Searchable abstracts of presentations at key conferences in endocrinology
Endocrine Abstracts (2012) 29 P48

ICEECE2012 Poster Presentations Adrenal cortex (113 abstracts)

MicroRNA profiling of benign and malignant adrenocortical tumors reveals potential biomarkers of recurrence

O. Chabre 1 , G. Assié 2 , R. Libé 2 , J. Bertherat 2 , J. Feige 3 & N. Cherradi 3


1Service d’Endocrinologie, Grenoble, France; 2INSERM U567, CNRS UMR8104, Paris, France; 3INSERM U1036, Grenoble, France.

Objective: To identify miRNAs predictors of poor prognosis in adrenocortical cancer.

Methods: Using microarrays, we evaluated the expression of 728 human miRNAs in six adenomas (ACAs) and twelve carcinomas (ACCs). The ACC group was composed of two subgroups A and B consisting of six recurrent (subgroup A) and six non-recurrent tumors (subgroup B). These two distinct subgroups have been characterized recently (de Reynies et al, 2009) on the basis of distinct gene expression profiles: comparison of global survival revealed a major difference in outcome in these two subtypes of ACC, with a very high rate of relapse and death within two years following surgery in the A subgroup.

Results: Twelve miRNAs were differentially expressed between ACCs and ACAs, 5 of which were down-regulated and 7 up-regulated in ACCs. The best discriminatory miRNAs between ACCs and ACAs were miR-195 and miR-335 which were down-regulated in ACCs. 29 miRNAs were differentially expressed between the two ACC subgroups A and B, with all discriminatory miRNAs more strongly expressed in A than in B carcinoma samples. Among them, miR-139-5p was the most powerful discriminatory miRNA between A and B subtypes with consistent up-regulation in recurrent carcinoma (A). Quantitative RT-PCR revealed that the levels of expression of miR-195 and miR-335 were similar in ACAs and normal adrenal cortex while strongly repressed in ACCs. Overexpression of miR-139-5p was confirmed in ACCs type A. These results were validated in a separate cohort of ten benign and twenty malignant samples (10 ACCs type A and 10 ACCs type B) using quantitative RT-PCR. Target prediction analysis revealed that predicted targets of these miRNAs are involved in biological processes which enhance tumor progression.

Conclusions: Our data suggest that adrenocortical cancer cells progressively switch from a high miR-195 and miR-335 status to a low miR-195 and miR-335 phenotype. miR-139-5P is a potential prognostic biomarker of recurrent ACCs.

Declaration of interest: The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project.

Funding: This work was supported, however funding details unavailable.

Volume 29

15th International & 14th European Congress of Endocrinology

European Society of Endocrinology 

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