Endocrine Abstracts (2009) 20 P537

The effect of gestational hypothyroidism on insulin secretion from isolated islets of adult offspring in male rats

Saleh Zahedi-Asl1, Hamid Farahani2, Asghar Ghasemi1, Farzaneh Faraji1 & Homa Manaheji2

1Endocrine Physiology Laboratory, Research Institute for Endocrine Sciences, Endocrine Research Center, Shahid Beheshti University (MC), Tehran, Islamic Republic of Iran; 2Department of Physiology, The Medical School, Shahid Beheshti University (MC), Tehran, Islamic Republic of Iran

Introduction: Any abnormalities during pregnancy can affect the growth and development of the fetus and may have a long lasting effect presented during adulthood (intrauterine programming). In this study, the effect of maternal hypothyroidism on insulin secretion from the isolated islets of offspring has been investigated in male rats.

Materials and methods: In Test group maternal hypothyroidism was induced using 0.02% propylthiouracyl in drinking water throughout the pregnancy period while the control animals consumed drinking water alone. Islets were isolated from offspring by collagenase digestion method. Islets were incubated in Krebs ringer solution containing different glucose concentration (5.6, 8.7 and 16.7 mM) in static condition for 60 min. Insulin was measured with rat specific ELISA Kit.

Results: The results indicate that the insulin secretion from isolated islets of the offspring from hypothyroid mothers were significantly reduced when were stimulated with different glucose concentration (P<0.05). Thyroid hormone measurements show that the offspring were not hypothyroid at the time of the insulin secretion assessment.

Conclusion: From the results of this study, it appears that maternal hypothyroidism can affect insulin secretion capacity and therefore its impact on carbohydrate metabolism and possible role on diabetes induction should be considered.

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