Searchable abstracts of presentations at key conferences in endocrinology

ea0011p608 | Neuroendocrinology and behaviour | ECE2006

Bone mass and metabolism in active acromegaly – no impact of gonadal status. a study of 73 patients

Fougner SL , Godang K , Ueland T , Schreiner T , Bollerslev J

Background: Growth hormone (GH) is known to have stimulatory effects on bone tissue through a complex interaction of GH, IGF-1 and IGFBPs. The gonadal status is also thought to influence bone metabolism. Bone mass in patients with acromegaly reflects the long-time effects of GH excess on bone. However, data on bone status in acromegaly have been conflicting.Objective: The object of the study was to examine the impact of chronic GH excess on bone mass and...

ea0081p14 | Adrenal and Cardiovascular Endocrinology | ECE2022

Profound changes of inflammatory and cardiovascular biomarkers in patients with autonomous cortisol secretion and Cushing syndrome

Astrom Ueland Grethe , Methlie Paal , Lovas Kristian , Saevik Ase Bjorvatn , Heie Anette , Sverre Husebye Eystein

Objective: Determine inflammatory and cardiometabolic biomarkers in patients with autonomous cortisol secretion (ACS), compared with healthy controls and patients with overt Cushing syndrome (CS).Method: Serum from prospectively included patients with ACS (n=65), overt CS (n=8), and healthy subjects (n=120) were analysed for 92 different inflammatory biomarkers using proximity extension assay.Results: ACS...

ea0049ep49 | Adrenal cortex (to include Cushing's) | ECE2017

The short Synacthen test revisited – reevaluation of the normal reference range using LCMSMS

Ueland Grethe AEstrom , Methlie Paal , Oksnes Marianne , Lovas Kristian , Thordarson Hrafnkell , Husebye Eystein

Background: The Synachten test is used to diagnose for adrenal insufficiency (AI) and non-classical congenital adrenal hyperplasia (CAH). The cut-off levels for s-cortisol and s-17-hydroxyprogesterone are derived from immunoassays that were not well standardized and are no longer in use. Introduction of liquid chromatography tandem mass spectrometry (LCMSMS) could resolve the lack of standardization of steroid hormone assays and enable increased diagnostic accuracy.<p clas...

ea0041gp24 | Adrenal (2) | ECE2016

Does measurement of serum dexamethasone increase diagnostic accuracy of the overnight dexamethasone-suppression test?

Ueland Grethe AEstrom , Methlie Paal , Thordasron Hrafnkell Baldur , Lovas Kristian , Kelp Oskar , Mellgren Gunnar , Husebye Eystein

Background: 1-mg overnight dexamethasone-suppression test (DST) is commonly used to screen for hypercortisolism. Sensitivity is high (95%), but specificity is low (80%), leading to false positive results. Identifying individuals with abnormal dexamethasone absorption or metabolism could enhance diagnostic accuracy.Aim: Use serum dexamethasone (s-DXT) to increase diagnostic accuracy of DST.Methods: Prospective study of DST for clini...

ea0035oc8.1 | Pituitary clinical | ECE2014

Metabolic improvement after treatment of acromeglay is determined by the increase of gynoid fat mass and the decrease of serum vaspin and IGF1

Olarescu Nicoleta C. , Heck Ansgar , Godang Kristin , Ueland Thor , Bollerslev Jens

Context: Adipose tissue distribution is closely related to metabolic disease risk. GH reduces visceral and total body adipose tissue mass, while inducing whole body insulin resistance.Objective: To assess the role of visceral adipose tissue and derived adipokines for the systemic insulin resistance and lipid metabolism, before and after treatment in acromegaly.Patients: Sixty-six adult patients with active acromegaly diagnosed betw...

ea0026p192 | Pituitary | ECE2011

Adipocytes as a source of increased circulating levels of NAMPT/visfatin in active acromegaly

Olarescu C , Ueland T , Lekva T , Dahl T B , Aukrust P , Halvorsen B , Bollerslev J

Background: Nicotinamide phosphoribosyltransferase (NAMPT)/PBEF/visfatin is a widely expressed cytokine with various effects on glucose and lipid metabolism, cell survival, and inflammation. Despite a favorable body composition profile, acromegalic patients present insulin resistance, increased cardiovascular risk, and higher incidence of secondary tumors.Aim: Based on its relation to glucose and lipid metabolism and inflammation we hypothesized that NAM...

ea0073aep26 | Adrenal and Cardiovascular Endocrinology | ECE2021

Adrenal steroid profiling in the diagnostics of partial enzyme defects in the adrenals. Establishment of normal cut-off levels using LC-MS/MS

Grethe Åstrøm Ueland , Dahl Sandra R. , Methlie Paal , Husebye Eystein , Thorsby Per Medboe

BackgroundNon-classical congenial adrenal hyperplasia (NCCAH) is an important differential diagnosis in women with acne, hirsutism, menstrual abnormalities and infertility. To diagnose NCCAH can be challenging, and currently used cutoff levels are based on unstandardized immunological assays, no longer in use.ObjectiveDefine LC-MS/MS based cut-off levels for steroid hormones, to improve diagnosis of NCCAH and...

ea0029oc1.6 | Pituitary Clinical I | ICEECE2012

Normal weight adult patients with Prader–Willi syndrome are not protected from insulin resistance during treatment with GH: results from a 12 month prospective study

Jorgensen A. , Ueland T. , Sode-Carlsen R. , Schreiner T. , Rabben K. , Farholt S. , Hoybye C. , Christiansen J. , Bollerslev J.

Background: Diabetes mellitus is prevalent in adults with Prader–Willi syndrome (PWS), and GH therapy may deteriorate glucose balance.Design: We prospectively investigated effects of 12 months of GH treatment on body composition and insulin resistance in relation to BMI in forty-two adults, mean(±S.D.) age 28.5±6.7 years with genetically verified PWS. Three patients with known diabetes were excluded. Data from baseline and 1...

ea0081p144 | Pituitary and Neuroendocrinology | ECE2022

Gene expression profiling of subcutaneous adipose tissue reveals new biomarkers in active acromegaly

Maria Falch Camilla , Arlien-Soborg Mai C. , Dal Jakob , Sundaram Arvind , Michelsen Annika E. , Ueland Thor , Olsen Linn G. , Heck Ansgar , Bollerslev Jens , Jorgensen Jens Otto , Olarescu Cristina

Objective: Patients with acromegaly present increased insulin resistance despite reduced adipose tissue (AT) mass. Growth hormone (GH) stimulates lipolysis, but the role of AT as a metabolic factor in patients with acromegaly is still uncertain. Moreover, there is a need for better biomarkers of disease activity in acromegaly.Methods: RNA-sequencing was performed on paired subcutaneous AT (SAT) biopsies from patients (n=6) with active acromegaly...

ea0063gp111 | Calcium and Bone 2 | ECE2019

Hip structure analyses in acromegaly: Decrease of cortical bone thickness after treatment

Godang Kristin , Lekva Tove , Normann Kjersti R , Olarescu Nicoleta Cristina , Oystese Kristin AB , Kolnes Anders , Ueland Thor , Bollerslev Jens , Heck Ansgar

Background: Long standing growth hormone (GH) excess causes the skeletal clinical signs of acromegaly with typical changes in bone geometry including increased cortical bone thickness (CBT). However, a high prevalence and incidence of vertebral fractures has been reported. The aim of this study was to assess the course of cortical bone dimensions in the hip by comparing patients with acromegaly and clinically non-functioning pituitary adenomas (NFPA) at baseline and one year a...