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Endocrine Abstracts (2006) 11 P728

ECE2006 Poster Presentations Steroids (44 abstracts)

Capillary blood, an alternative to saliva for the diagnosis of Cushing’s syndrome?

N Belaidi 1 , S Mimouni 1 , C Ourioux 1 , B Gatta 2 & JB Corcuff 1


1Médecine nucléaire, Hôpital Haut-Lévêque, Pessac, France; 2Endocrinologie, Hôpital Haut-Lévêque, Pessac, France.

Introduction: As an alternative to cortisol assays in the serum or saliva to diagnose hypercorticisme we evaluated the ability of a commercial cortisol assay to assess capillary blood cortisol concentrations using routine procedures.

Methods: Capillary blood was obtained using a device used by diabetics to routinely assess capillary glucose levels and deposited on Schleicher-Scheull 903 blotter paper (routinely used for neonatal diagnosis of hypothyroidism, CAD…). In separate experiments, blood was sampled from veins into EDTA-containing tubes to allow precise volume distribution on the blotter paper (15 μL). Discs were punched from blotter paper (6.5 mm Ø). Two solid phase cortisol RIA (Gamma-Coat Diasorin) were performed: method A routine assay in serum, or method B modified procedure to assay in saliva.

Results: There was a significant relationship between serum cortisol and capillary blood cortisol whatever the method used: r2 0.83 & 0.87 (A & B methods respectively). A multivariate analysis did not show any influence of hematocrite.

Intra- and inter-assay coefficients of variation were: 13 & 5 and 13 & 11%, (A & B methods respectively).

Detection threshold (intra-assay CV <20%) were 40 & 2.7 nmol/l corresponding to 40 & 80 nmol/l serum cortisol levels (A & B methods respectively).

Conclusion: Capillary blood cortisol assessment is reliable enough to initially investigate diurnal rhythm or minute dexamethasone test in patients in which venous sampling in notoriously difficult. Advantages of method A are the low detection threshold and the procedure identical to serum assay allowing simultaneous management of serum and capillary samples; advantages of method B is to allow duplicate assay.

Volume 11

8th European Congress of Endocrinology incorporating the British Endocrine Societies

European Society of Endocrinology 
British Endocrine Societies 

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