Searchable abstracts of presentations at key conferences in endocrinology
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8th European Congress of Endocrinology incorporating the British Endocrine Societies

Oral Communications

Diabetes and metabolism

ea0011oc25 | Diabetes and metabolism | ECE2006

Cannabinoids increase AMP-activated protein kinase (AMPK) enzyme activity in the hypothalamus and heart via different signalling pathways – studies in CB1 knockout animals

Kola B , Christ-Crain M , Wittman G , Leontiou C , Grossman A , Fekete C , Korbonits M

We have recently reported that cannabinoids can stimulate hypothalamic and heart AMPK activity and can inhibit liver and adipose tissue AMPK activity in rats (Kola et al., JBC, 2005). These data are in accordance with the known orexigenic and adipogenic cannabinoid effects and also with their beneficial effects on the ischaemic heart. We have studied the effects of cannabinoids on AMPK activity in tissues from wild type (WT) and CB1 knockout (KO) mice to see if the CB1 ...

ea0011oc26 | Diabetes and metabolism | ECE2006

Neuropeptide S is a novel peptide which potently stimulates the hypothalamo-pituitary-adrenal axis and inhibits food intake

Smith KL , Patterson M , Dhillo WS , Patel SR , Semjonous NM , Gardiner JV , Ghatei MA , Bloom SR

Neuropeptide S (NPS) is a recently discovered peptide shown to be involved in the modulation of arousal and fear responses. It has also been shown that lateral ventricle administration of NPS causes a significant decrease in food intake. Neuropeptides involved in the modulation of arousal have been shown to be involved in the regulation of the hypothalamo-pituitary adrenal (HPA) axis and food intake.Objective: To examine the effect of NPS on the regulati...

ea0011oc27 | Diabetes and metabolism | ECE2006

A selective increase in adipose 11β-HSD1 expression in corticosterone-treated Pomc null mice is associated with development of metabolic syndrome

Michailidou ZM , Coll APC , Morton NMM , Kenyon CJ , O’Rahilly S , Seckl JR , Chapman KE

Inactivating mutations of the POMC gene in humans and mice result in a complex phenotype of hyperphagia, obesity and glucocorticoid (GC) deficiency, but without many features of metabolic syndrome. We have previously shown that replacement of GC in Pomc−/− mice exacerbates their hyperphagia, increases fat mass and elevates plasma leptin and insulin. We have now examined key determinants of glucocorticoid action, including glucocorticoid receptor (...

ea0011oc28 | Diabetes and metabolism | ECE2006

11β-Hydroxysteroid dehydrogenase type 1 oxo-reductase activity is increased in patients with alcoholic chronic liver disease: the key to the phenotype of the alcoholic pseudo-Cushing’s state?

Ahmed A , Saksena S , Elias E , Stewart PM

The pseudo-Cushing’s syndrome that accompanies both acute alcohol ingestion and alcohol withdrawal is an important differential diagnosis of hypercortisolism that is poorly understood. Two isozymes of 11β-hydroxysteroid dehydrogenase (11β-HSD) interconvert hormonally active cortisol (F) and inactive cortisone (E). Previously we have shown higher urinary F:E metabolite ratios (a reflection of total body 11βHSD activity) in patients with alcoholic liver disea...

ea0011oc29 | Diabetes and metabolism | ECE2006

A choline deficient diet in mice prevents high fat diet induced insulin resistance in spite of more severe fatty liver disease

Raubenheimer PJ , Nyirenda MJ , Walker BR

Fatty liver disease is strongly associated with insulin resistance. In order to elucidate the causality of this complex relationship, we studied insulin resistance in a rodent model of fatty liver disease – the choline deficient diet (CDD) – in which fatty liver occurs without generalised obesity. C57Bl/6 mice were fed a low fat (10% calories as fat, ‘Lo’) or isocaloric high fat diet (45% calories as fat, ‘Hi’) for 3 weeks; then half of the animal...

ea0011oc30 | Diabetes and metabolism | ECE2006

The release of the adipocytokine visfatin is regulated by glucose and insulin

Haider DG , Schaller G , Kapiotis S , Maier C , Luger A , Wolzt M

Background/aim: The novel insulin-mimetic adipocytokine visfatin has been linked to the metabolic syndrome but how the release of the hormone itself is regulated is not characterized yet. The aim of this study was to investigate the effect of different glucose and insulin concentrations on visfatin plasma levels in humans, and to study the involved pathways in vitro in human adipocytes.Methods: Randomized, double-blind, placebo-controlled crossove...

ea0011oc31 | Diabetes and metabolism | ECE2006

Comparative analysis of the effects of dehydroepiandrosterone (DHEA) on white and brown pre-adipocyte proliferation/differentiation

Rice SPL , Wang E , Scanlon MF , Ludgate M , Rees DA

Dehydroepiandrosterone (DHEA) is an adrenal sex steroid whose levels decline during normal aging. Epidemiological studies demonstrate inverse correlation between circulating DHEA-sulphate and body fat mass while DHEA administration in elderly subjects reduces visceral and subcutaneous fat accumulation. Although previous studies have shown that some effects may be mediated via DHEA-induced inhibition of white pre-adipocyte proliferation, mechanisms remain unknown. Furthermore, ...

ea0011oc32 | Diabetes and metabolism | ECE2006

The in vitro effects of resistin on the innate immune signalling pathway in isolated human subcutaneous adipocytes.

Kusminski CM , da Silva NF , Harte AL , Kumar S , McTernan PG

Obesity-associated inflammation is a major contributory factor in the pathogenesis of type 2 diabetes mellitus (T2DM). However, the mechanisms underlying the progression of T2DM are yet to be fully elucidated. The adipokine, resistin, has recently been implicated as a pro-inflammatory cytokine in obesity-related T2DM. Therefore, the aim of this study was to characterise the role of resistin in the innate immune inflammatory pathway within isolated human abdominal subcutaneous ...