Inflammation is a component of obesity-associated insulin resistance. Significant increases in inflammatory mediators such as IL6, TNFa and IL1b have been detected in the serum of diabetic patients. Herein, we investigated whether pre-diabetic patients also exhibited perturbations in circulating pro-inflammatory cytokines. To this end, we recruited 42 healthy non diabetics and subjected them to 75 g OGTT after an overnight fast. We profiled the cytokines that are present in the serum of non diabetics (n=33, BMI 27.3±3.6, HbA1c 5.5±0.2%) and compare to pre-diabetic patients (n=9, BMI 33.2±8.5, HbA1c 6.0±0.3%) by multiplex cytokine profiling.
Our data clearly show for the first time that levels of serum interferon-β (IFN-b) are significantly increased in pre-diabetic patients. In contrast, comparable levels of IL6, TNFa and IL1b are evident in the serum of pre-diabetic patients. Together, these data demonstrate that pre-diabetic patients exhibit perturbations in cytokine levels compared to normal individuals and support a role for these molecules in the disease progression to a diabetic pathology. Supported by HRB and SFI Ireland.
|Cytokine (pg/ml)||Normal (mean±S.D.)||Pre-diabetic (mean±S.D.)|