SFEBES2011 Poster Presentations Cytokines, growth factors, neuroendocrinology and behaviour (11 abstracts)
Cytokine profiling of pre-diabetic patients
Saket Gupta 1, , Ashwini Maratha 1, , Thusitha Gajanayake 1, , Jakub Siednienko 1, , Anandan Natarajan 1, , Shu Hoashi 1, & Sinead Miggin 1,
1Immune Signalling Laboratory, Institute of Immunology, National University of Ireland, Maynooth, Co. Kildare, Ireland; 2Midlands Regional Hospital, Mullingar and Royal College of Surgeons in Ireland, Mullingar, Co. Westmeath, Ireland.
Inflammation is a component of obesity-associated insulin resistance. Significant increases in inflammatory mediators such as IL6, TNFa and IL1b have been detected in the serum of diabetic patients. Herein, we investigated whether pre-diabetic patients also exhibited perturbations in circulating pro-inflammatory cytokines. To this end, we recruited 42 healthy non diabetics and subjected them to 75 g OGTT after an overnight fast. We profiled the cytokines that are present in the serum of non diabetics (n=33, BMI 27.3±3.6, HbA1c 5.5±0.2%) and compare to pre-diabetic patients (n=9, BMI 33.2±8.5, HbA1c 6.0±0.3%) by multiplex cytokine profiling.
Our data clearly show for the first time that levels of serum interferon-β (IFN-b) are significantly increased in pre-diabetic patients. In contrast, comparable levels of IL6, TNFa and IL1b are evident in the serum of pre-diabetic patients. Together, these data demonstrate that pre-diabetic patients exhibit perturbations in cytokine levels compared to normal individuals and support a role for these molecules in the disease progression to a diabetic pathology. Supported by HRB and SFI Ireland.
| Cytokine (pg/ml) | Normal (mean±S.D.) | Pre-diabetic (mean±S.D.) |
| IL6 | 1.14±0.59 | 1.59±0.59 |
| TNFa | 14.28±1.24 | 16.80±1.86 |
| IL1b | 0.84±0.41 | 0.91±0.69 |
| IFNb | 1.29±1.2 | 5.54±2.09 |
Volume 25
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more