ISSN 1470-3947 (print) | ISSN 1479-6848 (online)

Endocrine Abstracts (2008) 16 P649

Accumulation of dietary glycotoxins in the reproductive system of normal female rats

Evanthia Diamanti-Kandarakis1, Christina Piperi2, Eleni Kandaraki2, Penelope Korkolopoulou3, Efstratios Patsouris3, Apostolos Papalois4 & Athanasios Papavassiliou2


1Endocrine Section, First Department of Internal Medicine, Medical School, University of Athens, Athens, Greece; 2Department of Biological Chemistry, Medical School, University of Athens, Athens, Greece; 3Department of Pathology, Medical School, University of Athens, Athens, Greece; 4Experimental Research Unit, ELPEN Pharma, Athens, Greece.


The present study aimed to investigate whether dietary advanced glycation end-products (AGEs) are detected in the ovarian tissue of normal female rats and whether they affect metabolic or hormonal profile.

Normal rats were randomly assigned to regular diet, high (H-AGE) or low (L-AGE) content for 6 months. Age-matched rats fed with regular diet served as controls.

H-AGE rats, demonstrated higher levels of fasting glucose (P<0.001), insulin (P=0.069), and serum AGEs (P<0.001) and testosterone (P<0.001), than control and L-AGE rats. In H-AGE rats body weight compared with normal (P=0.118) and L-AGE-fed rats (P=0.35) did not differ. H-AGE group showed increased AGE localization in the theca interna cells of the ovarian tissue compared to control rats (P=0.003). Furthermore, increased receptor for AGE (RAGE) staining was also observed in both granulosa as well as in theca interna cells compared to controls (P=0.038 and P=0.052 respectively).

These results demonstrate for the first time that administration of high AGE diet in normal female rats is associated with increased deposition of AGEs in the theca cells and of RAGE in the granulosa and theca interna cells of the ovarian tissue compared with the corresponding ovarian compartments of the control and low AGE-fed animals. The metabolic alterations in conjunction with the increased deposition in ovarian tissues of dietary glycotoxins and elevated levels of testosterone in H-AGE-fed animals compared to the controls, suggest an additional impact of environmental factors on ovarian function and these findings need further exploration.

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