Searchable abstracts of presentations at key conferences in endocrinology

ea0035p601 | Endocrine tumours and neoplasia | ECE2014

Expression of proliferating markers: PCNA and Cdk1 in hyperplastic lesions of the parathyroid glands

Segiet Oliwia , Bula Grzegorz , Deska Mariusz , Gawrychowski Jacek , Wojnicz Romuald

Background: Proliferating cell nuclear antigen (PCNA) is a cell cycle marker protein. It interacts with more than 100 proteins involved in DNA replication, DNA repair, cell cycle control, chromatin remodeling/epigenetic inheritance, chromatid cohesion and transcription. Cyclin-dependent kinase 1 (Cdk1) is a serine/threonine protein kinase which regulates diverse cell cycle transitions (G1/S, S and G2/M phases) and associates with different cell-cycle stage-specific cyclins. Bo...

ea0035p602 | Endocrine tumours and neoplasia | ECE2014

Immunohistochemical assessment of parafibromin in primary hyperparathyroidism

Segiet Oliwia , Deska Mariusz , Bula Grzegorz , Gawrychowski Jacek , Wojnicz Romuald

Background: Parafibromin is an ubiquitously expressed protein and a member of the polymerase-associated factor 1 complex associated with RNA polymerase II, which regulates transcription elongation, histone modification and cell proliferation. It is encoded by the CDC73 gene also known as the HRPT2 gene. Endogenous parafibromin inhibits expression of MYC gene that encodes the c-Myc proto-oncogene. Parafibromin has three nuclear localization signals, a...

ea0041ep977 | Thyroid (non-cancer) | ECE2016

Immunohistochemical expression of iNOS and eNOS in thyroid goiter

Segiet Oliwia , Michalski Marek , Piecuch Adam , Brzozowa-Zasada Marlena , Matysiak Natalia , Wojnicz Romuald

Introduction: Oxidative stress has been implicated in the pathogenesis of many thyroid diseases, such as non-toxic nodular goiter and Hashimoto’s thyroiditis. Nitric oxide synthases are responsible for the synthesis of nitric oxide from L-arginine. There are three isoforms of nitric oxide synthase (NOS): neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible NOS (iNOS). They have important functions in inflammation and vasoregulation but their role in thyroid diseases ...

ea0035p603 | Endocrine tumours and neoplasia | ECE2014

Apoptotic cell index in non-neoplastic lesions of the parathyroid glands

Segiet Oliwia , Michalski Marek , Piecuch Adam , Gawrychowski Jacek , zaba Malgorzata , Wojnicz Romuald

Background: Primary hyperparathyroidism is one of the most common endocrine disorders caused by adenoma (80%), hyperplasia (15%) and carcinoma (5%). It is often difficult to differentiate between hyperplasia from an adenoma of a parathyroid gland. Accordingly, the aim of this study was to assess apoptotic index as a potentially useful in diagnosis.Methods: Apoptotic cells was investigated in the parathyroid specimens of 21patients (4M, 17F, average age o...

ea0011p218 | Cytokines and growth factors | ECE2006

The role of IL-4 and IFN-gamma as prognostic factors of major adverse cardiac events in patients with ST-elevation myocardial infarction assigned primary percutaneous coronary intervention

Osuch M , Szygula-Jurkiewicz B , Szkodzinski J , Wojnicz R , Lekston A , Polonski L , Blazelonis A , Zubelewicz-Szkodzinska B

Mediators of inflammatory reaction are present at every stage of forming of atheromatous plaque, its proliferation and destabilization. Cytokines play a key role in regulation of intensity of chronic inflamatory reaction. We aimed at investigation if IL-4 and IFN-gamma are predictors of MACE in a group of patients with acute myocardial infarction (AMI).A total of 50 patients: 40 males and 10 females, aged 56±10 years (mean±S.D.),...

ea0056p655 | Endocrine tumours and neoplasia | ECE2018

Expression of Toll/interleukin-1 receptor (TIR)-associated protein in primary hyperparathyroidism.

Segiet Oliwia , Piecuch Adam , Brzozowa-Zasada Marlena , Deska Mariusz , Bula Grzegorz , Gawrychowski Jacek , Wojnicz Romuald

Background: Primary hyperparathyroidism is one of the most common endocrine disorders caused by adenoma (80%), hyperplasia (15%) and carcinoma (5%). It is often difficult to differentiate between hyperplasia from an adenoma of a parathyroid gland. Toll/interleukin-1 receptor (TIR)-associated protein (TIRAP) is an adaptor protein for Toll-like receptors-2 and -4 (TLR2/4) which are engaged in transducing the signal to downstream molecules. Several studies have shown the increase...

ea0056p656 | Endocrine tumours and neoplasia | ECE2018

Expression of TICAM-2 in hyperplastic lesions of the parathyroid glands.

Segiet Oliwia , Piecuch Adam , Brzozowa-Zasada Marlena , Deska Mariusz , Bula Grzegorz , Gawrychowski Jacek , Wojnicz Romuald

Background: Even if a great number of studies have been developed recently, the molecular mechanisms of pathogenesis of hyperplastic lesions of the parathyroid glands are not well understood. The link between systemic inflammation and promotion of neoplasm is well established. Chronic infection and inflammation are considered two of the most prominent epigenetic and environmental factors contributing to tumor formation. Toll like receptors (TLRs) are essential components of in...

ea0041ep640 | Endocrine tumours and neoplasia | ECE2016

gp91-phox in differential diagnosis of hyperplastic lesions in parathyroid gland

Segiet Oliwia , Deska Mariusz , Mielańczyk Łukasz , Brzozowa-Zasada Marlena , Buła Grzegorz , Helewski Krzysztof , Wojnicz Romuald

Introduction: Glycoprotein gp91-phox is an essential component of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox. gp91-phox is a significant element of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain th...

ea0041ep997 | Thyroid (non-cancer) | ECE2016

Markers of apoptosis in thyroid goiter

Segiet Oliwia , Mielańczyk Łukasz , Michalski Marek , Matysiak Natalia , Klymenko Olesya , Helewski Krzysztof , Wojnicz Romuald

Introduction: Thyroid goiter may be present in different thyroid pathologies, such as non-toxic nodular goiter (NTNG) or Hashimoto’s thyroiditis (HT), as a result of focal hyperplasia of thyrocytes (nodal goiter) or generalized enlargement of thyroid (diffuse goiter). Previous studies have demonstrated that programmed cell death, known as apoptosis, plays an important role in the pathogenesis of many thyroid diseases, especially inflammatory conditions.<p class="abste...

ea0041ep1033 | Thyroid (non-cancer) | ECE2016

Immunohistochemical assessment of catalase in lesions of thyroid gland

Michalski Marek , Segiet Oliwia , Piecuch Adam , Brzozowa-Zasada Marlena , Helewski Krzysztof , Harabin-Słowińska Marzena , Wojnicz Romuald

Introduction: Catalase is an enzyme, which functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It is responsible for protecting the cell from oxidative damage by reactive oxygen species. Catalase promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. Nevertheless, the role of this enzyme in thyroid pathologies i...