Searchable abstracts of presentations at key conferences in endocrinology
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10th European Congress of Endocrinology

Oral Communications

Bone and adrenal

ea0016oc4.1 | Bone and adrenal | ECE2008

Inactivation of the Carney complex (CNC) gene 1 (PKA regulatory subunit 1A, PRKAR1A) by interference RNA alters multiple signaling pathways and decreases apoptosis

Ragazzon Bruno , Cazabat Laure , Rizk-Rabin Marthe , Perlemoine Karine , Martinez Antoine , Bertherat Jerome

The cAMP signaling pathway plays an important role in cell proliferation and differentiation, and can be altered at multiple levels in endocrine tumors. Its central component is the protein kinase A (PKA). Inactivating mutations of PRKAR1A are observed in CNC (a dominant autosomal hereditary disease responsible for primary pigmented nodular adrenocortical disease, cardiac myxoma and lentiginosis). Most PRKAR1A mutations lead to mRNA unstability and protein degrad...

ea0016oc4.2 | Bone and adrenal | ECE2008

Reduced fertility rates and high prevalence of testicular adrenal rest tumors (TART) in male patients with congenital adrenal hyperplasia

Reisch Nicole , Flade Linda , Reincke Martin , Beuschlein Felix

Objective: To evaluate reduced fertility rates and their possible causes in a cohort of well controlled male adult patients with congenital adrenal hyperplasia.Methods: We clinically assessed 22 male patients with congenital adrenal hyperplasia (15 salt wasting form, 7 simple virilising, age 18–49) according to their hormonal control. Further, we performed testicular ultrasound, MRI of the testis and a sperm count of each. As laboratory markers 17-O...

ea0016oc4.3 | Bone and adrenal | ECE2008

BMP dependent modulation of adrenocortical growth and function

Johnsen Inga K , Spady Lilia , Shapiro Igor , Beuschlein Felix

Bone morphogenic proteins (BMPs) have been demonstrated to impact tumorigenesis in a variety of tumors. As for the adrenal cortex, BMP6 has been implicated as an important modulator of aldosterone secretion. To screen for alterations of BMP dependent pathways in adrenal tumorigenesis we performed gene profiling experiments. By comparing human adrenal carcinoma (ACC) against normal adrenal glands (Co) we detected a down-regulation of various BMPs (e.g. BMP2, BMP5) which was fur...

ea0016oc4.4 | Bone and adrenal | ECE2008

Comparison of different test systems for the screening of inhibitors of CYP11B enzymes

Stuermer Andrea , Hahner Stafanie , Lang Katharina , Linss Heidi , Zink Martina , Schirbel Andreas , Allolio Bruno

Subject: Inhibitors of CYP11B enzymes, particularly of aldosterone synthase, are currently under development as adrenostatic agents in heart failure or hyperaldosteronism, and for use in adrenal imaging. As CYP11B1 and CYP11B2 show high homology, analysis of specific binding to the respective human enzymes is of key importance.Methods: Y1 cell lines stably expressing hsCYP11B1 or hsCYP11B2 were established. Primary cultures of porcine and human adrenocor...

ea0016oc4.5 | Bone and adrenal | ECE2008

Comparison of 1-mg versus 8-mg dexamethasone suppression tests (DST) in patients with clinically inapparent adrenal adenoma

Reimondo Giuseppe , Bovio Silvia , Daffara Fulvia , Allasino Barbara , Micossi Ilaria , Zaggia Barbara , Ardito Arianna , Angeli Alberto , Terzolo Massimo

Recent guidelines recommend to use the 1-mg DST to screen for subclinical hypercortisolism assuming that a post-DST cortisol >5.0 μg/dl define this condition. However, several experts suggest a more sensitive cut-off at 1.8 μg/dl while others consider mandatory to confirm unsuppressibility with a 8-mg DST. A consecutive series of 64 patients (22 men, 42 women, aged 28–81 years) with clinically inapparent adrenal adenoma were studied between 2005 and 2007. Th...

ea0016oc4.6 | Bone and adrenal | ECE2008

Isolation and characterization of cells coexpressing endothelial progenitors and parathyroid specific genes from human adult normal and tumoral parathyroids

Corbetta Sabrina , Belicchi Marzia , Pisati Federica , Meregalli Mirella , Eller-Vainicher Cristina , Vicentini Leonardo , Beck-Peccoz Paolo , Spada Anna

A peculiar characteristic of parathyroid tissue is the ability to spontaneously induce angiogenesis, to proliferate and to secrete PTH when autotransplanted in patients undergoing total parathyroidectomy. Since stem/progenitor cells have been involved in the process of tissue regeneration, we searched for putative parathyroid progenitors from human normal and tumoral parathyroids. By immunohistochemistry, FACS analysis and cell culture we identified parathyroid cells positive ...

ea0016oc4.7 | Bone and adrenal | ECE2008

Molecular analysis of the calcium sensing receptor (CaSR) gene in 40 patients suspected to have familial hypocalciuric hypercalcemia (FHH)

Defrance-Faivre Frederique , Odou Marie-Francoise , Porchet Nicole , Weill Jacques , Guedj Am , Cardot-Bauters Catherine , Wemeau Jean-Louis , Vantyghem Marie-Christine

Neonatal severe hyperparathyroidism (NSHPTH) and FHH, usually defined as a ratio of calcium clearance/creatinin clearance <0.01 with normal kidney function and vitamin D status, are caused by respectively heterozygote and homozygote inactivating mutations of the CaSR gene. The aim of this study was to assess the interest of analyzing CaSR in hypercalcalcemic subjects suspected to have FHH.Patients and methods: Forty hypercalcaemic subjects fro...

ea0016oc4.8 | Bone and adrenal | ECE2008

Incidental parathyroidectomy during thyroid surgery: outcome, follow-up and management of 231 patients treated between 1989 and 2007

Feldmann Berit , Bacher Konrad , Cordes Astrid , Grussendorf Martin

Background: Hypocalcemia is one of the most frequent complications after thyroid surgery. The aim of this study was to investigate the clinical relevance, outcome, follow-up and management of patients with incidental parathyroidectomy during thyroid surgery.Subjects and methods: Two hundred and thirty-one patients (5.2%, 200 females and 31 males) of 4206 operated patients, which presented between 1989 and 2007 in our out-patient endocrine department, suf...

ea0016oc4.9 | Bone and adrenal | ECE2008

Teriparatide (TPTD) treatment followed by either zoledronic acid (ZOL) 5 mg once yearly or strontium ranelate (SR) 2 g daily: preliminary results from the ZOSTER-Study

Riedmuller Gunter , Fahrleitner Astrid , Dobnig Harald , Piswanger Claudia , Stiegler Claudia , Stepan Vinzenz , Obermayer-Pietsch Barbara , Hans-Peter Dimai

Background: TPTD treatment has been shown to reduce vertebral and non-vertebral fracture risk in postmenopausal osteoporosis efficiently. However, gains in bone mass achieved by this treatment are almost lost 2 years after cessation of treatment, unless consecutively followed by an antiresorptive treatment such as alendronate.Subjects and methods: In the present prospective randomised open-labelled study in 52 pmp women, we investigated the effect of eit...