Searchable abstracts of presentations at key conferences in endocrinology
Endocrine Abstracts (2006) 11 P364

ECE2006 Poster Presentations Diabetes, metabolism and cardiovascular (174 abstracts)

Anti-oxidative effect of 17beta-estradiol in human endothelial cells: the role of Bcl-2

W Zhong & SL Atkin

59 views


University of Hull, Hull, North Yorkshire, United Kingdom.


Aim: To determine if the endogenous estrogen 17beta-estradiol protected against oxidative stress-induced endothelial cell damage and the mechanism of this potential effect.

Methods: Human umbilical vein endothelial cells were isolated and cultured in phenol red free media with 2% charcoal-stripped serum. The following were undertaken (1) Cells were exposed to 0.1 to 1 nM 17beta-estradiol immediately prior to 100 μM hydrogen peroxide added for 24 hours, with and without the anti-estrogen ICI182,780. Cell proliferation, apoptotic DNA fragmentation and expression of Bcl-2 and Bax were subsequently measured by tritiated thymidine incorporation assay, Cell Death ELISA and Taqman quantitative PCR, respectively. (2) Cell Bcl-2 expression in response to 17beta-estradiol treatment was determined by Taqman quantitative PCR and western blotting. (3) Cells with or without Bcl-2 siRNA transfection (Bcl-2 knockdown) were exposed to 17beta-estradiol and hydrogen peroxide, and cell DNA fragmentation was then detected. The outcome of the Bcl-2 gene knockdown was confirmed by Taqman quantitative PCR and western blotting.

Results: Hydrogen peroxide significantly reduced cell proliferation, increased cell apoptotic DNA fragmentation level and caused reduced mRNA expression of Bcl-2 but elevated Bax. All of these were significantly protected against by 17beta-estradiol but reversed by the anti-estrogen ICI182780. 17beta-estradiol dramatically increased Bcl-2 expression at both mRNA and protein levels. Bcl-2 expression was silenced by the siRNA transfection, and within these cells, the protective effect of 17beta-estradiol on hydrogen peroxide induced apoptosis was completely lost.

Conclusion: 17beta-estradiol had protective effect against oxidative stress induced cell proliferation inhibition and cell apoptosis in human endothelial cells in vitro, mediated through a Bcl-2 dependent pathway.

Volume 11

8th European Congress of Endocrinology incorporating the British Endocrine Societies

European Society of Endocrinology 
British Endocrine Societies 

Browse other volumes

Article tools

My recent searches

No recent searches.