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Endocrine Abstracts

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Society for Endocrinology BES 2011

Poster Presentations

Reproduction

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Steroids

ea0025p263 | Reproduction | SFEBES2011

Endocrine disruptors and their association with male reproductive disorders and testicular dysgenesis syndrome: establishing a xenografting model of human fetal testis development

Mitchell Rod , Saunders Philippa , Childs Andrew , Cassidy-Kojima Claire , Anderson Richard , Wallace Hamish , Kelnar Chris , Sharpe Richard

Testicular dysgenesis syndrome (TDS) is a group of associated conditions (testicular germ cell tumours (TGCT), cryptorchidism, hypospadias and low sperm counts) that are thought to have a common origin in fetal life. Exposure of fetal rats to environmental chemicals such as the endocrine disrupting chemical di(n-butyl) phthalate (DBP) results in a TDS-like syndrome. However, exposed rats do not develop TGCT and the rodent is a poor model in this context. The effects of ...
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ea0025p264 | Reproduction | SFEBES2011

Distinct expression pattern of Dicer1 correlates with ovarian-derived steroid hormone receptors in human Fallopian tubes during the ovulatory process and in the mid-secretory phase.

Shao Ruijin , Billig Hakan

Context: Tissue-specific dicer1 knockout female mice display severe and irreversible damage to the Fallopian tubes that eventually leads to disruption of tubal transport of the gametes and the early embryo. The impact of Dicer1 on the function of human Fallopian tubes remains to be clarified.Objective: To determine how the expression of tubal Dicer1 is regulated in women during the ovulatory process and in the mid-secretory phase. Furthermore, the...
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ea0025p265 | Reproduction | SFEBES2011

The effect of atorvastatin on adrenal and ovarian hyperandrogenemia in patients with polycystic ovary syndrome.

Sathyapalan Thozhukat , Smith Karen A , Coady Anne-Marie , Kilpatrick Eric S , Atkin Stephen L

Context: Hyperandrogenemia in polycystic ovary syndrome (PCOS) represents a composite of raised serum concentrations of testosterone, androstenedione, dehydroepiandrosterone (DHEA), and DHEA sulfate (DHEAS). In patients with PCOS, testosterone and androstenedione are primarily derived from the ovaries and DHEAS is a metabolite predominantly from the adrenals. It has been shown that atorvastatin reduces testosterone levels in patients with PCOS.Objective:...
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ea0025p266 | Reproduction | SFEBES2011

Abstract withdrawn....
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ea0025p267 | Reproduction | SFEBES2011

Placental iodothyronine deiodinase activity across the placental bed in normotensive (NT) and pre-eclamptic (PE) pregnancies.

Kurlak Lesia , Mistry Hiten , Kaptein Ellen , Visser Theo , Pipkin Fiona Broughton

Background: PE affects ~2% of pregnancies and is associated with generalised endothelial cell dysfunction. Antioxidant selenoproteins may limit such effects. Serum selenium is lower in mothers and babies affected by PE and associated with lower activity of the selenoprotein glutathione peroxidases. Other selenoproteins, the iodothyronine deiodinases (IDs; D2 and D3) regulate fetal thyroid hormone synthesis. The IDs utilise selenium preferentially; we have reported similar trii...
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ea0025p268 | Reproduction | SFEBES2011

In support of the ‘Rotterdam’ PCOS criteria: Identical LH responses to GnRH stimulation in women with oligo-/amenorrhoea and polycystic ovaries regardless of androgen status

Lewandowski Krzysztof , Cajdler-Luba Agata , Bienkiewicz Malgorzata , Lewinski Andrzej

Objective: There is no universal consensus as to the criteria of PCOS (i.e. Rotterdam ESHRE/ASRM versus Androgen Excess Society (AES) criteria). Furthermore, androgen measurements within the female range are fraught with several methodological problems. As GnRH stimulation can reveal a relative LH excess, caused by an increased frequency of hypothalamic GnRH pulses, then we have endeavoured to assess gonadotrophin response to GnRH in women with PCOS and healthy controls...
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ea0025p269 | Reproduction | SFEBES2011

Kisspeptin-54 injection stimulates activity of the human GnRH pulse generator in healthy women

Jayasena Channa N , Comninos Alexander N , Misra Shivani , Ali Abbara , Aniket Tavare , Donaldson Mandy , Ghatei Mohammad A , Bloom Stephen R , Dhillo Waljit S

Background: Kisspeptin is a novel hypothalamic hormone with powerful stimulatory effects on the hypothalamo-pituitary–gonadal (HPG) axis. Inactivating mutations in the kisspeptin receptor lead to pubertal failure. We have previously demonstrated that injection of kisspeptin-54 stimulates LH release in healthy men and women. Recent studies in animals suggest that endogenous kisspeptin may be involved in stimulating the GnRH pulse generator. Determining whether exogenous ad...
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ea0025p270 | Reproduction | SFEBES2011

