdominal wall defects, hemi hyperplasia, enlarged abdominal organs, and an increased risk of embryonal tumours. The syndrome is caused by genet...

ea0092ps2-18-03 | Treatment 1 | ETA2023

A systematic drug repurposing approach for anaplastic thyroid cancer

Manzotti Gloria , Torricelli Federica , Manicardi Veronica , Faria do Valle Italo , Piana Simonetta , Ciarrocchi Alessia

Anaplastic thyroid cancer (ATC) is the most aggressive and lethal form of thyroid cancer. ATC rarely develop as ex-novo lesions, but rather evolves from pre-existing well-differentiated thyroid cancer (DTC) through a de-differentiation process that leads cells to acquire an aggressive phenotype. ATC are refractory to radiotherapy, having lost the expression of Sodium-Iodine Symporter, and often also to standard chemotherapy. Moreover, debulking surgery is frequently difficult,...

ea0049gp51 | Bone & Calcium Homeostasis 2 | ECE2017

Calcium to phosphorous ratio (Ca/P) as helpful index to recognize primary hyperparathyroidism, but not primary hypoparathyroidism: a big-data approach

Vincentis Sara De , Santi Daniele , Rochira Vincenzo , Setti Monica , Tagliavini Simonetta , Varani Manuela , Trenti Tommaso , Simoni Manuela , Madeo Bruno

Background: Primary hyperparathyroidism (HyperPT) and primary hypoparathyroidism (HypoPT) are often underdiagnosed. Several strategies have been investigated in the past in order to identify diagnostic parameters, although the diagnosis of both HyperPT and HypoPT remains challenging so far, especially in asymptomatic patients. Calcium (Ca) and phosphorus (P) are inversely related together, thus the Ca/P ratio could be an useful tool to define these conditions. Recently, we pro...