The aim of this study was to simultaneously profile, using electrospray ionization liquid chromatography mass spectrometry (ESI-LC-MS), prostanoids (PG) produced in samples of: pregnant preterm non labouring (PTNL); term non labouring (TNL): and term labouring (TL) myometrium.
Lower segment samples were obtained at Caesarean section from consenting pregnant women (1836 years of age) at term (3841 weeks) and preterm (33 weeks) gestation. Samples were transported to the laboratory and immediately bathed in physiological Krebs solution±indometacin (1 μM) for 1 h at 4 °C (samples without indometacin are referred to as untreated (U) or treated (T) if indometacin was present), prior to freezing and subsequent solid phase extraction. Extracts analysed using ESI-LC-MS were quantified using calibration lines made up of commercially available standards. Results are expressed as mean pg/mg protein (as estimated by Lowry method).
|PTNL (n=1)||TNL (n=6)||TL (n=3)|
|In U-PTNL myometrium, the three most abundant PGs (ranked from highest to lowest) were: TXB2, 6-keto-PGF1α and PGD2||In TNL(U+T) myometrium, the three most abundant PGs (ranked from highest to lowest) were: 6-keto-PGF1α, PGD2 and PGF2α||In TL (U+T) myometrium, the three most abundant PGs (ranked from highest to lowest) were: 6-keto-PGF1α, PGF2α and PGD2|
Disruption of the thromboxane/prostacyclin balance may be implicated in the onset of preterm labour. Our data are consistent with the role of prostacyclin as a mediator of uterine quiescence at term and the role of PGF2α as an elicitor of myometrial contractions at term labour.
03 - 07 May 2008
European Society of Endocrinology