Endocrine Abstracts

Early events in activated by adrenaline (10−6 M) and phenylephrine (10−5M) single hepatocytes of rat have been investigated by quantitative image microfluorometry and microspectrofluorometry. Membrane potential-sensitive cationic DiOC2(3) and anionic SqSC4 probes have been used for image analysis and transmenbrane potential (Δψp) estimation in real-time studies. Fluorescence spectra resulting from the accumulati...

The sequence–structure–function-analysis of glycoprotein hormone receptors (SSFA-GPHR) database provides a comprehensive set of mutation data for the glycoprotein hormone receptors (covering the lutropin – (LHCGR), the follitropin (FSHR) and the thyrotropin receptor (TSHR)). Numerous databases present functional information along with pooled data from various public resources, but tools to analyse the molecular effects of genetic variations are as yet poorly pro...

The aim of our study is to identify signalling sensitive residue positions of the glycoprotein hormone receptors (GPHR), especially the thyrotropin receptor (TSHR) on the transmembrane helices (TMH) 5 and 6, knowing to be involved in GPCR activation. We performed modelling driven site-directed mutagenesis to pinpoint residues that are responsible for stabilization of active and inactive conformations of the TSHR.We highlight two amino acids that are high...

Introduction: 3-Iodothyronamine (T1AM) is a novel endogenous relative of thyroid hormone able to interact with specific G protein-coupled receptors, known as trace amine-associated receptors (TAAR). Metabolic, endocrine and cardiac effects of exogenous T1AM have been reported.Methods: We synthesized radiolabelled T1AM, [125I]-T1AM, and explored its distribution in different mouse organs after injection in the tail vein. The specificity of [<su...