Searchable abstracts of presentations at key conferences in endocrinology
Endocrine Abstracts (2013) 31 P302 | DOI: 10.1530/endoabs.31.P302

SFEBES2013 Poster Presentations Pituitary (71 abstracts)

Effect of t-2 toxin and combination of t-2 toxin with resveratrol and mc2183 on ovarian cells of japanese quails

Nora Maruniakova 1 , Attila Kadasi 1 , Marina Medvedova 1 , Marek Halenar 1 , Jozef Bulla 1 , Alexander V Sirotkin 2 , Roland Grossmann 3 & Adriana Kolesarova 1

1Slovak University of Agriculture, Nitra, Slovakia; 2Animal Production Research Centre Nitra, Luzianky-Nitra, Slovakia; 3Institute of Animal Science, Mariensse, Germany.

Occurence of mycotoxins in various food and feed commodities is a worldwide problem. T-2 toxin is one of the most toxic trichothecene mycotoxin, produced mainly by Fusarium species. Poultry belongs to very sensitive species which are very often explored to toxic effects of mycotoxins. The aim of our in vitro studies was to examine secretion activity of ovarian granulossa cells to produce progesterone P4 after T-2 toxin and its combinations with resveratrol (RSV) and synthetic mTOR blocker MC2183 addition. Ovarian granulosa cells were incubated without (control group)/with treatments of natural substances at various doses for 24 h: T-2 toxin (10 100 1000 ng/ml), T-2 toxin (10 100 1000 ng/ml) plus resveratrol (60 μg/ml) and T-2 toxin (10 100 1000 ng/ml) plus MC2183 (60 μg/ml). Secretion of progesterone after addition of T-2 toxin and combinations of T-2 toxin with resveratrol or MC2183 was determined by RIA. Progesterone release by GC was stimulated by T-2 toxin at the doses 1000 ng/ml but P4 was inhibited by addition at 100, 10 ng/ml. Stimulation of P4 output at all used doses was after addition of combination of T-2 toxin (10 100 1000 ng/ml) plus RSV (60 μg/ml). On the other hand, inhibitory effect was after addition of T-2 toxin (10 100 ng/ml) in combination with MC2183 (60 μg/ml). However at highest used doses T-2 toxin (1000 ng/ml) plus MC2183 (60 μg/ml) we observed stimulation in P4 release by ovarian granulosa cells. All our observed results were not significant (P≧0.05). In conclusion, our results suggest possible effect of T-2 toxin and its combination with RSV or MC2183 on ovarian function.

Declaration of funding: This work was financially supported by the Ministry of Education of the Slovak Republic project no. 1/0790/11 and 1/0084/12.

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