Expression and functional activity of thyroid hormone transporters in microvillous plasma membranes from human term placental syncytiotrophoblasts

Loubiere Laurence , Vasilopoulou Elisavet , Franklyn Jayne , Kilby Mark , Chan Shiao

Background: Thyroid hormones (TH) are vital for fetal and placental development. TH transporters including monocarboxylate transporters 8 and 10 (MCT8, MCT10), organic anion transporters (OATP1A2, OATP4A1) and system-L amino acid transporters (LAT1, LAT2) are expressed in human placenta from 6 weeks of gestation. All of these TH transporters have been localized to the human syncytiotrophoblast layer of placental villi, which is in direct contact with maternal bl...
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ea0025p271 | Reproduction | SFEBES2011

Increased epididymal Rnase9 expression in Rnase10 (−/−) mice

Sharp Victoria , Krutskikh Anton , Dacheux Jean-Louis , Poutanen Matti , Huhtaniemi Ilpo

Spermatozoa are dependent on the proximal epididymis environment to complete their maturation. However, no single specific factor crucial for this process has been identified. Rnase9 and Rnase10 are expressed specifically in the murine proximal epididymis. Located on chromosome 14C1 only 28 kb apart, they do not show high homology with each other. An orthologue of both genes exist in the rat and human, and ‘Train A’ (porcine orthologue) is the most abun...
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ea0025p272 | Reproduction | SFEBES2011

Is age of vaginal opening an indicator of leptin sensitivity in female Sprague Dawley rats?

Sleeth Michelle , Beale Kylie , Thompson Emily , Baxter Jordan , Bloom Stephen , Murphy Kevin

Leptin is an adipocyte-derived cytokine critical to the integration of energy homeostasis and reproduction which putatively acts as a metabolic gate for the onset of puberty. However, it is unknown whether the age of puberty reflects leptin levels or leptin sensitivity. If pubertal age reflects leptin sensitivity, it may also reflect susceptibility to diet-induced obesity.We hypothesised that age of pubertal onset reflects leptin sensitivity. We assessed...
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ea0025p273 | Reproduction | SFEBES2011

A case of severe refractory hypercalcaemia in pregnancy caused by hypersecretion of parathyroid hormone related peptide (PTHrP) by the placenta.

Martin Allison , Spring Mark

A 39-year-old woman presented at 23 weeks gestation with extreme fatigue and non-specific neurological symptoms. Other than mild hypertension her physical examination was normal. Serum calcium was 3.36 mmol/l (normal 2.12–2.62) and phosphate 0.8 mmol/l (Normal 0.8–1.5). She was severely hypercalcemic throughout pregnancy and her corrected calcium ranged between 2.82 and 3.48 mmol/l. Other investigations included 25 hydroxy-Vitamin D3 98 (normal 75–200...
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ea0025p274 | Reproduction | SFEBES2011

Comparison of cardiovascular markers and variability of insulin resistance in women with anovulatory and ovulatory polycystic ovary syndrome

Cho Li Wei , Kilpatrick Eric , Keavil Brian , Jayagopal Vijay , Coady Anne Marie , Atkin Stephen

Objective: We aim to study whether a difference exists in cardiovascular risk and variability of IR within the hyperandrogenic group of women with PCOS who are ovulatory and anovulatory.Method: Fifty-three women with PCOS were recruited. All subjects were diagnosed to have PCOS by the ESHRE/ASRM Rotterdam criteria where all patients had evidence of biochemical hyperandrogenism and hirsutism, chronic anovulation and polycystic ovaries on transvaginal ultr...
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ea0025p275 | Reproduction | SFEBES2011

Male germ cell activity during perinatal reproductive development in the mouse

Jarvis Sheba , Winston Robert , Fraser Scott , Readhead Carol

Dynamic changes in gene expression patterns and cell behaviour are evident throughout embryonic and neonatal germ cell development in the mouse. In the testes, the postnatal period represents a time when the male germline stem cells (GSCs) or, gonocytes migrate to the basement membrane of the seminiferous tubules preparing for a lifetime of spermatogenesis and is an important area of study. Here we use the transgenic mouse that expresses green fluorescent protein under the Oct...
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ea0025p276 | Reproduction | SFEBES2011

Defining insulin signalling pathways in granulosa-lutein (GL) cells in women with polycystic ovary syndrome (PCOS)

Joharatnam Jalini , Lamanna Giuseppina , Trew Geoffrey , Lavery Stuart , Hardy Kate , Franks Stephen

