Endocrine Abstracts (2009) 20 P64

Association of G1201/T exon8 and A2257/C exon 12 polymorphisms with anti-TPO titter

Mehdi Hedayati, Maryam Sadat Daneshpour, Marziyeh Salehi & Fereidoun Azizi


Research Institute for Endocrine Sciences, Tehran, Islamic Republic of Iran.


Objective: The aim of this study was the assessment of association of two single nucleotide polymorphism (SNP) of thyroid peroxidase (TPO) gene with anti TPO antibody titer for the first time.

Materials and methods: In this case–control study, peripheral blood samples of 428 individuals, 178 men (29–76 y) and 250 women (21–76 y) from unrelated families were genotyped (G1201T of exon 8 and A2257C of exon 12) by PCR amplification and RLFP method. The sera anti TPO antibody titter were determined in all subjects.

Results: One hundred and seventy-eight (125 women and 53 men) individuals had positive TPO test (41.6%) and 250 (125 women and 125 men) negative (58.4%). G1201/T polymorphism was observed in 116 individuals (65.1%) with positive TPO test and 160 controls (64%). A2257/C polymorphism was observed in 110 individuals with positive TPO test (61.8%) and 30 controls (12%). There was no association between G1201/T exon8 of TPO gene and TPO auto antibodies but an association between A2257/C exon 12 and the auto antibodies was found (P<0.05).

Conclusion: For the first time our result suggests the association between A2257/C exon 12 of TPO gene and antiTPO antibodies rising.

Keywords: TPO, SNPs, polymorphism.

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