Searchable abstracts of presentations at key conferences in endocrinology
Previous issue | Volume 16 | ECE2008 | Next issue

10th European Congress of Endocrinology


Basic highlights

ea0016s14.1 | Basic highlights | ECE2008

Direct visualization of cyclic AMP levels in three dimensional cultures of thyroid follicles

Calebiro Davide , Nikolaev Viacheslav , Bonomi Marco , Persani Luca , Lohse Martin

Cyclic AMP (cAMP) is the principal intracellular mediator of TSH effects in the thyroid, inducing both thyroid hormone production and cell proliferation. This notion derives from a large series of evidences, mainly obtained by biochemical approaches on cell lines or primary thyrocytes. However, given the limited resolution in time and space of the techniques employed so far, little is know about the spatial localization and temporal dynamics of cAMP signaling in thyroid cells....

ea0016s14.2 | Basic highlights | ECE2008

Dopamine receptor 2 activation reduces cells viability in non-functioning pituitary adenomas by inhibiting vascular endothelial growth factor secretion

Zatelli Maria Chiara , Tagliati Federico , Luchin Andrea , Ambrosio Maria Rosaria , Cimino Vincenzo , Bondanelli Marta , Scanarini Massimo , Maira Giulio , De Marinis Laura , degli Uberti Ettore

Dopamine (DA) therapy of non-functioning pituitary adenomas (NFA) can result in tumor stabilization and shrinkage. However, this effect is not always apparent and the mechanism of action is still unknown. Previous evidence showed that DA inhibits pituitary Vascular Endothelial Growth Factor expression (VEGF), that, in turn, is related to pituitary tumor growth. Our study aimed at clarifying whether VEGF secretion modulation might mediate the effects of DA agonists and chimeric...

ea0016s14.3 | Basic highlights | ECE2008

Variants of the phosphodiesterase 11A (PDE11A4) gene and genetic predisposition to adrenocortical tumors (ACT)

Libe Rossella , Fratticci Amato , Coste Joel , Groussin Lionel , Horvath Anelia , Rene-Corail Fernande , Bertagna Xavier , Raffin Sanson Marie-Laure , Stratakis Constantine , Bertherat Jerome

Introduction: We have previously identified (using a whole-genome large scale SNP- screening approach) germline inactivating stop codon mutations of the PDE11A4 in patients with Cushing syndrome due to micronodular adrenal hyperplasia. PDE11A4 is a cAMP/cGMP phosphodiesterase.Aim of the study: To investigate the role of PDE11A genetic alterations in a large cohort of ACT.Materials and methods: Leukocyte DNA from 117 adrenocortical ...

ea0016s14.4 | Basic highlights | ECE2008

Targeted disruption of Slc2a8 (GLUT8) reduces ATP levels and mitochondrial potential of spermatozoa

Gawlik Verena , Schmidt Stefan , Scheepers Andrea , Wennemuth Gunther , Augustin Robert , Moser Markus , Al-Hasani Hadi , Joost Hans-Georg , Schurmann Annette

GLUT8 is a class 3 sugar transport facilitator, and transports glucose with high affinity (km~2 mM). GLUT8 mRNA is expressed in brain, heart, skeletal muscle, adipose tissue, adrenal gland, liver and at particulary high levels in testis. In testis, the GLUT8 protein is located in an intracellular compartment of spermatocytes, spermatids and mature spermatozoa. GLUT8 contains an N-terminal dileucine sorting signal retaining the transporter in an intracellular compartment. So fa...

ea0016s14.5 | Basic highlights | ECE2008

KLF11 differentially regulates PDX1-induced activation of the human insulin promoter in rodent beta-cells and human non-beta-cells

Perakakis Nikolaos , Path Gunter , Niu Xinjie , van Loosen Sandra , Alt Marcus , Stahl Timm , Seufert Jochen

KLF11 is a pancreas enriched member of the Sp1/KLF transcription factor family which plays an important role in mammalian gene regulation. Previously we demonstrated that human (h)KLF11) inhibits human insulin promoter (hInsP) activity in rodent beta-cell lines via an unknown mechanism. Here we present results from ongoing experiments in which we test the hypothesis that KLF11 interferes with the major hInsP transactivator PDX1. We performed transient cotransfections of INS-1E...

ea0016s14.6 | Basic highlights | ECE2008

Specific neuronal deletion of cannabinoid type 1 receptor (CB1) mice exhibits reduced fat mass and diet-induced obesity resistance

Cervino Cristina , Bellocchio Luigi , Quarta Carmelo , Mariani Corea Vania A , Mancini Giacomo , Vicennati Valentina , Lutz Beat , Marsicano Giovanni , Pasquali Renato , Pagotto Uberto

Aims: Recently, the endocannabinoid system emerged as a pivotal regulator of food intake, ingestive behavior and energy metabolism, acting through CB1 and its endogenous ligands, the endocannabinoids. CB1 antagonists may reduce body weight and improve metabolic profiles in animals and humans by a double mechanism: at first, they target mesolimbic and hypothalamic nuclei and, thereafter, peripheral organs involved in energy storage and expenditure. Nevertheless, it is still unk...