Aim: PCOS is associated with peripheral insulin resistance and we have previously shown that glucose uptake and metabolism are impaired in granulosa lutein (GL) cells of anovulatory women with PCOS (anovPCO). The aim of this study was to delineate insulin-signalling pathways in GL cells from women with anovPCO and to compare the response to insulin with that in GL cells from ovulatory women with (ovPCO) and without polycystic ovaries (controls).Methods: ...
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ea0025p277 | Reproduction | SFEBES2011

Increased intensity of P450c17 protein expression in theca cells from polycystic ovaries

Comim Fabio , Hardy Kate , Franks Stephen

Background/aims: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women, being characterized by ovarian hyperandrogenism attributed to intrinsic overproduction by theca cells (TCs). Although previous reports pointed a higher stability and transcription of CYP17mRNA (Wickenheisser JK et al. 2006)1, few have examined (and none has quantified) P450c17 protein expression in TCs from PCOS ovaries. Therefore, the aim of this study is to qua...
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ea0025p278 | Reproduction | SFEBES2011

Neuromedin B stimulates the hypothalamo-pituitary–gonadal axis in male rats

Boughton Charlotte , Patel Sejal , Amin Anjali , Thompson Emily , Ghatei Mohammad , Bloom Stephen , Murphy Kevin

Neuromedin B (NMB) is a highly conserved bombesin-related peptide found in mammals. The mammalian bombesin family of receptors consists of three closely related G protein coupled receptors, BB1, BB2 and BB3. The BB1 receptor subtype has the highest affinity for NMB. NMB mRNA is detected in the CNS and is expressed at relatively high levels in the rat hypothalamus, in particular the medial preoptic area and the arcuate nucleus.<p ...
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ea0025p279 | Reproduction | SFEBES2011

Nesfatin stimulates the hypothalamic–pituitary–gonadal axis in male rats

Patterson Michael , Wynne Katie , Patel Sejal , Suzuki Keisuke , Tadross John , Ghatei Mohammad , Bloom Stephen

Nesfatin is an 82 amino acid peptide identified as a novel hypothalamic regulator of feeding. In rodents, central administration of nesfatin acutely inhibits feeding and chronic administration reduces weight gain. Subsequent research has demonstrated nesfatin is involved in the control of puberty in female rats. During puberty i.c.v. administration of nesfatin stimulates release of LH and FSH but has no effect in adult female rats.We investigated the eff...
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ea0025p280 | Reproduction | SFEBES2011

Study of role of retinol-binding protein 4 in women with polycystic ovary syndrome

Eldieb Abdel-Sattar , Makboul Khalid , Abd El Baki Rania

Background: Obesity is a common feature of women with PCOS in different ethnic cohorts. It is suggested that retinol binding protein 4 (RBP4) is a central mediator of obesity-induced insulin resistance in mice and humans. In addition, elevated serum RBP4 levels have been associated with cardiovascular risk factors and metabolic syndromes.Aim: To investigate whether serum RBP4 level is correlated with metabolic parameters, indices of insulin resistance, a...
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ea0025p281 | Reproduction | SFEBES2011

HRPE773 (ZG16B) expression is elevated in human endometrium during the early secretory phase of the menstrual cycle and in uterine decidua following miscarriage

Ng Bonnie , McDonald Sarah , Ren Xia , Mullins John , Rae Michael , Critchley Hillary , Horne Andrew , Morley Steven

Introduction: Expression of murine CSP-1/Dcpp secretory proteins was first identified in sublingual salivary glands and subsequently in secretory epithelia of several other tissues, including the mouse female reproductive tract where expression is regulated by oestrogen. Preliminary studies indicated that HRPE773, the human CSP-1/Dcpp orthologue, displayed a similar pattern of expression to its murine counterparts. We therefore hypothesized that HRPE773 might be expressed in h...
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ea0025p282 | Reproduction | SFEBES2011

Case–control study of 5-alpha reductase type 2 Val89Leu polymorphism in Romanian PCOS patients

Radian Serban , Radulescu Cristina Ramona , Aflorei Daniela , Gheorghiu Monica , Baculescu Nicoleta , Albu Alice , Fica Simona , Grigorescu Florin , Coculescu Mihail

Background: Hyperandrogenism (HA) is required for diagnosis of polycystic ovary syndrome (PCOS) and it is central to PCOS pathogenesis. Putative mechanisms of hyperandrogenism include disregulation of steroidogenesis, plasma transport and peripheral tissue regulation. Therefore, 5-alpha reductase genes (SRD5A1, SRD5A2), that regulate peripheral tissue conversion of testosterone to more potent dihydrotestosterone are good PCOS candidate genes. The single published study of SRD5...
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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