Objective: It is known that mothers stress during prenatal period of gestation provokes the firm long-lasting disturbances of fetal functional systems. The consequences of stress within early pregnance (SEP) are still researched poorly.
Aim: The aim of study was to evaluate hormonal and behaviour features in females offspring of rats with SEP.
Material and methods: For SEP creation the Wistar rat females were transferred daily into another rats association within 2nd8th days of gestation. At 12 months old the intact offspring (n=32) randomly divided into groups which were killed before (G-1) and after (G-11) functional tests. Correspondingly G-2 and G-21 were formed from stressed offspring. Corticosterone, leptin, E2, testosterone, progesterone were determined by the RIA method. Food consumption was determined by placing 200 g of fodder and weighing the residual food before and after 24 h of fasting. Perirenal, gonadal and mesenteric fat pads were removed and weighed. Data were performed statistically.
Results: It has been established that 80% animals from G-2 were heavier by 1525% than G-1. G-2 corticosterone was not distinguished from G-1 but after 24 h of fasting corticosterone was expressive increased (P<0.05, G-21 vs G-2, G-1, G-11). Offspring from G-2 had reduced leptin level after fasting and normal level through 24 h after termination of food deprivation. G-21 food consumption was increased by 1517% than in G-11 within 124 h after fasting. Weight of mesenteric fat G-21 increased by 5% through 24 h after termination of food deprivation. The stressed offspring demonstrated a high anxiety level in elevated plus-maze and decrease locomotor activity in the open field. Their testosterone level was increased by 712%, but E2 and progesterone were decreased.
Conclusion: Our findings evidence that fetus stress in the first early gestation induced negative alterations of hormonal status and behaviour in of offspring and leads to the metabolic diseases in adulthood.
Prague, Czech Republic
24 - 28 Apr 2010
European Society of Endocrinology