Endocrine Abstracts (2004) 7 P65

Leptin increases lipid peroxidation in the mouse brain

S Kutlu1, S Canpolat1, A Yasar1, M Tuzcu2, M Aydin1 & G Baydas1


1Department of Physiology, Medical School, Firat University, 23119, Elazig, Turkey; 2Department of Biology, Faculty of Science and Art, Firat University, 23119, Elazig, Turkey.


Leptin hormone is mainly secreted from adipose tissue and has an important role in energy metabolism. There have been very few studies examining effects of leptin on oxidant stress. The aim of the present study was to investigate whether leptin affects lipid peroxidation and antioxidant status in the brain of mice. Adult male Bulb-c mice were divided into two groups. Leptin was intraperitoneally administered to the first group at a dose of 40ug/animal for 5 days. Controls received phosphate buffer alone. All animals were decapitated one hour after the last injection, and brain tissues were removed. Total brain tissues were homogenized with phosphate buffered saline. Lipid hydroperoxide (which reflects the degree of lipid peroxidation level better than other peroxidation products such as malondialdehyde) and glutathione levels were analysed using specific kits by enzyme immunoassay. Data were statistically analysed by using Mann Whitney-U Test. Lipid hydroperoxide levels in the leptin-treated group (2.20±0.38 nmol/gram tissue, mean±SEM, n=6) was significantly elevated as compared with the control group (3.44±0.36 nmol/gram tissue, n=6, p<0.01). Leptin administration resulted in a significant decrease in glutathione levels (p<0.05) in total brain tissue compared to the control group values (12.97±1.32 and 17.91±0.82 nmol/gram tissue, respectively). The results of this study suggest that leptin may be of importance in generation of lipid peroxidation and inhibition of antioxidant system in the mouse brain.

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