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15th International & 14th European Congress of Endocrinology

Oral Communications

Diabetes Basic

ea0029oc17.1 | Diabetes Basic | ICEECE2012

Pdk1/Foxo1 pathway regulates macrophage migration, polarization, and insulin sensitivity

Kawano Y. , Nakae J. , Itoh H.

Chronic inflammation in adipose tissue contributes to obesity-related insulin resistance. The 3-Phosphoinositide-dependent Protein Kinase 1 (Pdk1)/forkhead transcription factor (Foxo1) pathway is important in regulating glucose and energy homeostasis, but little is known about this pathway in adipose tissue macrophages (ATMs). To investigate this, we generated transgenic mice that carried macrophage/granulocyte-specific mutations, including a Pdk1 knockout (LysMPdk1-/-), a Pdk...

ea0029oc17.2 | Diabetes Basic | ICEECE2012

Placental restriction co-ordinately alters hepatic expression of microRNAs and targets related to non alcoholic fatty liver disease, in adult male sheep

Harryanto H. , De Blasio M. , Grant P. , Gatford K. , Owens J.

Placental restriction (PR) of fetal growth impairs insulin action and metabolic control in postnatal life. MicroRNAs (miRNAs) are important post-transcriptional regulators, down regulating mRNA abundance or translation. We therefore determined if PR in the sheep alters miR expression in liver, skeletal muscle and adipose tissue of adult offspring and if this associates with altered abundance of their predicted molecular targets.PR was induced by pre-preg...

ea0029oc17.3 | Diabetes Basic | ICEECE2012

Caerulin precursor fragment (CPF-AM1): a novel insulinotropic peptide from the skin secretion of the clawed frog, Xenopus amieti

Ojo O. , Conlon J. , Flatt P. , Abdel-Wahab Y.

We reported the isolation and structural characterization of CPF-AM1 and other peptides from the skin secretion of Xenopus amieti. This study investigated the insulin-releasing activities of synthetic CPF-AM1 using clonal pancreatic cell line, BRIN-BD11 and Swiss TO mice with diet-induced insulin resistance.Acute insulin-release studies were performed in Krebs Ringer bicarbonate buffer supplemented with 5.6 mM or 16.7 mM glucose, purified synthetic pepti...

ea0029oc17.4 | Diabetes Basic | ICEECE2012

Syntaxin-3 regulates recruitment and exocytosis of newcomer insulin granules and granule-granule fusion during biphasic glucose-stimulated insulin secretion in pancreatic β-cells

Zhu D. , Koo E. , Kwan E. , Kang Y. , Park S. , Xie H. , Sugita S. , Gaisano H.

Introduction: The molecular basis of exocytosis of secretory insulin-containing granules (SGs) during biphasic glucose-stimulated insulin secretion (GSIS) of pancreatic β-cells remains unclear. Syntaxins (Syns) Syn-1A and Syn-4 are t-SNARE proteins situated on the plasma membrane that have been well studied to mediate insulin exocytosis. However, Syn-3, peculiarly more abundant in insulin SGs, has unclear function in GSIS.Methods/Designs: Confocal m...

ea0029oc17.5 | Diabetes Basic | ICEECE2012

Glucocorticoid receptor α expression is downregulated in gluteal subcutaneous adipose tissue of black South African women and associates with increased metabolic risk

Goedecke J. , Stimson R. , Livingstone D. , Hayes P. , Adams K. , Dave J. , Keswell D. , Evans J. , Victor H. , Lambert E. , Levitt N. , Kahn S. , Olsson T.

Introduction: Increased capacity for glucocorticoid regeneration in subcutaneous adipose tissue (SAT) by 11β-hydroxysteroid dehydrogenase-1 (11HSD1) is associated with obesity and associated risk factors. We hypothesised that down-regulation of SAT 11HSD1 and/or glucocorticoid receptor-α (GRα) may explain differences in body fat distribution and metabolic risk between black and white women. The study aimed to compare the expression of 11HSD1 and glucocorticoid r...

ea0029oc17.6 | Diabetes Basic | ICEECE2012

Meta-analysis of differentially expressed microRNAs in type 1, type 2 and gestational diabetes mellitus

Collares C. , Evangelista A. , Xavier D. , Macedo C. , Rassi D. , Foss-Freitas M. , Foss M. , Sakamoto-Hojo E. , Passos G. , Donadi E.

Introduction: MicroRNAs (miRNA) are noncoding RNAs that play a central role in governing a variety of physiological and pathological processes, and there are few studies on miR involvement in diabetes mellitus. There are three principal types of diabetes, i.e., type 1 (T1DM), type 2 (T2DM) and gestational diabetes mellitus (GDM). In this study we performed a meta-analysis of the miRNA expression profiling of peripheral blood mononuclear cells (PBMC) from T1DM, T2DM and GDM pat...