Background: This study was carried out to assess the vasodilatory effect of oestrogen in the human pulmonary circulation. The influence of gender upon the response to oestrogen was also assessed.
Method: Isolated human pulmonary arteries were studied by wire myography. Vessels were obtained from male (n=6, age 70±9 years) and female (n=6, age 59±9 years) patients. The study was approved by the local ethics committee. Vessels were preconstricted with U46619 (1 μM) and endothelial integrity was tested with acetylcholine (1 μM). Vessels were then washed before the addition of increasing concentrations of U46619 (1nM-100μM) prior to exposing them to either oestrogen (1 nM100 μM) or ethanol vehicle.
Results: Results are shown in
Conclusion: Oestrogen acts as an efficacious vasodilator in the human pulmonary circulation, with no marked differences observed in the response dependant on sex. Oestrogen may therefore be a potential novel agent in the treatment of pulmonary vascular disease, namely pulmonary hypertension.
|Gender||0.1 μM||1.0 μM||3 μM||10 μM||30 μM||100 μM|
|*Represents significant dilation compared to ethanol vehicle (P<0.05 via students t test for independent variables).|