Introduction: Autoimmune thyroid disease (AITD) is a common disease. It arise due to interactions between genetic background and environmental factors. However this interactions remain unclear. As it induces other autoimmune diseases, parvovirus is an attractive candidate.
Objective: To investigate the presence of parvoviral DNA in postoperative thyroid tissues.
Materials and methods: We collect prospectively postoperative thyroid specimens and serum samples from 90 patients. We investigate presence of IgG antibodies on serum samples (IgG Parvovirus B19 Biotrin). Presence of parvoviral genome sequences in thyroid was investigated by real-time PCR (Artus* ParvoB19 TM PCR Kit Qiagen) in serum samples and in thyroid specimens of patients, who were IgG positive (which prove a contact with parvovirus).
Results: Thirty-three patients were IgG positive. Parvoviral DNA detection was positive in 6 out of the 33 thyroid specimens. Only 1 serum sample was DNA positive out of these 6 patients. Final diagnosis was Graves disease in 3 out of 6 DNA parvoviral positivepatients (50%) and in 3 out of 27 DNA parvoviral negative patients (11.1%) (P=0.08). Lymphocytic infiltration was observed in 5 out of the 6 DNA parvoviral positive patients (83.3%) and in 9 out of the 27 DNA parvoviral negativepatients (33.3%) (P=0.08). Two serum samples were DNA positive out of these 27 patients.
Conclusion: DNA parvoviral presence in thyroid tissue is observed and is not due to viremia. This study suggests a possible relation between Graves disease, thyroid lymphocytic infiltration and parvovirus infection. Further study are needed to confirm these results.
Prague, Czech Republic
24 - 28 Apr 2010
European Society of Endocrinology