Introduction: Urtica diocia extract has been used for treatment of diabetes mellitus for many centuries. Hypoglycemic effects of U. diocia have been shown in multiple studies. The present study was designed to determinutese the possible mechanisms of hypoglycemic effects of U. diocia on RIN5F Rat Pancreatic β cells.
Methods and materials: In cell culture laboratory of Drug Applied Research Center of Tabriz University (Medical Sciences) pancreatic β cell prepared in multiple flasks containing culture media. Alcoholic extract of U. diocia with doses of 50, 100 and 200 μg were added to flasks containing RIN5F Rat Pancreatic βcell. Insulin and C-peptide levels were measured in 0, 60, 120 and 180 min.
Results: Insulin level in pancreatic cell medias before and after adding of U. diocia with varying doses and in different times were ≤0.2 μg/ml. C-peptide (μg/ml) level in these medias with dose of 50 μg of U. diocia and in above mentioned times were 0.31, 0.33, 0.86 and 0.8; with dose of 100 μg were 0.7, 0.2, 0.4 and 0.39; and with dose of 200 μg were 0.32, 0.33, 0.93, 0.77 respectively.
Conclusion: The results of the present study show that alcoholic extract of U. diocia was not able to increase Insulin and C-peptide secretion from RIN5F pancreatic βcells
25 - 29 Apr 2009
European Society of Endocrinology